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100   $a20050504d2006      uy             0
101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
182   $cc
183   $acr
200 12$aA practical handbook of preparative HPLC$b[electronic resource] /$fDonald A. Wellings
210   $aOxford $cElsevier$d2006
215   $a1 online resource (193 p.)
300   $aDescription based upon print version of record.
311   $a1-85617-466-2 
320   $aIncludes bibliographical references and index.
327   $aCover; copyright; Contents; front matter; Preface; Foreword; Abbreviations; body; 1 The history and development of preparative HPLC; 2 Fluid dynamics, mass transport and friction; 3 Modes of chromatographic separation; Particle size; Pore structure, size and surface area; 3.1 Normal phase chromatography; 3.2 Reversed phase chromatography; 3.3 Chiral separations; Chirality; Optical purity; Separation of enantiomers; 3.4 Ion exchange chromatography; 3.5 Exclusion chromatography; 3.6 Affinity chromatography; 4 How to get started; 4.1 Packing a column; Column packing protocol for column type 1
327   $aSlurry concentrationSlurry and column preparation; Column packing protocol for column type 2; 4.2 What and where!; 4.3 Product recovery; 4.4 Productivity; 5 Process development and optimization; 5.1 Sample self displacement for purification of a peptide; Analytical HPLC; Gradient selection; Loading study; Flow rate optimization; 5.2 Boxcar injections for chiral separations; 6 Documentation and record keeping; 6.1 Equipment qualification; 6.2 Process documentation; back matter; Appendices; PROCESS INSTRUCTIONS; CONTENTS; DEVIATION SHEET; OPERATION 1 PREPARATION SECTION; 1.01 Abbreviations
327   $a1.02 Target Specifications1.03 Raw Materials; 1.04 Equipment; 1.05 Consumables; 1.06 In-Process Tests; Analytical HPLC Conditions; Analytical HPLC Strength Method; 1.07 Ancillary SOPs; 1.08 Cleaning; 1.09 Personnel and Responsibilities; 1.10 Safety; Safety Precautions; OPERATION 2 PRE-PURIFICATION COLUMN PREPARATION; OPERATION 3 PURIFICATION; PURIFICATION DEVIATION SHEET; EXAMPLE OF FRACTION RECONCILIATION DOCUMENT; OPERATION 1 SUMMARY; References; CHAPTER 1; CHAPTER 2; CHAPTER 3; CHAPTER 4; CHAPTER 5; CHAPTER 6; Index
330   $aThis book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.This book...Provides practical, hands-on advice based on years of experienceWill help ensure optimal design,
606   $aHigh performance liquid chromatography
606   $aLiquid chromatography
615  0$aHigh performance liquid chromatography.
615  0$aLiquid chromatography.
676   $a543.84
700   $aWellings$b Donald A$01543587
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910784017303321
996   $aA practical handbook of preparative HPLC$93797140
997   $aUNINA