LEADER 10837nam 22005653 450 001 9910799482203321 005 20240308194645.0 010 $a3-031-40086-0 035 $a(MiAaPQ)EBC31051207 035 $a(Au-PeEL)EBL31051207 035 $a(EXLCZ)9929510380100041 100 $a20240105d2024 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aVirus Infected Cells 205 $a1st ed. 210 1$aCham :$cSpringer International Publishing AG,$d2024. 210 4$d©2023. 215 $a1 online resource (458 pages) 225 1 $aSubcellular Biochemistry Series ;$vv.106 311 08$aPrint version: Vijayakrishnan, Swetha Virus Infected Cells Cham : Springer International Publishing AG,c2024 9783031400858 327 $aIntro -- Preface -- Contents -- Part I: General/Technical Aspects -- Chapter 1: In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview -- Introduction -- Sample Preparation -- Data Collection and Image Processing -- Integrative Techniques -- Cryo-serial Block Face Scanning Electron Microscopy -- Cryo-soft X-Ray Tomography -- Cryo-correlative Light and Electron Microscopy -- Cryo-ET Studies of Enveloped Viruses and Their Interactions Within the Host Cell -- Cryo-ET of Purified Enveloped Viruses -- Human Immunodeficiency Virus (HIV-1) -- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) -- Other Enveloped Viruses -- In Situ Cellular Cryo-ET Enables Peering into the Cell -- Probing Enveloped Viral Infections and Molecular Mechanisms of Virus-Host Interactions -- Viral Disassembly and Genome Release -- Viral Factories Are Sites of Viral Replication -- Enveloped Positive-Strand RNA Viruses -- Enveloped Negative-Strand RNA Viruses -- Viral Assembly and Egress -- Concluding Remarks and Perspectives -- References -- Chapter 2: Approaches to Evaluating Necroptosis in Virus-Infected Cells -- Introduction -- Necroptosis Pathway and Players -- Necroptosis Inhibitors -- In Vitro Necroptosis in Cultured Cells -- In Vivo Models of Necroptosis -- Cellular Patterns and Methods to Evaluate Necroptosis -- Metabolic Viability Assays -- Membrane Integrity Assays -- Necroptosis Morphology -- Immuno-techniques -- The Viruses That Activate or Inhibit Necroptosis -- DNA Viruses -- Herpesviruses -- Murine Cytomegalovirus -- Human Cytomegalovirus -- Herpes Simplex Viruses -- Poxviruses -- RNA Viruses -- Influenza A -- Reovirus -- Respiratory Syncytial Virus -- SARS-CoV-2 -- Flaviviruses -- Rotavirus -- Conclusion -- References -- Chapter 3: Apoptosis and Phagocytosis as Antiviral Mechanisms -- Introduction. 327 $aVirus Life Cycle, Classification, and Infection -- Changes in Host Cells After Viral Infection -- Alteration of Gene Expression -- Changes in Cell Surface Structures -- Apoptosis and Viral Resistance to Apoptosis -- Cellular and Humoral Immune Responses Against Virus Infection and the Possible Induction of Cytokine Storm -- Roles for Phagocytosis in the Prevention and Progression of Viral Diseases -- Phagocytosis of Virus-Infected Cells as Innate Immune Response -- Consequences of Apoptosis-Dependent Phagocytosis of Virus-Infected Cells -- Perspectives -- References -- Chapter 4: The Art of Viral Membrane Fusion and Penetration -- General Terminology and Mechanisms of How Viruses Cross Host Membranes -- Viral Membrane Fusion and Membrane Fusion Proteins -- Conformational States of Viral Fusion Proteins -- Membrane Penetration by Non-enveloped Viruses -- Proteinaceous Pore-Forming Viruses -- Viruses Utilizing Lytic Peptide-Driven Membrane Penetration -- Lipid-Modifying Membrane Rupturing Viruses -- Visualization of Viral Membrane Fusion and Penetrations Using Cryo-electron Tomography -- Endosomal Lipids as Regulators of Membrane Fusion -- Effect of Lipid Curvature -- Effect of Lipid Charge -- Host Restriction Factors Controlling Viral Membrane Fusion and Penetration by Modulating the Endosomal Lipid Composition -- Role of Viral Uncoating in Membrane Fusion -- Inhibitors of Viral Membrane Fusion -- Conclusion -- Glossary -- References -- Chapter 5: Single-Particle Tracking of Virus Entry in Live Cells -- Introduction -- Dynamic Entry of Viruses into Host Cells -- Virus-Receptor Interactions -- Virus Internalization -- Virus Transport -- Virus Uncoating -- Fluorescent Labeling of Viruses and Cellular Components -- Fluorescent Proteins -- Organic Dyes -- Quantum Dots -- Microscopy Methods and Image Processing -- Microscopy Configurations. 