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100   $a20040402d2004      uy             0
101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
182   $cc
183   $acr
200 00$aPhage display$b[electronic resource] $ea practical approach /$fedited by Tim Clackson, Henry B. Lowman
210   $aOxford ;$aNew York $cOxford University Press$dc2004
215   $a1 online resource (357 p.)
225 1 $aPractical approach series ;$vno. 266
300   $aDescription based upon print version of record.
311   $a0-19-963873-X 
311   $a0-19-963874-8 
320   $aIncludes bibliographical references and index.
327   $aContents; Protocol list; List of abbreviations; List of contributors; 1 Introduction to phage biology and phage display; 2 Constructing phage display libraries by oligonucleotide-directed mutagenesis; 3 In vitro DNA recombination; 4 Phage selection strategies for improved affinity and specificity of proteins and peptides; 5 Rapid screening of phage displayed protein binding affinities by phage ELISA; 6 Identification of novel ligands for receptors using recombinant peptide libraries; 7 Substrate phage display
327   $a8 Protease-based selection of stably folded proteins and protein domains from phage display libraries9 Phage display of zinc fingers and other nucleic acid-binding motifs; 10 In vivo and ex vivo selections using phage-displayed libraries; 11 Screening phage libraries with sera; 12 Interaction cloning using cDNA libraries displayed on phage; 13 Phage antibody libraries; 14 Affinity maturation of phage antibodies; Appendix: Non-standard suppliers; Index; 
330   $aThis new book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date,. comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using,
410  0$aPractical approach series ;$v266.
606   $aBacteriophages
606   $aViral proteins
606   $aMicrobial biotechnology
615  0$aBacteriophages.
615  0$aViral proteins.
615  0$aMicrobial biotechnology.
676   $a579.26
701   $aClackson$b Tim$01576469
701   $aLowman$b Henry B$01576470
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910784788703321
996   $aPhage display$93854297
997   $aUNINA