LEADER 05328nam 2200625Ia 450 001 9910787325803321 005 20230803030846.0 010 $a1-118-61927-7 010 $a1-118-61923-4 035 $a(CKB)2670000000400812 035 $a(EBL)1319494 035 $a(OCoLC)854521190 035 $a(SSID)ssj0001101554 035 $a(PQKBManifestationID)11650165 035 $a(PQKBTitleCode)TC0001101554 035 $a(PQKBWorkID)11067988 035 $a(PQKB)11043021 035 $a(MiAaPQ)EBC1319494 035 $a(Au-PeEL)EBL1319494 035 $a(CaPaEBR)ebr10738086 035 $a(CaONFJC)MIL527780 035 $a(EXLCZ)992670000000400812 100 $a20120904d2013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aUpstream industrial biotechnology$b[electronic resource] /$fedited by Michael C. Flickinger 210 $aHoboken, New Jersey $cJohn Wiley & Sons Inc.$dc2013 215 $a1 online resource (1838 p.) 300 $aDescription based upon print version of record. 311 $a1-118-13123-1 320 $aIncludes bibliographical references and index. 327 $aUPSTREAM INDUSTRIAL BIOTECHNOLOGY; CONTENTS; 27 Animal Cell Culture, Effects of Osmolality and Temperature; PREFACE; CONTRIBUTORS; PART I INTRODUCTION; PART II INDUSTRIAL CELL GROWTH AND GENE EXPRESSION SYSTEMS; 1 Animal Cells, Suspension Culture; 1.1 INTRODUCTION; 1.2 TYPES USED FOR LARGE-SCALE PRODUCTION IN SUSPENSION CULTURE; 1.3 SUSPENSION CULTURE REACTORS; 1.4 OPERATING MODES FOR REACTORS; 1.5 PROCESS MONITORING AND CONTROL; 1.6 CULTURE MEDIA FOR SUSPENSION CULTURE; 1.7 CONCLUSIONS; REFERENCES; 2 Baculovirus Expression Systems; 2.1 INTRODUCTION; 2.2 BACULOVIRUS STRUCTURE AND REPLICATION 327 $a2.3 PRODUCTION OF RECOMBINANT BACULOVIRUSES2.4 BACULOVIRUS TRANSFER VECTORS; 2.5 MODIFYING THE BACULOVIRUS GENOME TO IMPROVE PROTEIN PRODUCTION; 2.6 INSECT CELL CULTURE; 2.7 BACULOVIRUSES FOR GENE EXPRESSION IN MAMMALIAN CELLS; 2.8 CONCLUSION; REFERENCES; 3 Baculovirus Kinetics, Insect Culture; 3.1 HISTORY AND CHALLENGE; 3.2 BACULOVIRUS; 3.3 CELL YIELD CONCEPT; 3.4 KINETIC MODEL OF VIRAL INFECTION: SYNCHRONOUS INFECTION; 3.5 KINETIC MODEL OF VIRAL INFECTION: ASYNCHRONOUS INFECTION; REFERENCES; 4 Cell Culture, Aseptic Techniques; 4.1 INTRODUCTION; 4.2 ASEPTIC TECHNIQUE: GENERAL CONSIDERATIONS 327 $a4.3 ASEPTIC TECHNIQUE: BASIC PROCEDURES4.4 HEPA FILTRATION; 4.5 HOODS AND CABINETS EMPLOYING HEPA FILTRATION; 4.6 WORKING WITHIN UNIDIRECTIONAL AIRFLOW CABINETS AND MICROBIOLOGICAL SAFETY CABINETS; 4.7 TESTING OF CLASS I AND CLASS II MICROBIOLOGICAL SAFETY CABINETS; 4.8 CLEANROOMS FOR CELL CULTURE USE; REFERENCES; 5 Cell Cycle in Bioprocesses; 5.1 INTRODUCTION; 5.2 THE CELL CYCLE; 5.3 METHODS FOR DESCRIBING THE CELLCYCLE; 5.4 IMPORTANCE OF THE CELL CYCLE IN PROCESS BIOTECHNOLOGY; REFERENCES; 6 Cell Growth and Protein Expression Kinetics; 6.1 INTRODUCTION; 6.2 BATCH CULTURE KINETICS 327 $a6.3 CONTINUOUS CULTURE KINETICS6.4 FED-BATCH AND PERFUSION CULTURES; 6.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 7 Cell Viability Measurement; 7.1 INTRODUCTION; 7.2 PERMEABILITY ASSAYS; 7.3 FUNCTIONAL ASSAYS; 7.4 FLOW CYTOMETRY; 7.5 PHYSICAL METHODS; REFERENCES; 8 Contamination Detection in Animal Cell Culture; 8.1 INTRODUCTION; 8.2 HISTORICAL PERSPECTIVES; 8.3 REGULATORY ISSUES; 8.4 MANUFACTURING AND SAFETY TESTING STANDARDS; 8.5 EXAMPLES OF VIRAL CONTAMINANTS; 8.6 DETECTION OF VIRAL CONTAMINANTS IN CELL LINES; 8.7 TESTING RAW MATERIALS; 8.8 DETECTION OF MYCOPLASMAS; 8.9 BACTERIA AND FUNGI 327 $a8.10 OXYGEN UPTAKE RATE8.11 ENDOTOXIN DETECTION; 8.12 STATISTICAL ANALYSIS; 8.13 DETECTION OF PRIONS; 8.14 SUMMARY; REFERENCES; 9 Culture Collections and Biological Resource Centers (BRCs); 9.1 INTRODUCTION; 9.2 CULTURE COLLECTION FUNDING; 9.3 OPERATION; 9.4 QUALITY MANAGEMENT; 9.5 SERVICES; 9.6 SUMMARY; REFERENCES; FURTHER READING; 10 Culture Preservation; 10.1 INTRODUCTION; 10.2 CULTURE AND PRESERVATION OF BACTERIA; 10.3 CULTURE AND PRESERVATION OF FUNGI AND YEAST; 10.4 CULTURE AND PRESERVATION OF CELL CULTURES; REFERENCES 327 $a11 Expression and Secretion of Heterologous Proteins, Bacillus and Other Gram-Positive Bacteria 330 $aBiotechnology represents a major area of research focus, and many universities are developing academic programs in the field. This guide to biomanufacturing contains carefully selected articles from Wiley's Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology as well as new articles (80 in all,) and features the same breadth and quality of coverage and clarity of presentation found in the original. For instructors, advanced students, and those involved in regulatory compliance, this two-volume desk reference offers an accessible and comprehensive reso 606 $aBiotechnology 606 $aBiotechnology industries 615 0$aBiotechnology. 615 0$aBiotechnology industries. 