LEADER 05018nam 2200685Ia 450 001 9910784621503321 005 20210617162733.0 010 $a1-281-01881-3 010 $a9786611018818 010 $a0-08-054988-8 035 $a(CKB)1000000000383520 035 $a(EBL)307119 035 $a(OCoLC)476087167 035 $a(SSID)ssj0000175126 035 $a(PQKBManifestationID)11152251 035 $a(PQKBTitleCode)TC0000175126 035 $a(PQKBWorkID)10188766 035 $a(PQKB)10094534 035 $a(MiAaPQ)EBC307119 035 $a(Au-PeEL)EBL307119 035 $a(CaPaEBR)ebr10186627 035 $a(CaONFJC)MIL101881 035 $a(PPN)137604696 035 $a(EXLCZ)991000000000383520 100 $a20070307d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aHuman stem cell manual$b[electronic resource] $ea laboratory guide /$fedited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz 205 $a1st ed. 210 $aAmsterdam ;$aBoston $cElsevier/Academic Press$d2007 215 $a1 online resource (486 p.) 300 $aDescription based upon print version of record. 311 $a0-12-370465-0 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Human Stem Cell Manual; Copyright Page; Contents; Foreword by James Battey; Foreword by Jeannie Fontana; Preface by Jeanne Loring; Preface by Robin L. Wesselschmidt; Preface by Philip H. Schwartz; List of Contributors; Part I: Basic Methods in Stem Cell Culture; Chapter 1 Human Embryonic Stem Cell Culture; Chapter 2 Human Feeder Cells, Feeder-free, and Defined Culture Systems; Chapter 3 Mouse Embryonic Fibroblast Feeder Cells; Chapter 4 Cryopreservation of Human Embryonic Stem Cells; Part II: Characterization of Stem Cells; Chapter 5 Classical Cytogenetics: Karyotyping 327 $aChapter 6 Spectral Karyotyping and Fluorescent in situ HybridizationChapter 7 Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis; Chapter 8 Flow Cytometric Analysis of Human Embryonic Stem Cells; Chapter 9 Immunocytochemical Analysis of Stem Cells; Chapter 10 Characterization of Stem Cells Using Reverse Transcriptase Polymerase Chain Reaction; Chapter 11 Gene Expression Profiling of Stem Cells by Microarray; Chapter 12 Generation of Human Embryonic Stem Cell-derived Teratomas; Chapter 13 Characterization of Human Embryonic Stem Cell-derived Teratomas 327 $aPart III: Differentiation of Human Embryonic CellsChapter 14 Embryoid Bodies and Neuroepithelial Development; Chapter 15 Motor Neuron and Dopamine Neuron Differentiation; Chapter 16 Oligodendrocyte Differentiation from Human Embryonic Stem Cells; Chapter 17 Cardiac Development of Human Embryonic Stem Cells; Chapter 18 Hematopoiesis from Human Embryonic Stem Cells; Part IV: Genetic Manipulation of Stem Cells; Chapter 19 Genetic Manipulation of Human Embryonic Stem Cells: Lentivirus Vectors; Chapter 20 Genetic Manipulation of Embryonic Stem Cells; Part V: Advanced Methods 327 $aChapter 21 Derivation of Embryonic Stem Cells from Human BlastocystsChapter 22 Neural Progenitor Cell Culture; Chapter 23 Stem Cell Transplantation in the Brain; Chapter 24 In Vitro Fertilization; Chapter 25 Functional Characterization of Neurons; Part VI: Setting up a Laboratory; Chapter 26 Setting up a Facility for Human Embryonic Stem Cell Research; Part VII: Stem Cells and Society; Chapter 27 Intellectual Property: Owning the Stem Cell; Chapter 28 Ethical Concerns for Stem Cell Research; Chapter 29 Guidelines for Embryonic Stem Cell Research Oversight (ESCRO) Committees; Abbreviations 327 $aIndexA; B; C; D; E; F; G; H; I; K; L; M; N; O; P; Q; R; S; T; U; V; W; X; Y 330 $aStem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical ""hands on"" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practi 606 $aBiology$xResearch$xMethodology 606 $aEmbryonic stem cells 606 $aStem cells$vLaboratory manuals 615 0$aBiology$xResearch$xMethodology. 615 0$aEmbryonic stem cells. 615 0$aStem cells 676 $a616.02774 701 $aLoring$b Jeanne Frances$f1950-$01536949 701 $aSchwartz$b Philip H$01536950 701 $aWesselschmidt$b Robin L$01536951 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910784621503321 996 $aHuman stem cell manual$93786002 997 $aUNINA