LEADER 00984nam a22002411i 4500 001 991000605829707536 005 20030227162346.0 008 021019s1982 it |||||||||||||||||ita 035 $ab12029063-39ule_inst 035 $aARCHE-011886$9ExL 040 $aDip.to Filologia Ling. e Lett.$bita$cA.t.i. Arché s.c.r.l. Pandora Sicilia s.r.l. 082 04$a470 245 00$aLingue e dialetti dell'Italia antica /$caggiornamenti e indici a cura di Anna Marinetti 260 $aPadova :$bIstituto di glottologia e fonetica dell'Università,$c1982 300 $a298 p. ;$c24 cm 650 4$aLingue e dialetti$xItalia antica 700 1 $aMarinetti, Anna 907 $a.b12029063$b02-04-14$c01-04-03 912 $a991000605829707536 945 $aLE008 Ist. Glott. X G 28$g1$i2008000469115$lle008$o-$pE0.00$q-$rl$s- $t0$u0$v0$w0$x0$y.i12319168$z01-04-03 996 $aLingue e dialetti dell'Italia antica$9142940 997 $aUNISALENTO 998 $ale008$b01-04-03$cm$da $e-$fita$git $h0$i1 LEADER 05331nam 22006734a 450 001 996213069903316 005 20230829010416.0 010 $a1-280-74882-6 010 $a9786610748822 010 $a0-470-76427-9 010 $a0-470-98887-8 010 $a1-4051-7307-6 035 $a(CKB)1000000000341810 035 $a(EBL)284294 035 $a(OCoLC)437176168 035 $a(SSID)ssj0000222365 035 $a(PQKBManifestationID)11187861 035 $a(PQKBTitleCode)TC0000222365 035 $a(PQKBWorkID)10171218 035 $a(PQKB)11000358 035 $a(MiAaPQ)EBC284294 035 $a(EXLCZ)991000000000341810 100 $a20060320d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aPlant proteomics$b[electronic resource] /$fedited by Christine Finnie 210 $aOxford, UK ;$aAmes, Iowa $cBlackwell Pub.$d2006 215 $a1 online resource (276 p.) 225 1 $aAnnual plant reviews ;$vv. 28 300 $aDescription based upon print version of record. 311 $a1-4051-4429-7 320 $aIncludes bibliographical references and index. 327 $aPlant Proteomics; Contents; Preface; Contributors; 1 Plant proteomics: challenges and resources; 1.1 Introduction; 1.2 Challenges; 1.2.1 Sample extraction; 1.2.1.1 Two-dimensional gel electrophoresis; 1.2.1.2 Direct MS analysis of samples; 1.2.2 Sample preparation and arraying; 1.2.2.1 Two-dimensional gel electrophoresis; 1.2.2.2 One-dimensional gel electrophoresis; 1.2.2.3 Blue-native gel electrophoresis; 1.2.2.4 Direct analysis of samples by MS; 1.2.3 Mass spectrometry (MALDI and ESI); 1.2.3.1 MALDI; 1.2.3.2 ESI; 1.2.4 Analysis depth; 1.2.5 Data analysis; 1.2.5.1 Peptide mass fingerprints 327 $a1.2.5.2 Peptide fragmentation data (MS/MS)1.2.5.3 Analysis options; 1.2.6 Quantitation; 1.2.6.1 Gel stains; 1.2.6.2 Chemical labelling of sample; 1.2.7 Modifications; 1.2.8 Data; 1.3 Resources; 1.3.1 Proteomic databases; 1.3.2 Online proteomic tools and resources; 1.4 Future; 2 Proteomic analysis of post-translational modifications by mass spectrometry; 2.1 Summary; 2.2 Introduction; 2.3 Considerations for the experimental design of PTM analysis by proteomics; 2.4 Analysis of PTMs by proteomic approaches; 2.4.1 Phosphorylation; 2.4.2 Protein glycosylation; 2.4.3 GPI-AP; 2.4.4 Farnesylation 327 $a2.4.5 N-terminally modified proteins2.5 Conclusions and perspectives; 3 Strategies for the investigation of protein-protein interactions in plants; 3.1 Summary; 3.2 Introduction; 3.3 Biochemical procedures to characterize protein-protein interactions; 3.3.1 Chromatographic purifications; 3.3.2 Sucrose gradient ultrafiltration; 3.3.3 Native gel electrophoresis; 3.3.4 Immunoprecipitations; 3.4 Genetic procedures to characterize protein-protein interactions; 3.4.1 Yeast two-hybrid system; 3.4.2 Yeast three-hybrid system; 3.4.3 Yeast one-hybrid system 327 $a3.4.4 Limitations of yeast two-hybrid systems3.4.5 Split-ubiquitin system; 3.4.6 Bimolecular fluorescence complementation (BiFC); 3.4.7 Fo?rster resonance energy transfer (FRET); 3.4.8 Tagging technologies for the purification of protein complexes; 3.5 Cytological procedures to characterize protein-protein interactions; 3.6 Outlook; 4 Proteomics of disulphide and cysteine oxidoreduction; 4.1 Introduction; 4.2 Control of cellular redox status; 4.2.1 Sequence and structural features of proteins catalysing cysteine redox modifications; 4.2.2 Catalytic mechanisms of Trxs and Grxs 327 $a4.3 Proteomics techniques for analysis of cysteine modifications4.3.1 Reagents for cysteine labelling; 4.3.2 Disulphide mapping; 4.3.3 S-glutathionylation; 4.3.4 Cysteine SOH, SO2H and SO3H; 4.3.5 Trxs and disulphide reduction; 4.3.6 S-nitrosylation; 4.4 Conclusions and perspectives; 5 Structural proteomics; 5.1 Introduction; 5.2 Project data handling: Sesame; 5.3 ORF cloning; 5.4 E. coli cell-based protein production pipeline; 5.4.1 Large-scale protein production and labeling; 5.4.2 Protein purification; 5.5 Wheat germ cell-free protein production 327 $a5.6 Mass spectrometry of purified proteins for quality assurance and analysis 330 $aThe proteome comprises all protein species resulting from gene expression in a cell, organelle, tissue or organism. By definition, proteomics aims to identify and characterise the expression pattern, cellular location, activity, regulation, post-translational modifications, molecular interactions, three dimensional structures and functions of each protein in a biological system.In plant science, the number of proteome studies is rapidly expanding after the completion of the Arabidopsis thaliana genome sequence, and proteome analyses of other important or emerging model systems and crop 410 0$aAnnual plant reviews ;$vv. 28. 