LEADER 04895nam 2200625 450 001 9910808380103321 005 20230911182125.0 010 $a1-119-48401-4 010 $a1-119-48397-2 010 $a1-119-48398-0 035 $a(CKB)4100000007805059 035 $a(MiAaPQ)EBC5726274 035 $a(JP-MeL)3000132245 035 $a(Au-PeEL)EBL5726274 035 $a(PPN)242675549 035 $a(OCoLC)1082322039 035 $a(CaSebORM)9781119483960 035 $a(EXLCZ)994100000007805059 100 $a20190328d2019 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aBiomolecular and bioanalytical techniques $etheory, methodology and applications /$fedited by Vasudevan Ramesh, School of Chemistry, University of Manchester, Manchester,U.K 205 $a1st edition 210 1$aHoboken, NJ :$cWiley,$d2019. 215 $a1 online resource (579 pages) 300 $aIncludes bibliographical references and index 311 $a1-119-48396-4 320 $aIncludes bibliographical references and index. 327 $aPrinciples of health and safety and good laboratory practice -- Applications of chemoinformatics in drug discovery -- Bioinformatics and its applications in genomics -- Gene cloning for the analysis of gene expression -- Proteomic techniques and their applications -- Overproduction,separation and purification of affinity-tagged proteins from escherichia coli -- Chromatography: separation techniques in biology -- Synthetic methodology in chemical biology -- Reaction chemical kinetics in biology -- Mass spectrometry and its applications -- Applications and complementarity of analytical ultracentrifugation and light-scattering techniques -- Application of isothermal titration calorimetry (ITC) to biomolecular interactions -- An introduction to infrared and raman spectroscopies for pharmaceutical and biomedical studies -- Fluorescence spectroscopy and its applications in analyzing biomolecular processes -- Circular dichroism and related spectroscopic techniques -- Principles and practice in macromolecular X-ray crystallography -- Biomolecular NMR spectroscopy and structure determination of DNA -- Cryo-TEM and biological structure determination -- Computer modelling and molecular dynamics simulation of biomolecules. 330 $aAn essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique?s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field. 606 $aMolecular biology$xTechnique 606 $aBiophysics 606 $aMedical Informatics 606 $aBiomedical Technology 606 $aCheminformatics 606 $aDrug Discovery 615 0$aMolecular biology$xTechnique. 615 2$aBiophysics. 615 2$aMedical Informatics. 615 2$aBiomedical Technology 615 2$aCheminformatics. 615 2$aDrug Discovery. 676 $a572.8/38 686 $a464.1$2njb/09 702 $aRamesh$b Vasudevan 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910808380103321 996 $aBiomolecular and bioanalytical techniques$94034936 997 $aUNINA