LEADER 04320nam 2200661 a 450 001 9910782096403321 005 20200520144314.0 010 $a1-281-13990-4 010 $a9786611139902 010 $a0-387-72972-0 024 7 $a10.1007/978-0-387-72972-5 035 $a(CKB)1000000000492334 035 $a(EBL)337828 035 $a(OCoLC)437206576 035 $a(SSID)ssj0000111962 035 $a(PQKBManifestationID)11132998 035 $a(PQKBTitleCode)TC0000111962 035 $a(PQKBWorkID)10081229 035 $a(PQKB)10870134 035 $a(DE-He213)978-0-387-72972-5 035 $a(MiAaPQ)EBC337828 035 $a(Au-PeEL)EBL337828 035 $a(CaPaEBR)ebr10217696 035 $a(CaONFJC)MIL113990 035 $a(PPN)123736773 035 $a(EXLCZ)991000000000492334 100 $a20070625d2008 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aBiological low-voltage scanning electron microscopy$b[electronic resource] /$fedited by Heide Schatten, James B. Pawley 205 $a1st ed. 2008. 210 $aNew York $cSpringer$dc2008 215 $a1 online resource (328 p.) 300 $aDescription based upon print version of record. 311 $a1-4899-9584-6 311 $a0-387-72970-4 320 $aIncludes bibliographical references and index. 327 $aThe Early Development of the Scanning Electron Microscope -- LVSEM for Biology -- The Aberration-Corrected SEM -- Noise and Its Effects on the Low-Voltage SEM -- High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols -- Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM -- Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM -- Cryo-SEM of Chemically Fixed Animal Cells -- High-Resolution and Low-Voltage SEM of Plant Cells -- High-Resolution Cryoscanning Electron Microscopy of Biological Samples -- Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy. 330 $aField-emission, low-voltage scanning electron microscopy (LVSEM) is a field that has grown tremendously in recent years because is offers the optimal method for viewing complex surfaces at high resolution and in three dimensions. However, even though the instrumentation required to get good results at low beam voltage has become increasingly available, there has been a lag in its application to biological specimens. What seemed to be missing was volume that combined both the theory and practice of using this equipment in an optimal manner with a thorough treatment of biological specimen preparation. Biological Low-Voltage Scanning Electron Microscopy is the first book to address both of these aspects of biological LVSEM. After providing a thorough description of the unique advantages and the operating constraints related to operating a scanning electron microscope at low beam voltage, the remainder of book focuses on the the best way to image all types of plant and animal cells and covers specimens that range from macromolecules to the surfaces revealed by de-embedding resin-embedded samples. Advanced specimen preparation techniques such as cryo-LVSEM, and immuno-gold-LVSEM are fully covered, as is x-ray microanalysis at low beam voltage and live-time stereo imaging. The preparative protocols provided represent the distilled essence of the experience of a group of world-renowned authors who have, for many decades, been instrumental in developing and applying new approaches to LVSEM to support their own biological research. 606 $aLow-voltage scanning electron microscopy 606 $aField emission cathodes 606 $aScanning electron microscopes 615 0$aLow-voltage scanning electron microscopy. 615 0$aField emission cathodes. 615 0$aScanning electron microscopes. 676 $a570.2825 701 $aSchatten$b Heide$0899576 701 $aPawley$b James B$0306051 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910782096403321 996 $aBiological low-voltage scanning electron microscopy$93680711 997 $aUNINA