LEADER 05697nam 2200721 450 001 9910811080903321 005 20230125234159.0 010 $a1-5231-4635-4 010 $a1-59693-407-7 035 $a(CKB)2550000000000251 035 $a(EBL)946541 035 $a(OCoLC)796382984 035 $a(SSID)ssj0000340358 035 $a(PQKBManifestationID)12084458 035 $a(PQKBTitleCode)TC0000340358 035 $a(PQKBWorkID)10387009 035 $a(PQKB)10836305 035 $a(Au-PeEL)EBL946541 035 $a(CaPaEBR)ebr10358996 035 $a(CaBNVSL)mat09100472 035 $a(IEEE)9100472 035 $a(MiAaPQ)EBC946541 035 $a(EXLCZ)992550000000000251 100 $a20200729d2009 uy 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMethods in bioengineering $esystems analysis of biological networks /$fArul Jayaraman, Juergen Hahn, editors 210 1$aBoston [Mass.] :$cArtech House,$dİ2009. 210 2$a[Piscataqay, New Jersey] :$cIEEE Xplore,$d[2009] 215 $a1 online resource (328 p.) 225 1 $aArtech House methods in bioengineering series 300 $aDescription based upon print version of record. 311 $a1-59693-406-9 320 $aIncludes bibliographical references and index. 327 $aMethods in Bioengineering: Systems Analysis of Biological Networks; Contents; Chapter 1 Quantitative Immunofluorescence for Measuring Spatial Compartmentation of Covalently Modified Signaling Proteins; 1.1 Introduction; 1.2 Experimental Design; 1.3 Materials; 1.3.1 Cell culture; 1.3.2 Buffers/reagents; 1.3.3 Immunofluorescence reagents; 1.4 Methods; 1.4.1 Cell culture and stimulation for phospho-ERK measurements; 1.4.2 Antibody labeling of phosphorylated ERK (ppERK); 1.4.3 Fluorescence microscopy imaging of ppERK and automated imageanalysis 327 $a1.5 Data Acquisition, Anticipated Results, and Interpretation1.6 Statistical Guidelines; 1.7 Discussion and Commentary; 1.8 Application Notes; 1.9 Summary Points; Acknowledgments; References; Chapter 2 Development of Green Fluorescent Protein-Based Reporter Cell Lines for Dynamic Profiling of Transcription Factor and Kinase Activation; 2.1 Introduction; 2.2 Materials; 2.2.1 Cell and bacterial culture; 2.2.2 Buffers and reagents; 2.2.3 Cloning; 2.2.4 Microscopy; 2.3 Methods; 2.3.1 3T3-L1 cell culture; 2.3.2 Transcription factor reporter development; 2.3.3 Kinase reporter development 327 $a2.4 Application Notes2.4.1 Electroporation of TF reporter plasmids into 3T3-L1 preadipocytes; 2.4.2 Monitoring activation of ERK in HepG2 cells; 2.5 Data Acquisition, Anticipated Results, and Interpretation; 2.6 Discussion and Commentary; 2.7 Summary Points; Acknowledgments; References; Chapter 3 Comparison of Algorithms for Analyzing Fluorescent Microscopy Images and Computation of Transcription Factor Profiles; 3.1 Introduction; 3.2 Preliminaries; 3.2.1 Principles of GFP reporter systems; 3.2.2 Wavelets; 3.2.3 K-means clustering; 3.2.4 Principal component analysis 327 $a3.2.5 Mathematical description of digital images and image analysis3.3 Methods; 3.3.1 Image analysis based on wavelets and a bidirectional search; 3.3.2 Image analysis based on K-means clustering and PCA; 3.3.3 Determining fluorescence intensity of an image; 3.3.4 Comparison of the two image analysis procedures; 3.4 Data Acquisition, Anticipated Results, and Interpretation; 3.4.1 Developing a model describing the relationship between the transcription factor concentration and the observed fluorescence intensity 327 $a3.4.2 Solution of an inverse problem for determining transcription factor concentrations3.5 Application Notes; 3.6 Summary and Conclusions; Acknowledgments; References; Chapter 4 Data-Driven, Mechanistic Modeling of Biochemical Reaction Networks; 4.1 Introduction; 4.2 Principles of Data-Driven Modeling; 4.2.1 Types of experimental data; 4.2.2 Data processing and normalization; 4.2.3 Suitability of models used in conjunction with quantitative data; 4.2.4 Issues related to parameter specification and estimation; 4.3 Examples of Data-Driven Modeling 327 $a4.3.1 Example 1: Systematic analysis of crosstalk in the PDGF receptor signaling network 330 3 $aThis practical book is part of the new Artech House Methods in Bioengineering series - volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Written and edited by recognized experts in the field, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This volume focuses on the design of state-of-the art methods for investigating complex biological systems and the development of complex models to analyze the data. Professionals find how-to guidance on experimental approaches for investigating cellular behavior in health and disease.$cPublisher abstract. 410 0$aArtech House methods in bioengineering series. 517 3 $aSystems analysis of biological networks 606 $aBacterial genetics 606 $aCytology$xTechnique 606 $aViral genetics 615 0$aBacterial genetics. 615 0$aCytology$xTechnique. 615 0$aViral genetics. 676 $a610.28 676 $a660.6 701 $aJayaraman$b Arul$01670902 701 $aHahn$b Juergen$0223330 801 0$bCaBNVSL 801 1$bCaBNVSL 801 2$bCaBNVSL 906 $aBOOK 912 $a9910811080903321 996 $aMethods in bioengineering$94033076 997 $aUNINA