LEADER 05334nam 22006494a 450 001 9910784562903321 005 20200520144314.0 010 $a1-281-02727-8 010 $a9786611027278 010 $a0-08-050061-7 035 $a(CKB)1000000000363597 035 $a(EBL)300592 035 $a(OCoLC)476078338 035 $a(SSID)ssj0000116786 035 $a(PQKBManifestationID)11145131 035 $a(PQKBTitleCode)TC0000116786 035 $a(PQKBWorkID)10036441 035 $a(PQKB)11226170 035 $a(Au-PeEL)EBL300592 035 $a(CaPaEBR)ebr10187211 035 $a(CaONFJC)MIL102727 035 $a(MiAaPQ)EBC300592 035 $a(PPN)182573524 035 $a(EXLCZ)991000000000363597 100 $a20030422d2003 uy 0 101 0 $aeng 135 $aurcn||||||||| 181 $ctxt 182 $cc 183 $acr 200 10$aCalculations in molecular biology and biotechnology$b[electronic resource] $ea guide to mathematics in the laboratory /$fFrank H. Stephenson 210 $aAmsterdam ;$aBoston $cAcademic Press$dc2003 215 $a1 online resource (319 p.) 300 $aIncludes index. 311 $a0-12-665751-3 327 $aFront Cover; Calculations in Molecular Biology and Biotechnology; Copyright Page; Contents; Foreword; Chapter 1. Scientific Notation and Metric Prefixes; Introduction; Significant Digits; Rounding Off Significant Digits in Calculations; Exponents and Scientific Notation; Expressing Numbers in Scientific Notation; Converting Numbers from Scientific Notation to Decimal Notation; Adding and Subtracting Numbers Written in Scientific Notation; Multiplying and Dividing Numbers Written in Scientific Notation; Metric Prefixes; Conversion Factors and Canceling Terms 327 $aChapter 2. Solutions Mixtures and Media Introduction; Calculating Dilutions: A General Approach; Concentrations by a Factor of X; Preparing Percent Solutions; Diluting Percent Solutions; Moles and Molecular Weight: Definitions; Molarity; Diluting Molar Solutions; Converting Molarity to Percent; Converting Percent to Molarity; Normality; PH; pKa and the Henderson-Hasselbalch Equation; Chapter 3. Cell Growth; The Bacterial Growth Curve; Manipulating Cell Concentration; Plotting OD550 vs. Time on a Linear Graph; Plotting the Logarithm of OD550 vs. Time on a Linear Graph 327 $aPlotting the Log of Cell Concentration vs. Time Calculating Generation Time; Plotting Cell Growth Data on a Semilog Graph; Determining Generation Time Directly from a Semilog Plot of Cell Concentration vs. Time; Plotting Cell Density versus OD550 on a Semilog Graph; The Fluctuation Test; Fluctuation Test Example; Variance; Measuring Mutation Rate; Measuring Cell Concentration on a Hemocytometer; Chapter 4. Working with Bacteriophage; Introduction; Multiplicity of Infection; Probabilities and Multiplicity of Infection; Measuring Phage Titer; Diluting Bacteriophage; Measuring Burst Size 327 $aChapter 5. Quantitation of Nucleic Acids Quantitation of Nucleic Acids by Ultraviolet Spectroscopy; Determining the Concentration of Double-Stranded DNA; Using Absorbance and an Extinction Coefficient to Calculate Double-Stranded DNA Concentration; Calculating DNA Concentration as a Millimolar (mM) Amount; Determining the Concentration of Single-Stranded DNA Molecules; Oligonucleotide Quantitation; Measuring RNA Concentration; Molecular Weight, Molarity, and Nucleic Acid Length; Estimating DNA Concentration on an Ethidium Bromide-Stained Gel 327 $aChapter 6. Labeling Nucleic Acids with Radioisotopes Introduction; Using Radioactivity: The Curie; Estimating Plasmid Copy Number; Labeling DNA by Nick Translation; Random Primer Labeling of DNA; Labeling 3' Termini with Terminal Transferase; cDNA Synthesis; Homopolymeric Tailing; In Vitro Transcription; Chapter 7. Oligonucleotide Synthesis; Introduction; Synthesis Yield; Measuring Stepwise and Overall Yield by the DMT Cation Assay; Overall Yield; Stepwise Yield; Calculating Micromoles of Nucleoside Added at Each Base Addition Step; Chapter 8. The Polymerase Chain Reaction; Introduction 327 $aTemplate and Amplification 330 $aCalculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. Mathematics, as a vital component of the successful design and interpretation of basic research, is used daily in laboratory work. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow 606 $aMolecular biology$xComputer simulation 606 $aMolecular biology$xMathematics 606 $aBiotechnology 615 0$aMolecular biology$xComputer simulation. 615 0$aMolecular biology$xMathematics. 615 0$aBiotechnology. 676 $a572.8/01/51 700 $aStephenson$b Frank Harold$0861236 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910784562903321 996 $aCalculations in molecular biology and biotechnology$93708341 997 $aUNINA