LEADER 04133oam 2200601I 450 001 9910800194403321 005 20230807204134.0 010 $a0-429-19375-0 010 $a1-4398-6167-6 024 7 $a10.1201/b17018 035 $a(CKB)2670000000557116 035 $a(EBL)1598078 035 $a(SSID)ssj0001193998 035 $a(PQKBManifestationID)11670925 035 $a(PQKBTitleCode)TC0001193998 035 $a(PQKBWorkID)11147996 035 $a(PQKB)11046689 035 $a(OCoLC)879945850 035 $a(MiAaPQ)EBC1598078 035 $a(OCoLC)883155670 035 $a(EXLCZ)992670000000557116 100 $a20180331h20152015 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aFluorescence lifetime spectroscopy and imaging $eprinciples and applications in biomedical diagnostics /$fedited by Laura Marcu, Paul M.W. French, Daniel S. Elson 210 1$aBoca Raton :$cTaylor & Francis,$d[2015] 210 4$dİ2015 215 $a1 online resource (554 p.) 300 $aDescription based upon print version of record. 311 $a1-306-86628-6 311 $a1-4398-6168-4 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aFront Cover; Contents; Preface; Editors; Contributors; Chapter 1: Overview of fluorescence lifetime imaging and metrology; Chapter 2: Photophysics of fluorescence; Chapter 3: Tissue fluorophores and their spectroscopic characteristics; Chapter 4: Pulse sampling technique; Chapter 5: Single-point probes for lifetime spectroscopy: Time-correlated single-photon counting technique; Chapter 6: Optical instrumentation design for fluorescence lifetime spectroscopy and imaging; Chapter 7: Fluorescence lifetime imaging techniques: Frequency-domain FLIM 327 $aChapter 8: Fluorescence lifetime imaging techniques: Time-gated fluorescence lifetime imagingChapter 9: Fluorescence lifetime imaging techniques: Time-correlated single-photon counting; Chapter 10: The phasor approach to fluorescence lifetime imaging: Exploiting phasor linear properties; Chapter 11: Analysis of time-domain fluorescence measurements using least-squares deconvolution; Chapter 12: Global analysis of FLIM-FRET data; Chapter 13: Fluorescence lifetime imaging in turbid media; Chapter 14: Oncology applications: Optical diagnostics of cancer; Chapter 15: Oncology applications: Brain 327 $aChapter 16: Oncology applications: Skin cancerChapter 17: Oncology applications: Gastrointestinal cancer; Chapter 18: Oncology applications: Intraoperative diagnosis of head and neck carcinoma; Chapter 19: Fluorescence lifetime techniques in cardiovascular disease diagnostics; Chapter 20: Ophthalmic applications of FLIM; Chapter 21: Fluorescence lifetime imaging applications in tissue engineering; Chapter 22: Tomographic fluorescence lifetime imaging; Chapter 23: Photosensitizers and PDT; Chapter 24: Fluorescence lifetime imaging of ions in biological tissues; Back Cover 330 $aThis book reflects the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Fluorescence lifetime approaches maximize contrast between different fluorophores for improved specificity of measurements, enabling characterization of intact tissue and disease diagnostics. The book offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, 606 $aDiagnosis, Fluoroscopic 606 $aFluorescence spectroscopy 615 0$aDiagnosis, Fluoroscopic. 615 0$aFluorescence spectroscopy. 676 $a616.07/54 702 $aMarcu$b Laura 702 $aFrench$b Paul M. W. 702 $aElson$b Daniel S. 801 0$bFlBoTFG 801 1$bFlBoTFG 906 $aBOOK 912 $a9910800194403321 996 $aFluorescence lifetime spectroscopy and imaging$93874725 997 $aUNINA