606 $aPlant proteins 606 $aPlant proteomics 615 0$aPlant proteins. 615 0$aPlant proteomics. 676 $a572.62 676 $a572/.62 676 $a580.5 686 $a42.42$2bcl 701 $aFinnie$b Christine$0863228 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a996213069903316 996 $aPlant proteomics$91926951 997 $aUNISA LEADER 04914nam 22004813u 450 001 9910787358803321 005 20230124192940.0 010 $a92-2-124106-8 035 $a(CKB)3710000000347059 035 $a(EBL)1953119 035 $a(MiAaPQ)EBC1953119 035 $a(EXLCZ)993710000000347059 100 $a20150216d2012|||| u|| | 101 0 $aeng 135 $aur|n|---||||| 200 10$aMeasuring the Economically Active in Population Censuses$b[electronic resource] $eA Handbook 210 $aGeneva $cInternational Labour Office$d2012 215 $a1 online resource (353 p.) 300 $aDescription based upon print version of record. 311 $a92-2-124105-X 327 $aContents; Part One; General issues; Focus and content-; A. Overview; B. Purpose of Measuring the Economically Active; C. Users and uses of the Handbook; D. Organization of the Handbook; Planning and design of population; Uses and sources of statistics on economic characteristics; A. Uses of statistics on economic characteristics; B. Population census compared with other sources of data; Census administration from the perspective; C. General considerations in census planning and design; D. Preparation for enumeration; E. Decisions about the coverage and format of the enumeration 327 $a1. Scale of the census and use of sampling to expand content2. Issues relating to counts of population present and usual; 3. Method of census data collection; 4. Forms used for data collection; F. Selection, training and supervision of field staff; G. Field tests and the pilot census; H. Issues relating to questionnaire design for data processing; I. Data processing and dissemination of census results; Decisions related to topics for measuring; J. Selecting topics to be included; K. Determining the location and sequence of the economic; L. Formulating questions on selected topics; Part Two 327 $aMeasurement of economicMeasurement frameworks; A. Understanding the concept of economic activity; B. The scope of production in the System of National Accounts; C. Correspondence between economic activity and production; D. Decisions related to measuring economic characteristics; 1. Setting the reference period; 1.1. Period corresponding to current activity (labour force); 1.2. Period corresponding to usual activity; 1.3. The choice between current and usual activity reference periods; 2. Setting age limits; E. Organization of chapters IV to VIII; 1. Relevance of past experiences 327 $a2. Format of the presentationPart two; Part three; 2.1. Operational definition; 2.2. Requisites for applying the definition; 2.3. Review of questions used in national censuses; 2.4. Suggestions for enhancing the effectiveness of the questions; 2.5. Issues of measurement; Currently economically active; A. Labour force framework; B. Employed population; 1. Employed: operational definition; 2. Employed: requisites for applying the definition; 3. Identification of the employed: special cases; 4. Employed: review of questions used in national censuses 327 $a5. Employed: suggestions for enhancing the effectiveness of the questions6. Employed: issues of measurement; C. Unemployed population; 1. Unemployed: the operational definition; 2. Unemployed: requisites for applying the definition; 3. Unemployed: review of questions used in national censuses; 4. Unemployed: suggestions for enhancing the effectiveness; 5. Unemployed: issues of measurement; D. Population not currently active; Usual economic activity status; A. Introduction; B. Usually active: operational definition; C. Usually active: requisites for applying the definition 327 $aD. Usually active: review of questions used in national censuses 330 $aThis handbook provides guidance on the measurement of economic characteristics in population censuses, based on relevant experiences of countries, with a particular focus on the questions used and the requirements for processing of responses. It is intended to provide census planners with a variety of approaches to assess the questions and methods of collecting economic characteristics used in their national census, as they evaluate the performance in the past decade and plan for the 2010 round of censuses (2005-14). 606 $aCensus 606 $aPopulation -- Economic aspects -- Statistics -- Handbooks, manuals, etc 606 $aSocial science -- Demography 615 4$aCensus. 615 4$aPopulation -- Economic aspects -- Statistics -- Handbooks, manuals, etc. 615 4$aSocial science -- Demography. 676 $a304.60723 700 $aILO$01482496 701 $aNations$b United$0798177 801 0$bAU-PeEL 801 1$bAU-PeEL 801 2$bAU-PeEL 906 $aBOOK 912 $a9910787358803321 996 $aMeasuring the Economically Active in Population Censuses$93752630 997 $aUNINA