676 $a660.6 686 $aSCI010000$2bisacsh 701 $aFlickinger$b Michael C$016579 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910787325803321 996 $aUpstream industrial biotechnology$93862831 997 $aUNINA LEADER 05467nam 2200745Ia 450 001 9910958134903321 005 20200520144314.0 010 $a9786613916525 010 $a9781118406595 010 $a1118406591 010 $a9781118406571 010 $a1118406575 010 $a9781283604079 010 $a1283604078 010 $a9781118406601 010 $a1118406605 035 $a(CKB)2670000000246169 035 $a(EBL)1022741 035 $a(OCoLC)798111149 035 $a(SSID)ssj0000711076 035 $a(PQKBManifestationID)11400281 035 $a(PQKBTitleCode)TC0000711076 035 $a(PQKBWorkID)10693309 035 $a(PQKB)11159994 035 $a(MiAaPQ)EBC1022741 035 $a(DLC) 2012026769 035 $a(Au-PeEL)EBL1022741 035 $a(CaPaEBR)ebr10602091 035 $a(CaONFJC)MIL391652 035 $a(Perlego)1004061 035 $a(EXLCZ)992670000000246169 100 $a20120628d2012 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aCareer planning for research bioscientists /$fSarah Blackford 205 $a1st ed. 210 $aHoboken, NJ $cWiley$d2012 215 $a1 online resource (194 p.) 300 $aDescription based upon print version of record. 311 08$a9781405196703 311 08$a140519670X 320 $aIncludes bibliographical references and index. 327 $aCareer Planning for Research Bioscientists; Copyright; Contents; Author's note; Acknowledgements; Chapter 1 Introduction; Who is this book for?; The process of career planning; Helping you with your career planning; Content of the book; How to use this book; References; Chapter 2 Planning your career; The importance of career planning; What is career planning?; Career planning in action; Conclusion; References; Chapter 3 Self-awareness; What is self-awareness?; Knowledge and interests; Skills; Personality; Values; Other contributing factors 327 $aPractical ways to analyse your 'self' and increase self-awareness1 Skills audit; 2 Personality assessment; 3 Values analysis; Conclusion; References; Chapter 4 The job market; Career sectors; Academic career path; Bioscience- or science-related careers; Careers outside science; Examples of job advertisements; (a) Postdoctoral posts; (b) Tenured academic posts; (c) Research in industry; (d) Science-related jobs in industry; (e) Science publishing and writing; (f) Administration; (g) Management programmes; Analysis of job advertisements; Research (academia); Research and technical (industry) 327 $aScience communicationAdministration; General management; Self-employment; Where are the jobs?; Accessing the 'visible' job market; Accessing the 'hidden' job market; Making the most of your network; Conclusion; References; Chapter 5 Enhancing your employability; The changing nature of work; Taking responsibility; Taking a proactive approach; Planned happenstance; Personal and professional development; Practise making funding applications; Attend conferences and meetings; Collaborate; Join/set up a group; Join professional organisations; Engage with social media; Mentoring; Teaching 327 $aSupervisionEntrepreneurship; Science communication; Voluntary work; Get support; Record your progress; Conclusion; References; Chapter 6 Making applications; Employer perspective; Presenting a professional image; Methods of application; Curriculum vitae; Application forms; Conclusion; Reference; Chapter 7 Successful interview technique; Types of interviews; One-to-one interviews; Telephone interviews; Skype interviews; Panel interviews; Presentations; Interview tasks; Assessment centres and psychometric testing; Interview content: what questions will you be asked? 327 $aJunior postdoctoral interviewsSenior postdoctoral researcher/group leader; Non-research posts; Answering the questions; Tackling the 'weaknesses' question; Thinking of questions to ask the interviewer; Preparation; If you are offered the job; If you are not offered the job; Conclusion; Reference; Chapter 8 Decision making and action planning; Careers in research; Other career options; Decision making; Decision-making styles; Taking action; Turning decisions into action; Writing a plan of action; Conclusion; References; Afterword; Appendix 1 Career narratives 327 $aSummarised list of career narratives 330 $a Career Planning for Research Bioscientists is an essential careers guide for bioscience doctoral students and postdoctoral researchers. It contains a wealth of information and resources specifically targeted at research bioscientists, with practical strategies to enhance career success in an increasingly competitive job market. Advice on how to write a winning CV together with examples adapted for different jobs is presented, as well as practical exercises to assist with skills analysis and decision making. Profiles of PhD-qualified bioscienstists in a range of professions including 606 $aBiology$xVocational guidance 606 $aCareer development 615 0$aBiology$xVocational guidance. 615 0$aCareer development. 676 $a570.23 700 $aBlackford$b Sarah$01803899 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910958134903321 996 $aCareer planning for research bioscientists$94351679 997 $aUNINA