LEADER 02289nam 2200553 a 450 001 9910789524503321 005 20230725032120.0 010 $a3-8428-0894-1 035 $a(CKB)2670000000106223 035 $a(EBL)741884 035 $a(OCoLC)745865861 035 $a(SSID)ssj0000949358 035 $a(PQKBManifestationID)11551459 035 $a(PQKBTitleCode)TC0000949358 035 $a(PQKBWorkID)10996993 035 $a(PQKB)10230261 035 $a(MiAaPQ)EBC741884 035 $a(Au-PeEL)EBL741884 035 $a(CaPaEBR)ebr10518937 035 $a(EXLCZ)992670000000106223 100 $a20120111d2011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aDesign of a single well bioreactor-incubator system with sterile nutrient media supply$b[electronic resource] /$fManuel Kohlmann 210 $aHamburg $cDiplomica Verlag$d2011 215 $a1 online resource (103 p.) 300 $aTitle from cover. 311 $a3-8428-5894-9 320 $aIncludes bibliographical references. 327 $aDesign of a Single Well Bioreactor-Incubator System with Sterile Nutrient Media Supply 330 $aHauptbeschreibungPerfused bioreactors have been developed with different designs and with various cell lines such as fibroblasts, osteoblasts and C2C12 myoblasts. All these current systems are custom made and mostly capable for only one cell type. Small seeding spaces and chambers inhibit longer cell studies and the observation of cell interaction. This study presents an advanced perfusion bioreactor which allows cell observation for longer than 5 days on an area of 2.8cm2 with a volume of 17ml. Initial experiments investigated homogeneity of the heat distribution, which is prec 606 $aBioreactors 606 $aIncubators 606 $aCulture media (Biology) 615 0$aBioreactors. 615 0$aIncubators. 615 0$aCulture media (Biology) 676 $a660/.6 700 $aKohlmann$b Manuel$01466568 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910789524503321 996 $aDesign of a single well bioreactor-incubator system with sterile nutrient media supply$93677065 997 $aUNINA