LEADER 03276oam 2200553I 450 001 9910797267703321 005 20230807215624.0 010 $a0-429-16192-1 010 $a1-4822-3557-9 024 7 $a10.1201/b18477 035 $a(CKB)3710000000413966 035 $a(EBL)1839844 035 $a(SSID)ssj0001542352 035 $a(PQKBManifestationID)16128996 035 $a(PQKBTitleCode)TC0001542352 035 $a(PQKBWorkID)14791684 035 $a(PQKB)10574923 035 $a(MiAaPQ)EBC1839844 035 $a(OCoLC)908931921 035 $a(EXLCZ)993710000000413966 100 $a20180331h20152015 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aCyclic nucleotide signaling /$fedited by Xiaodong Cheng 210 1$aBoca Raton, Florida :$cCRC Press,$d[2015] 210 4$dİ2015 215 $a1 online resource (282 p.) 225 1 $aMethods in Signal Transduction Series 300 $aDescription based upon print version of record. 311 $a1-4822-3556-0 320 $aIncludes bibliographical references at the end of each chapters. 327 $aFront Cover; Contents; Series Preface; Preface; Editor; Contributors; Chapter 1: Discovery of Small Molecule EPAC Specific Modulators by High-Throughput Screening; Chapter 2: Cyclic Nucleotide Analogs as Pharmacological Tools for Studying Signaling Pathways; Chapter 3: High-Throughput FRET Assays for Fast Time-Dependent Detection of Cyclic AMP in Pancreatic ? Cells; Chapter 4: Assessing Cyclic Nucleotide Recognition in Cells : Opportunities and Pitfalls for Selective Receptor Activation; Chapter 5: Monitoring Cyclic Nucleotides Using Genetically Encoded Fluorescent Reporters 327 $aChapter 6: Structural Characterization of Epac by X-Ray CrystallographyChapter 7: Sensory Neuron cAMP Signaling in Chronic Pain; Chapter 8: Monitoring Real-Time Cyclic Nucleotide Dynamics in Subcellular Microdomains; Chapter 9: Identifying Complexes of Adenylyl Cyclase with A-Kinase Anchoring Proteins; Chapter 10: Assessing Cyclic Nucleotide Binding Domain Allostery and Dynamics by NMR Spectroscopy; Chapter 11: A Protocol for Expression and Purification of Cyclic Nucleotide-Free Protein in Escherichia coli; Chapter 12: Cyclic Nucleotide Analogues as Chemical Tools for Interaction Analysis 327 $aChapter 13: Dissecting the Physiological Functions of PKA Using Genetically Modified MiceBack Cover 330 $a

Showcasing the recent progresses of the field, Cyclic Nucleotide Signaling covers the major tools and methodologies used in various areas of research. The majority of the chapters are protocol oriented, with the goal of providing clear directions for laboratory use. Students and investigators new to the field will find this book particularly informative, as will scientists already actively researching nucleotide signaling.

410 0$aMethods in signal transduction. 606 $aCyclic nucleotides 615 0$aCyclic nucleotides. 676 $a571.7/4 702 $aCheng$b Xiaodong 801 0$bFlBoTFG 801 1$bFlBoTFG 906 $aBOOK 912 $a9910797267703321 996 $aCyclic nucleotide signaling$93783577 997 $aUNINA