LEADER 05482nam 2200673Ia 450 001 9910784528503321 005 20200520144314.0 010 $a1-281-27948-X 010 $a9786611279486 010 $a0-08-052809-0 035 $a(CKB)1000000000384303 035 $a(EBL)340619 035 $a(OCoLC)437208893 035 $a(SSID)ssj0000112165 035 $a(PQKBManifestationID)11134233 035 $a(PQKBTitleCode)TC0000112165 035 $a(PQKBWorkID)10087185 035 $a(PQKB)11336201 035 $a(Au-PeEL)EBL340619 035 $a(CaPaEBR)ebr10254644 035 $a(CaONFJC)MIL127948 035 $a(MiAaPQ)EBC340619 035 $a(PPN)182571645 035 $a(EXLCZ)991000000000384303 100 $a19980407d1999 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBiomedical electron microscopy$b[electronic resource] $eillustrated methods and interpretations /$fArvid B. Maunsbach, Bjo?rn A. Afzelius 210 $aSan Diego, CA $cAcademic Press$dc1999 215 $a1 online resource (569 p.) 300 $aDescription based upon print version of record. 311 $a0-12-480610-4 320 $aIncludes bibliographical references and index. 327 $aFront Cover; BIOMEDICAL ELECTRON MICROSCOPY; Copright Page; FOREWORD; PREFACE; ACKNOWLEDGMENTS; CHAPTER 1. MICROGRAPH INTERPRETATION; 1. Classical Preparation Method; 2. Low Temperature Approach; 3. A Common Test Specimen; 4. Detection of Objects; 5. Identification of Artifacts; 6. Analysis of Geometry; 7. Biological Identification; 8. Biological Diversity; 9. Analysis of Dynamics: Endocytosis; 10. Analysis of Dynamics: Synthesis; 11. Comparison of Methods; 12. Variations in Magnifications; 13. Interpretation Difficulties; 14. Diagnostic Pathology; CHAPTER 2. FIXATIVES 327 $a1. Osmium Tetroxide and Glutaraldehyde at Low Magnification2. Osmium Tetroxide and Glutaraldehyde at High Magnification; 3. Glutaraldehyde Concentration: Perfusion Fixation; 4. Glutaraldehyde Concentration: Immersion Fixation; 5. Long Fixation Times; 6. Formaldehyde-Glutaraldehyde Combinations; 7. Potassium Permanganate, Picric Acid, and Ruthenium Red; 8. Lead Salts and Tannic Acid; 9. Uranyl Acetate Postfixation; 10. Tannic Acid-Uranyl Acetate Variations; 11. Osmium Tetroxide-Potassium Ferrocyanide; 12. Osmium Tetroxide Artifacts; 13. Glutaraldehyde Artifacts; CHAPTER 3. FIXATIVE VEHICLE 327 $a1. Absence and Presence of Buffer2. Comparison of Buffers; 3. Osmolality of Perfusion Fixatives; 4. Effects of Osmolality on Cell Shape; 5. Effects of Osmolality on Cell Organelles; 6. Adjustment of Osmolality with Sucrose; 7. Colloid Osmotic Pressure: Low Magnification; 8. Colloid Osmotic Pressure: High Magnification; 9. Phosphate Buffer Precipitate; CHAPTER 4. FIXATIVE APPLICATION; 1. Perfusion-Fixation versus Immersion-Fixation; 2. Perfusion-Fixation with Pressure Control; 3. Fixation by Dripping in Vivo; 4. Immersion-Fixation; 5. Variability within the Tissue 327 $a6. Unsuccessful Perfusion-Fixation7. Superficial Tissue Damage; 8. Early Postmortal Changes; 9. Late Postmortal Changes; 10. Influence of Biopsy Method; 11. Microwave Treatment; CHAPTER 5. DEHYDRATION AND EMBEDDING; 1. Stepwise versus Direct Dehydration; 2. Prolonged Dehydration in Ethanol; 3. Prolonged Dehydration in Acetone; 4. Inert Dehydration; 5. Choice of Intermediate Solvent; 6. Epon, Araldite, and Vestopal: Unstained Sections; 7. Epon, Araldite, and Vestopal: Stained Sections; 8. Different Brands of Epoxy Resins; 9. Spurr and LR White; 10. Embedding of Isolated Cells 327 $aCHAPTER 6. FREEZING AND LOW-TEMPERATURE EMBEDDING1. Plunge Freezing; 2. Contact Freezing of Unfixed Tissue; 3. Contact Freezing of Fixed Tissue; 4. High-Pressure Freezing; 5. Freeze-Substitution in Methanol/Uranyl Acetate; 6. Freeze-Substitution in Osmium Tetroxide Acetone; 7. Progressive Lowering of Temperature Embedding in Lowicryl; CHAPTER 7. SUPPORT FILMS; 1. Surface Topography; 2. Stability of Film or Section; 3. Holey Films; 4. Thick and Thin Support Films; 5. Folds in Support Film; 6. Defects in Formvar Films; 7. Common Contaminants; 8. Volatile Contamination; CHAPTER 8. ULTRAMICROTOMY 327 $a1. Correlation of Light and Electron Microscopy 330 $aThis comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.Key Features* Authored by the key leaders in the biological electron microscopy field* Illustrates both optimal 606 $aElectron microscopy$xTechnique 606 $aMedical microscopy$xTechnique 615 0$aElectron microscopy$xTechnique. 615 0$aMedical microscopy$xTechnique. 676 $a502.825 676 $a570.2825 676 $a570/.28/25 21 700 $aMaunsbach$b Arvid Bernhard$01545405 701 $aAfzelius$b Bjo?rn$0353250 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910784528503321 996 $aBiomedical electron microscopy$93800319 997 $aUNINA