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100   $a19890825d1990      uy             0
101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
182   $cc
183   $acr
200 10$aBiotechnology$b[electronic resource] $ea laboratory course /$fJeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo
210   $aSan Diego $cAcademic Press$dc1990
215   $a1 online resource (251 p.)
300   $aDescription based upon print version of record.
311   $a0-12-084560-1 
320   $aIncludes bibliographical references and index.
327   $aFront Cover; Biotechnology: A Laboratory Course; Copyright Page; Dedication; Table of Contents; Preface; Acknowledgments; Suggested Schedule for Exercises; Record Keeping and Safety Rules; Format of Student Laboratory Records; Ten Dos and Don'ts of Record Keeping; Criteria for Grading the Laboratory Notebook; Safety Rules in the Laboratory; Chapter 1. Measurement of pH; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 2. Use of Micropipettors and Spectrophotometers; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions
327   $aChapter 3.  Aseptic Technique: Transferring a CultureIntroduction; Reagents/Supplies; Equipment; Procedure; Questions; Chapter 4. Establishing a Pure Culture: The Streak Plate; Introduction; Reagents/Supplies; Equipment; Procedure; Questions; Chapter 5. Preparation of Culture Media; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 6. The Growth Curve; Introduction; Reagents/Supplies; Equipment; Procedure; Data Collection and Results; References; Questions; Chapter 7. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep; Introduction
327   $aReagents/SuppliesEquipment; Procedure; References; Questions; Chapter 8. Purification, Concentration, and Quantitation of DNA; Introduction; PART A PURIFICATION OF PLASMID DNA BY SOLVENT EXTRACTION; Reagents/Supplies; Equipment; Procedure; PART ? CONCENTRATION OF PLASMID DNA; Reagents/Supplies; Equipment; Procedure; PART C QUANTITATION OF DNA; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 9. Isolation of Plasmid DNA: The Maxi-Prep; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions
327   $aChapter 10. Restriction Digestion and Agarose Gel ElectrophoresisIntroduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 11. Southern Transfer; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 12. Preparation, Purification, and Hybridization of Probe; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 13. Transformation of Saccharomyces cerevisiae; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 14. Transformation of Escherichia coli by Plasmid DNA
327   $aIntroductionReagents/Supplies; Equipment; Procedure; References; Questions; Chapter 15. Protein Assays; Introduction; PART A LOWRY PROTEIN ASSAY; Reagents/Supplies; Equipment; Procedure; PART ? BRADFORD PROTEIN ASSAY; Reagents/Supplies; Equipment; Procedure; References; Question; Chapter 16. ?-Galaetosidase Assay; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 17. Determination of  ?-Galactosidase in Permeabilized Yeast Cells; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions
327   $aChapter 18. Assay of ?-Galactosidase in Cell Extracts
330   $aBiotechnology
606   $aBiotechnology$vLaboratory manuals
615  0$aBiotechnology
676   $a660.6
700   $aBecker$b Jeffrey M$0621784
701   $aCaldwell$b Guy A$01500534
701   $aZachgo$b Eve Ann$01500535
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910786464803321
996   $aBiotechnology$93727277
997   $aUNINA