LEADER 03071nam 2200601 450 001 9910810329203321 005 20230801233223.0 010 $a1-4738-1946-6 010 $a1-4738-0014-5 035 $a(CKB)3710000000125571 035 $a(EBL)1718493 035 $a(OCoLC)881887679 035 $a(SSID)ssj0001294154 035 $a(PQKBManifestationID)11841624 035 $a(PQKBTitleCode)TC0001294154 035 $a(PQKBWorkID)11317216 035 $a(PQKB)10422613 035 $a(MiAaPQ)EBC1718493 035 $a(Au-PeEL)EBL1718493 035 $a(CaPaEBR)ebr10884673 035 $a(CaONFJC)MIL623687 035 $a(EXLCZ)993710000000125571 100 $a20140704e20122011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 14$aThe last Prussian $ea biography of field marshal Gerd Von Rundstedt, 1875-1953 /$fCharles Messenger 210 1$aSouth Yorkshire, England :$cPen & Sword Military,$d2012. 210 4$dİ2011 215 $a1 online resource (414 p.) 300 $aDescription based upon print version of record. 311 $a1-84884-662-2 320 $aIncludes bibliographical references and index. 327 $a""Cover""; ""Half Title""; ""Title""; ""Copyright""; ""Contents""; ""LIST OF MAPS""; ""CHRONOLOGY""; ""Introduction""; ""Introduction to the New Edition""; ""1. Early Life""; ""2. The First World War""; ""3. The Weimar Years""; ""4. Enter Hitler""; ""5. Poland""; ""6. Assault in the West""; ""7. Invasion Talk""; ""8. Russia 1941""; ""9. Return to France""; ""10. Normandy and the Bomb Plot""; ""11. Recalled Once More""; ""12. The Last Battles""; ""13. Prisoner of War""; ""14. War Criminal""; ""15. The Twilight Years""; ""16. The Reckoning""; ""APPENDIX ONE THE VON RUNDSTEDT FAMILY TREE"" 327 $a""APPENDIX TWO DECORATIONS AWARDED TO GERD VON RUNDSTEDT""""SOURCE NOTES""; ""SELECT BIBLIOGRAPHY""; ""INDEX"" 330 $aField Marshal Gerd von Rundstedt (1875-1953) was one of the foremost German commanders of the Second World War. After service on both the Western and Eastern Fronts during 1914-1918 he rose steadily through the ranks before retiring in 1938. Recalled to plan the attack on Poland, he played a leading part in this and the invasion of France in 1940. Thereafter he commanded Army Group South in the assault on Russia before being sacked at the end of 1941. Recalled again, he was made Commander-in-Chief West and as such faced the 1944 Allied invasion of France, but was removed that July. He resumed 606 $aWorld War, 1939-1945$zGermany 606 $aMarshals$zGermany$vBiography 607 $aGermany$xArmed Forces$xHistory$y20th century 607 $aGermany$xArmed forces$vBiography 615 0$aWorld War, 1939-1945 615 0$aMarshals 676 $a355.331092 700 $aMessenger$b Charles$0591848 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910810329203321 996 $aThe last Prussian$94084380 997 $aUNINA LEADER 05178nam 2200661Ia 450 001 9911019839903321 005 20200520144314.0 010 $a9786610519712 010 $a9781280519710 010 $a1280519711 010 $a9783527604395 010 $a3527604391 010 $a9783527604371 010 $a3527604375 035 $a(CKB)1000000000377164 035 $a(EBL)482269 035 $a(OCoLC)68617526 035 $a(SSID)ssj0000238803 035 $a(PQKBManifestationID)11228463 035 $a(PQKBTitleCode)TC0000238803 035 $a(PQKBWorkID)10239246 035 $a(PQKB)10399275 035 $a(MiAaPQ)EBC482269 035 $a(Perlego)2774506 035 $a(EXLCZ)991000000000377164 100 $a20040722d2005 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aRNA interference in practice /$fUte Schepers 210 $aWeinheim $cWiley-VCH$d2005 215 $a1 online resource (338 p.) 300 $aDescription based upon print version of record. 311 08$a9783527310203 311 08$a3527310207 320 $aIncludes bibliographical references and index. 