LEADER 04770nam 2200625 450 001 9910797819403321 005 20230120002234.0 010 $a0-12-800030-9 010 $a0-12-800797-4 035 $a(CKB)3710000000527127 035 $a(EBL)4179308 035 $a(MiAaPQ)EBC4179308 035 $a(Au-PeEL)EBL4179308 035 $a(CaPaEBR)ebr11123674 035 $a(CaONFJC)MIL875733 035 $a(OCoLC)930489358 035 $a(EXLCZ)993710000000527127 100 $a20160102d2015 uy| 0 101 0 $aeng 135 $aur|n|---||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 00$aPlant cyclotides /$fvolume editor, David J. Craik, Institute for Molecular Bioscience, the University of Queensland, Brisbane, QLD, Australia 205 $aFirst edition. 210 1$aAmsterdam :$cElsevier,$d2015. 215 $a1 online resource (404 p.) 225 1 $aAdvances in botanical research,$x0065-2296 ;$vvolume seventy-six 300 $aDescription based upon print version of record. 320 $aIncludes bibliographical references and indexes. 327 $aFront Cover; ADVANCES IN BOTANICAL RESEARCH; Plant Cyclotides; Copyright; Contents; CONTRIBUTORS; PREFACE; One - Overview on the Discovery and Applications of Cyclotides; 1. INTRODUCTION; ACKNOWLEDGMENTS; REFERENCES; Two - Cyclotides in the Violaceae; 1. THE DISCOVERY OF CYCLOTIDES IN VIOLACEAE; 2. UNLOCKING THE CYCLOTIDE LIBRARY; 3. THE VIOLACEAE; 4. VIOLACEAE-THE PHARMACOPOEIA; 5. GROWING NEEDS AND SOURCES OF CYCLOTIDES; 6. COMPLETING THE CIRCLE; ACKNOWLEDGMENTS; REFERENCES; Three - Cyclotides in the Rubiaceae; 1. INTRODUCTION; 2. DISCOVERY OF CYCLOTIDES 327 $a2.1 Cyclotide Analytical Screening Workflow2.2 Cyclotides Sequence Analysis Using Transcriptomics and Peptidomics; 3. DISTRIBUTION OF CYCLOTIDES IN RUBIACEAE; 3.1 Rubiaceae-An Overview; 3.2 Morphology and Taxonomy of Rubiaceae; 3.3 Distribution of Cyclotides within Rubiaceae; 3.4 Cyclotide Precursors of Rubiaceae Plants; 3.5 Cyclotide Sequence Variations within Rubiaceae; 3.6 Distribution of Cyclotides in Other Gentianales; 4. EVOLUTION OF CYCLOTIDES; 5. BIOACTIVITIES OF RUBIACEAE CYCLOTIDES; 6. CONCLUSION AND OUTLOOK; ACKNOWLEDGMENTS; REFERENCES; Four - Cyclotides from Chinese Plants 327 $a1. Introduction2. Chemical Detection Method for Cyclotides; 3. Distribution and Chemotaxonomy of Cyclotides in Chinese Plants; 4. Extraction, Isolation and Structural Elucidation of Cyclotides in Chinese Plants; 4.1 Extraction and Isolation of Cyclotides in Chinese Plants; 4.2 Tandem MS Sequencing of Cyclotides in Chinese Plants; 4.3 NMR Characterization of Cyclotides in Chinese Plants; 5. Biological Activity of Cyclotides in Chinese Plants; Acknowledgments; References; Five - Primary Structural Analysis of Cyclotides; 1. CYCLOTIDE DISCOVERY-UNTANGLING THE CYSTINE KNOT 327 $a2. TOOLS FOR PEPTIDE SEQUENCE ASSIGNMENT2.1 Gene Sequencing; 2.2 Genome Mining; 2.3 Next-Generation Sequencing; 2.4 Peptide Extraction and Purification; 2.5 Acid Hydrolysis and Amino Acid Analysis; 2.6 Edman Degradation; 2.7 Chemical and Enzymatic Digestion; 3. MASS SPECTROMETRY; 3.1 Ionization; 3.1.1 Electrospray Ionization; 3.1.2 Nanoelectrospray Ionization; 3.1.3 Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry; 3.2 Mass Analyzers; 3.2.1 Quadrupole Mass Analyzers; 3.2.2 TOF Mass Analyzers; 3.3 Peptide Sequencing; 4. APPLICATION OF MASS SPECTROMETRY TO CYCLOTIDE SEQUENCING 327 $a4.1 MS Analysis of Cyclotides4.2 Tandem Mass Spectrometric (MS/MS) Analysis of Cyclotides; 4.3 Application of "Omics" Technologies to Cyclotide Sequencing; 5. CHALLENGES AND COMPLEXITIES; 5.1 Co-eluting, Isobaric Peptides; 5.2 N/D Isoforms; 5.3 Unknown Genomes/Incomplete DBs; 6. FUTURE PROSPECTS; 6.1 Transcriptomics and Bioinformatics; 6.2 Automation and Robotics; 6.3 Advances in MS Hardware; 6.4 Advances in MS Software; 7. CONCLUDING REMARKS; REFERENCES; Six - Structural Studies of Cyclotides; 1. THE CYCLIC CYSTINE KNOT; 2. STRUCTURAL FEATURES OF CYCLOTIDES; 2.1 Structural Studies 327 $a2.2 Mo?bius, Bracelets, and Trypsin Inhibitors 410 0$aAdvances in botanical research ;$vv. 76. 606 $aBotany$xResearch 606 $aPlant molecular biology 606 $aPlant proteomics 606 $aPeptides 606 $aBotanical chemistry 615 0$aBotany$xResearch. 615 0$aPlant molecular biology. 615 0$aPlant proteomics. 615 0$aPeptides. 615 0$aBotanical chemistry. 702 $aCraik$b David 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910797819403321 996 $aPlant cyclotides$93691044 997 $aUNINA