LEADER 02605oam 2200601I 450 001 9910800200003321 005 20230803030144.0 010 $a0-429-11045-6 010 $a1-4398-4813-0 024 7 $a10.1201/b14930 035 $a(CKB)2670000000359900 035 $a(EBL)1190579 035 $a(SSID)ssj0000876789 035 $a(PQKBManifestationID)11467572 035 $a(PQKBTitleCode)TC0000876789 035 $a(PQKBWorkID)10904439 035 $a(PQKB)10259615 035 $a(MiAaPQ)EBC1190579 035 $a(Au-PeEL)EBL1190579 035 $a(CaPaEBR)ebr10706067 035 $a(CaONFJC)MIL502331 035 $a(OCoLC)845257664 035 $a(OCoLC)855711925 035 $a(EXLCZ)992670000000359900 100 $a20180331d2013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aPCR technology $ecurrent innovations /$fedited by Tania Nolan, Stephen A. Bustin 205 $a3rd ed. 210 1$aBoca Raton :$cTaylor & Francis,$d2013. 215 $a1 online resource (457 p.) 300 $aDescription based upon print version of record. 311 $a1-4398-4805-X 320 $aIncludes bibliographical references. 327 $apt. 1. Template preparation -- pt. 2. Reaction components -- pt. 3. Instruments -- pt. 4. Design, optimization, QC, and standardization -- pt. 5. Data analysis -- pt. 6. Applications. 330 $aA technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution--Provided by publisher. 606 $aDNA polymerases 606 $aTransferases 615 0$aDNA polymerases. 615 0$aTransferases. 676 $a572.8/636 701 $aNolan$b Tania$01588503 701 $aBustin$b Stephen A.$f1954-$01588504 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910800200003321 996 $aPCR technology$93878376 997 $aUNINA