327 $aRNA Interference in Practice; Preface; Contents; 1 Introduction: RNA interference, the ""Breakthrough of the Year 2002""; 1.1 RNAi as a Tool for Functional Genomics; 1.2 Mechanism of RNAi; 1.3 Dicer - the Initiator to "Dice" the dsRNA?; 1.4 miRNAs versus siRNAs: Two Classes of Small RNAs Using the RNAi Pathway?; 1.5 RISC - the Effector to "Slice" the mRNA?; 1.6 Are RNA-Dependent RNA Polymerases (RdRps) Responsible for the Catalytic Nature of RNAi?; 1.7 Is RNAi Involved in the Regulation of Gene Expression?; 1.8 RNAi in Mammals; 1.9 Practical Approaches; 1.10 References 327 $a2 RNAi in Caenorhabditis elegans2.1 Introduction; 2.2 Application of RNAi in C. elegans; 2.3 Target Sequence Evaluation; 2.4 dsRNA Synthesis; 2.4.2 Generation of the DNA Template; 2.4.2.1 Plasmid Templates; 2.4.2.2 DNA Templates Derived by PCR/RT-PCR; 2.4.2 In-vitro dsRNA Transcription; 2.5 Delivery of dsRNA; 2.5.1 General Information on the C. elegans Anatomy; 2.5.2 C. elegans Strains for Silencing; 2.5.3 Culturing the Worms; 2.5.4 Microinjection Protocol; 2.5.5 Soaking Protocol; 2.5.6 RNAi Feeding Protocol; 2.5.7 DNA Templates for dsRNA Expression in Feeding E. coli 327 $a2.5.8 DNA Templates for Hairpin RNA Expression2.5.8.1 C. elegans Promoters; 2.5.8.2 Inverted Repeat Constructs; 2.6 Mounting Animals for Microscopy; 2.7 Genome Wide Screens; 2.7.1 C. elegans RNAi Library; 2.8 Selected Literature on C. elegans Research; 2.9 Useful C. elegans webpages; 2.10 References; 3 RNAi in Drosophila; 3.1 Introduction; 3.2 Application of RNAi in Drosophila; 3.2.1 dsRNA from Linear DNA Templates; 3.2.2 dsRNA from Inverted Repeat DNA; 3.2.3 Inducible Expression in Drosophila Cell Lines; 3.2.4 Limitations; 3.3 dsRNA Synthesis; 3.3.1 In-vitro dsRNA Transcription 327 $a3.3.2 Inverted Repeat DNA3.4 Injections; 3.4.1 Injection Services; 3.4.2 Injection Method; 3.4.3 DsRNA or Inverted Repeat DNA Preparation; 3.4.4 Embryo Collection; 3.5 Cell Lines; 3.6 Protocols; 3.6.1 Thawing and Maintenance of S2 Cells; 3.6.2 Freezing Protocol; 3.7 RNAi in S2 Cells; 3.7.1 dsRNA Transfection Using the Calcium Phosphate Method; 3.7.2 dsRNA Soaking of S2 Cells; 3.8 High-Throughput Screens; 3.8.1 Drosophila RNAi Library; 3.9 Useful Webpages for Drosophila Research; 3.10 Books and Literature on Drosophila; 3.11 References; 4 RNAi in Mammals; 4.1 Introduction 327 $a4.2 Transient RNAi in Cell Culture4.2.1 Chemical Synthesis and Modifications of siRNAs; 4.2.1.1 Advantages; 4.2.1.2 Limitations; 4.2.2 Custom Synthesis of siRNA Oligos; 4.2.3 siRNA Design Rules; 4.2.3.1 siRNA Strand Bias and Off-Gene Targeting; 4.2.3.2 Improvements in siRNA Stability; 4.2.3.3 siRNA Design: Novel Modifications of the "Tuschl Rules"; 4.2.3.4 Homology Search by BLAST, FASTA, or Smith-Waterman Algorithm; 4.2.3.5 Troubleshooting; 4.2.3.6 siRNA Design Programs and Algorithms; 4.2.3.7 Preparation of siRNA Duplexes; 4.2.4 Enzymatic Synthesis of siRNAs 327 $a4.2.4.1 Designing DNA Oligonucleotides 330 $aThis hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids.In easy-to-follow, step-by-step protocols you will learn* how RNAi works in worms, flies and mammals,* how to design the most efficient RNAi constructs,* how to achieve transient, stable and conditional RNAi in cell cultures,* how to determine the efficiency of an RNAi experiment,* and how to use RNAi for gene therapy.All the protocols have been thoroughly tested in the author's own laboratory, and she provides exa 606 $aRNA editing 606 $aGenetic transcription 615 0$aRNA editing. 615 0$aGenetic transcription. 676 $a572.8845 700 $aSchepers$b Ute$01842247 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9911019839903321 996 $aRNA interference in practice$94422266 997 $aUNINA