LEADER 05959nam 22007572 450 001 9910792700403321 005 20160429103422.0 010 $a1-107-21119-0 010 $a1-282-61995-0 010 $a9786612619953 010 $a0-511-72876-X 010 $a0-511-72781-X 010 $a0-511-72971-5 010 $a0-511-72642-2 010 $a0-511-73031-4 010 $a0-511-72500-0 035 $a(CKB)2670000000028801 035 $a(EBL)802917 035 $a(OCoLC)761647050 035 $a(SSID)ssj0000417655 035 $a(PQKBManifestationID)11292671 035 $a(PQKBTitleCode)TC0000417655 035 $a(PQKBWorkID)10369806 035 $a(PQKB)10104799 035 $a(UkCbUP)CR9780511730313 035 $a(Au-PeEL)EBL802917 035 $a(CaPaEBR)ebr10392903 035 $a(CaONFJC)MIL261995 035 $a(OCoLC)650374163 035 $a(MiAaPQ)EBC802917 035 $a(PPN)261297481 035 $a(EXLCZ)992670000000028801 100 $a20100308d2010|||| uy| 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aFemtosecond biophotonics $ecore technology and applications /$fMin Gu [and others]$b[electronic resource] 210 1$aCambridge :$cCambridge University Press,$d2010. 215 $a1 online resource (xii, 232 pages) $cdigital, PDF file(s) 300 $aTitle from publisher's bibliographic system (viewed on 05 Oct 2015). 311 $a0-521-88240-0 320 $aIncludes bibliographical references and index. 327 $aCover; Half-title; Title; Copyright; Dedication; Contents; Preface; 1 Introduction; 1.1 Femtosecond biophotonics; 1.2 Scope of the book; References; 2 Nonlinear optical microscopy; 2.1 Nonlinear optical microscopy; 2.1.1 Multi-photon fluorescence microscopy; 2.1.2 Harmonic generation microscopy; 2.1.3 Coherent anti-Stokes Raman scattering microscopy; 2.2 Two-photon fluorescence and second harmonic generation microscopy; 2.2.1 Comparison of single-photon and two-photon fluorescence imaging; 2.2.2 Reflection second harmonic generation microscopy through tissue 327 $a2.3 Three-dimensional two-photon autofluorescence spectroscopy2.4 Effect of handling and fixation processes on two-photon autofluorescence spectroscopy; 2.5 Two-photon excitation fluorescence resonance energy transfer; 2.6 Two-photon fluorescence lifetime imaging; References; 3 Two-photon fluorescence microscopy through turbid media; 3.1 Two-photon fluorescence microscopy of microspheres embedded in turbid media; 3.1.1 Measurement of two-photon fluorescence images; 3.1.2 Comparison with Monte-Carlo simulation; 3.2 Limiting factors on image quality in imaging through turbid media 327 $a3.3 Quantitative comparison of penetration depth between two-photon excitation and single-photon excitationReferences; 4 Fibre-optical nonlinear microscopy; 4.1 Fibre-optical confocal microscopy; 4.1.1 Image formation; 4.1.2 Milestones in fibre-optical confocal microscopy; 4.2 Two-photon fluorescence imaging systems using a single-mode optical fibre coupler; 4.2.1 Fibre-optical two-photon fluorescence microscopy; 4.2.2 Coupling efficiency and splitting ratio; 4.2.3 Spectral and temporal broadening; 4.2.4 Fluorescence axial response; 4.2.5 Three-dimensional optical transfer function analysis 327 $a4.2.6 Discussion4.3 Fibre-optical second harmonic generation microscopy; 4.3.1 Coupling efficiency and splitting ratio; 4.3.2 Second-harmonic generated axial response; 4.3.3 Three-dimensional coherent transfer function analysis; 4.3.4 Polarisation anisotropy; 4.4 Towards nonlinear endoscopic imaging; 4.5 Summary; References; 5 Nonlinear optical endoscopy; 5.1 An introduction to nonlinear optical endoscopy; 5.1.1 Optical fibres and ultrashort pulse delivery; 5.1.2 Scanning mechanisms; 5.1.3 Geometries of fibre-optical nonlinear optical microscopy 327 $a5.2 Nonlinear optical microscopy using double-clad PCFs5.2.1 Characterisation of double-clad PCFs; 5.2.2 Experimental arrangement; 5.2.3 Axial resolution; 5.2.4 Improvement of signal level; 5.2.5 Nonlinear optical imaging; 5.2.6 SHG polarisation anisotropy measurement; 5.3 A nonlinear optical endoscope based on a double-clad PCF and a MEMS mirror; 5.3.1 Endoscope design; 5.3.2 Axial resolution and signal level; 5.3.3 Endoscopic imaging; 5.3.4 3D tissue imaging; 5.4 Nonlinear optical microscopy using a double-clad PCF coupler; 5.4.1 A double-clad PCF coupler; 5.4.2 Experimental arrangement 327 $a5.4.3 System performance 330 $aThe introduction of femtosecond pulse lasers has provided numerous new methods for non-destructive diagnostic analysis of biological samples. This book is the first to provide a focused and systematic treatment of femtosecond biophotonic methods. Each chapter combines theory, practice and applications, walking the reader through imaging, manipulation and fabrication techniques. Beginning with an explanation of nonlinear and multiphoton microscopy, subsequent chapters address the techniques for optical trapping and the development of laser tweezers. In a conclusion that brings together the various topics of the book, the authors discuss the growing field of femtosecond micro-engineering. The wide range of applications for femtosecond biophotonics means this book will appeal to researchers and practitioners in the fields of biomedical engineering, biophysics, life sciences and medicine. 606 $aFemtosecond lasers 606 $aPhotonics 606 $aPhotobiology 615 0$aFemtosecond lasers. 615 0$aPhotonics. 615 0$aPhotobiology. 676 $a621.366 700 $aGu$b Min$f1960-$01516132 701 $aGu$b Min$f1960-$01516132 801 0$bUkCbUP 801 1$bUkCbUP 906 $aBOOK 912 $a9910792700403321 996 $aFemtosecond biophotonics$93752372 997 $aUNINA