LEADER 05520nam 2200745Ia 450 001 9910790326703321 005 20220622185405.0 010 $a1-283-57266-4 010 $a9786613885111 010 $a0-12-394833-9 035 $a(CKB)2670000000233489 035 $a(EBL)1001358 035 $a(OCoLC)810414726 035 $a(SSID)ssj0000737444 035 $a(PQKBManifestationID)11378476 035 $a(PQKBTitleCode)TC0000737444 035 $a(PQKBWorkID)10786598 035 $a(PQKB)10252491 035 $a(Au-PeEL)EBL1001358 035 $a(CaPaEBR)ebr10593898 035 $a(CaONFJC)MIL388511 035 $a(MiAaPQ)EBC1001358 035 $a(EXLCZ)992670000000233489 100 $a20111102h20122013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aForensic DNA biology$b[electronic resource] $ea laboratory manual /$fKelly M. Elkins 210 $aOxford $cElsevier$d2012, c2013 215 $a1 online resource (225 p.) 300 $aIncludes index. 311 $a0-12-394585-2 327 $aFront Cover; Forensic DNA Biology: A Laboratory Manual; Copyright; Contents; Acknowledgements; About the Author; Welcome; Forensic DNA Biology: An Introduction; BIOLOGY OVERVIEW; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; POLYMERASE CHAIN REACTION; SHORT TANDEM REPEATS; SINGLE NUCLEOTIDE POLYMORPHISMS; MITOCHONDRIAL DNA; KNOWN VERSUS QUESTIONED SAMPLES; WHY STUDY FORENSIC DNA BIOLOGY?; Laboratory Safety; RULES FOR A SAFE LAB ENVIRONMENT; Reference; Avoiding Contamination Issues: Standard Laboratory Practices; Reference; Chapter 1 - Pipetting; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND 327 $aPROCEDUREQUESTIONS; GRAPHING THE DATA USING MICROSOFT EXCEL (2003); EQUATIONS; References; Chapter 2 - Serology; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 3 - Sampling Biological Evidence for DNA Extraction; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE: DNA COLLECTION AND PACKAGING; QUESTIONS; References; Chapter 4 - DNA Extraction; OBJECTIVE; SAFETY; MATERIALS; RECIPES FOR BUFFER AND SOLUTION PREPARATION; BACKGROUND; PROCEDURE; QUESTION; References; Chapter 5 - Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis 327 $aOBJECTIVESAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; Reference; Chapter 6 - Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 7 - Determination of DNA Quantity by Fluorescence Spectroscopy; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 8 - Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References 327 $aChapter 9 - Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico)OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 10 - Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 11 - Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References 327 $aChapter 12 - Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex KitOBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTION; References; Chapter 13 - Computing Random Match Probability from DNA Profile Data Using Population Databases; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 14 - Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References 327 $aChapter 15 - Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit 330 $aDNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies w 606 $aForensic genetics$xTechnique 606 $aDNA fingerprinting 606 $aPolymerase chain reaction 606 $aDNA Fingerprinting 606 $aForensic Genetics$xmethods 606 $aPolymerase Chain Reaction 606 $aSequence Analysis, DNA$xmethods 615 0$aForensic genetics$xTechnique. 615 0$aDNA fingerprinting. 615 0$aPolymerase chain reaction. 615 2$aDNA Fingerprinting 615 2$aForensic Genetics$xmethods 615 2$aPolymerase Chain Reaction 615 2$aSequence Analysis, DNA$xmethods 676 $a572.86 676 $a614.1 700 $aElkins$b Kelly M$01139703 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910790326703321 996 $aForensic DNA biology$93674905 997 $aUNINA