327 $aImage Processing -- Concluding Remarks -- References -- Chapter 6: Correlative Cryo-imaging Using Soft X-Ray Tomography for the Study of Virus Biology in Cells and Tissues -- Soft X-Ray Technique -- SXT Principles and Advantages -- SXT Data Collection -- Use of SXT in Viral Studies -- Extending the Information Content of SXT Data in the Context of Correlative Microscopy Schemes -- Sample Preparation and B24 Experimental Workflow -- SXT Correlative Schemes in the Service of Virus Research -- Reovirus -- Hepatitis C Virus -- Challenges and Prospects -- References -- Chapter 7: The Virus-Induced Cytopathic Effect -- Introduction -- Cytopathic Effects across Viral Species -- Approaches to Studying the Cytopathic Effect -- Bright-Field Microscopy -- Fluorescence Microscopy -- Immunofluorescence Assay (IFA) -- Labeling with Fluorescent Dyes -- Plaque Assay -- Monitoring of the Cytopathic Effect and Its Relevance -- Cellular Tropism Evaluation -- Antiviral Drug Screening -- Neutralizing Antibodies Assessment -- Conclusions -- References -- Part II: Specific Viruses -- Chapter 8: Human Papilloma Virus-Infected Cells -- Introduction -- HPV Entry into Its Target Cells -- HPV Endocytosis as a Journey to the Nucleus -- Replication and Transcription of the HPV Genome -- Exocytosis and Exit toward a New Infection -- Conclusions -- References -- Chapter 9: Defining the Assembleome of the Respiratory Syncytial Virus -- Respiratory Syncytial Virus Infection Is a Global Health Problem -- The Genetic Structure of RSV -- RSV Particle Morphogenesis at the Assembleome -- Interactions between the Virus Membrane Structural Proteins at the Assembleome -- RSV Particles Form as Filamentous Structures at the Assembleome on Infected Cells -- The Assembleome Forms at Lipid Raft Microdomains on the Surface of Infected Cells. 327 $aFilamentous Actin (F-Actin) Remodeling at Lipid Raft Microdomains at the Assembleome Drives Virus Filament Formation -- Trafficking of the rac1 and rhoA Proteins to the Assembleome Is Required for Virus Filament Formation -- Future Perspectives -- References -- Chapter 10: Japanese Encephalitis Virus-Infected Cells -- Introduction -- Epidemiology -- Transmission Cycle -- Clinical Features -- JEV Molecular Biology -- Infection Route: A Cellular Overview -- Cellular Response to Infection -- Alteration of Signalling Pathways -- PKR Activation and Formation of Stress Granules -- Induction of Oxidative Stress -- Activation of the Unfolded Protein Response -- Upregulation of Autophagy -- Innate Immune Activation -- Cell Death Pathways -- Downregulation of Cell Adhesion Molecules -- Metabolic Reprogramming -- Infection Route: A Host Overview -- Infection Route in the Periphery -- Peripheral Immune Response to Infection -- Virus Entry into the CNS -- CNS Response to Infection -- Perspectives -- References -- Chapter 11: African Swine Fever Virus Host-Pathogen Interactions -- Introduction -- Virus Replication -- Virion Structure -- Virus Entry -- Early Steps in Replication -- Virus Factories -- Membranes -- Cytoskeleton -- Programmed Cell Death -- Autophagy -- Interferon Response -- Type I Interferons -- Induction of Type I Interferon Expression -- Interferon Regulatory Factors -- Nuclear Factor ?B -- Transmembrane Pathogen Recognition Receptors -- Cytosolic Pathogen Recognition Receptors -- IFN-Mediated Signalling -- ASFV Modulation of the IFN Response -- Inhibition of IFN Induction at the Level of Transcription Factors or Their Activation -- Inhibition of Specific PRRs Pathways -- Inhibition of IFN-Mediated Signalling -- Cellular Stress -- Secretory Pathway -- Concluding Remarks -- References. 327 $aChapter 12: Coronavirus and the Cytoskeleton of Virus-Infected Cells -- Introduction to the Cytoskeleton -- Cytoskeleton -- Actin Filaments -- Microtubules -- Intermediate Filaments -- Coronavirus -- The Structure and Life Cycle of Coronavirus -- SARS-CoV-2 and COVID-19 Infection -- Hazards of Other Virus Infections -- Response of the Host Cell Cytoskeleton Network Following Coronavirus Infection -- Coronavirus Infection Induces Expression Level or Posttranslational Modification Changes of the Cytoskeleton and its Related P... -- Coronavirus Infection Induces Actin Filament Rearrangement -- Microtubules Are Hijacked by Coronavirus for Trafficking -- Detection of Intermediate Filament Changes upon Infection -- Host Cytoskeleton Participates in Coronavirus Life Cycle -- Host Cytoskeleton Protein Facilitates Coronaviral Internalization -- Host Cytoskeleton Affects Coronaviral Replication -- Host Cytoskeleton Participates in the Coronaviral Assembly Process -- Coronaviral Release Can Be Promoted by Cytoskeleton Proteins -- Cytoskeleton and Pathogenesis -- The Ciliary Structure Is Involved in the Pathogenic Process -- Lung Injury Caused by SARS-CoV-2 Is Related to Endothelial Cell Permeability Regulation -- Relationship with the Nervous System -- Possibilities of Cytoskeleton-Related Treatment of COVID-19 -- Summary -- References -- Chapter 13: Viral RNA Is a Hub for Critical Host-Virus Interactions -- Introduction: RNA-Binding Proteins Are at the Core of Virus Infection -- RNA-Binding Proteins, Structure and Function -- The Expanding Universe of Cellular RNA-Binding Proteins -- The Essential Roles of Viral RBPs in Infection -- Virus Infection Profoundly Remodels the Cellular RNA-Binding Proteome (RBPome) -- How Are Cellular RBPs Regulated by a Virus Infection? -- New Methods to Uncover the Viral RNA Interactome. 327 $aMethods to Uncover the Viral RNA Interactome. 410 0$aSubcellular Biochemistry Series 606 $aBioquímica 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