LEADER 05432nam 2200661Ia 450 001 9910139923703321 005 20170815165108.0 010 $a1-282-68979-7 010 $a9786612689796 010 $a3-527-62719-7 010 $a3-527-62720-0 035 $a(CKB)1000000000789627 035 $a(EBL)481654 035 $a(OCoLC)441887034 035 $a(SSID)ssj0000342480 035 $a(PQKBManifestationID)11243285 035 $a(PQKBTitleCode)TC0000342480 035 $a(PQKBWorkID)10285626 035 $a(PQKB)10182309 035 $a(MiAaPQ)EBC481654 035 $a(PPN)152543341 035 $a(EXLCZ)991000000000789627 100 $a20090617d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aRNA purification and analysis$b[electronic resource] $esample preparation, extraction, chromatography /$fby Douglas T. Gjerde, Lee Hoang and David Hornby 210 $aWeinheim $cWiley-VCH ;$aChichester $cJohn Wiley [distributor]$dc2009 215 $a1 online resource (209 p.) 300 $aDescription based upon print version of record. 311 $a3-527-32116-0 320 $aIncludes bibliographical references and index. 327 $aRNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography 327 $a1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 327 $a2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 327 $a3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration 327 $aReferences4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 327 $a5.3 RNA Chromatography Conditions 330 $aThis first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs.Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis.By explaining the basic and working principle 606 $aRNA$xAnalysis$vLaboratory manuals 606 $aRNA$xPurification$vLaboratory manuals 615 0$aRNA$xAnalysis 615 0$aRNA$xPurification 676 $a572.88 676 $a572.8836 676 $a574.87/3283 700 $aGjerde$b Douglas T$0440146 701 $aHornby$b David$cDr.$0993857 701 $aHoang$b Lee$0993858 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910139923703321 996 $aRNA purification and analysis$92275718 997 $aUNINA LEADER 05114oam 2200745I 450 001 9910800061903321 005 20230725023245.0 010 $a0-429-13762-1 010 $a1-282-56102-2 010 $a9786612561023 010 $a1-4200-8895-5 024 7 $a10.3109/9781420088953 035 $a(CKB)2670000000014356 035 $a(EBL)1186497 035 $a(OCoLC)843642693 035 $a(SSID)ssj0000364282 035 $a(PQKBManifestationID)11262315 035 $a(PQKBTitleCode)TC0000364282 035 $a(PQKBWorkID)10394805 035 $a(PQKB)10050079 035 $a(MiAaPQ)EBC1186497 035 $a(MiAaPQ)EBC510086 035 $a(Au-PeEL)EBL1186497 035 $a(CaPaEBR)ebr10373300 035 $a(CaONFJC)MIL256102 035 $a(OCoLC)680614244 035 $a(Au-PeEL)EBL510086 035 $a(OCoLC)609861125 035 $a(EXLCZ)992670000000014356 100 $a20180331d2010 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aPharmaceutical computer systems validation $equality assurance, risk management and regulatory compliance /$fedited by Guy Wingate 205 $a2nd ed. 210 1$aNew York :$cInforma Healthcare,$d2010. 215 $a1 online resource (773 p.) 300 $aRev. ed. of: Computer systems validation / editor, Guy Wingate. Boca Raton, Fla. : Interpharm/CRC, c2004. 311 $a1-4200-8894-7 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Foreword to the Second Edition; Foreword to the First Edition; Preface; Contributor Biographies; Abbreviations; Contents; Chapter 1. Introduction; Chapter 2. Organization and Management; Chapter 3. Supporting Processes; Chapter 4. Prospective Verification and Validation; Chapter 5. Project Initiation and Compliance Determination; Chapter 6. Requirements Capture and Supplier (Vendor)Selection; Chapter 7. Design and Development; Chapter 8. Coding, Configuration, and Build; Chapter 9. Development Testing; Chapter 10. User Qualification and Authorization to Use 327 $aChapter 11. Operation and MaintenanceChapter 12. Phaseout and Withdrawal; Chapter 13. Electronic Records and Electronic Signatures; Chapter 14. Regulatory Inspections; Chapter 15. Compliance Strategies; Chapter 16. Capabilities, Measures, and Performance; Chapter 17. Practical Troubleshooting; Chapter 18. Concluding Remarks; Chapter 19. Case Study 1: Computerized Analytical Laboratory Systems; Chapter 20. Case Study 2: Chromatography Data Systems; Chapter 21. Case Study 3: Laboratory Information Management Systems; Chapter 22. Case Study 4: Clinical Systems 327 $aChapter 23. Case Study 5: Control and Monitoring InstrumentationChapter 24. Case Study 6: Process Control Systems; Chapter 25. Case Study 7: Manufacturing Execution Systems and Electronic Batch Records; Chapter 26. Case Study 8: Building Management Systems; Chapter 27. Case Study 9: Engineering Management Systems; Chapter 28. Case Study 10: Desktop Applications Including Spreadsheets; Chapter 29. Case Study 11: Databases; Chapter 30. Case Study 12: Electronic Document Management Systems; Chapter 31. Case Study 13: Enterprise Resource Planning Systems 327 $aChapter 32. Case Study 14: Marketing and Supply ApplicationsChapter 33. Case Study 15: IT Infrastructure and Associated Services; Chapter 34. Case Study 16: Internet/Intranet Applications; Chapter 35. Case Study 17: Medical Devices and Their Automated Manufacture; Chapter 36. Case Study 18: Blood Establishment Computer Systems; Chapter 37. Case Study 19: Process Analytical Technology; Chapter 38. Case Study 20: Computer Applications Supporting the Supply of Biotechnology Products; Glossary; Back Cover 330 $aThoroughly revised to include the latest industry developments, the Second Edition presents a comprehensive overview of computer validation and verification principles and how to put them into practice. To provide the current best practice and guidance on identifying and implementing improvements for computer systems, the text extensively reviews regulations of pharmaceuticals, healthcare products, blood processing, medical devices, clinical systems, and biotechnology. Ensuring that organizations transition smoothly to the new system, this guide explains how to implement the new GMP paradigm w 606 $aPharmaceutical industry$xManagement 606 $aPharmaceutical industry$xData processing 606 $aHealth facilities$xRisk management 606 $aRisk management$xData processing 615 0$aPharmaceutical industry$xManagement. 615 0$aPharmaceutical industry$xData processing. 615 0$aHealth facilities$xRisk management. 615 0$aRisk management$xData processing. 676 $a615.1068/4 676 $a615.10684 701 $aWingate$b Guy$01588446 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910800061903321 996 $aPharmaceutical computer systems validation$93878226 997 $aUNINA