LEADER 04167nam 2200661Ia 450 001 9910781937603321 005 20230713120420.0 010 $a1-283-31097-X 010 $a9786613310972 010 $a0-12-385545-4 035 $a(CKB)2550000000063471 035 $a(EBL)802466 035 $a(OCoLC)769342081 035 $a(SSID)ssj0000591978 035 $a(PQKBManifestationID)11352048 035 $a(PQKBTitleCode)TC0000591978 035 $a(PQKBWorkID)10728666 035 $a(PQKB)11568203 035 $a(Au-PeEL)EBL802466 035 $a(CaPaEBR)ebr10509200 035 $a(CaONFJC)MIL331097 035 $z(PPN)182565076 035 $a(MiAaPQ)EBC802466 035 $a(PPN)176592040 035 $a(EXLCZ)992550000000063471 100 $a20111109d2012 uy 0 101 0 $aeng 135 $aurcn||||||||| 181 $ctxt$2rdacontent 181 $csti$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aMolecular biology techniques$b[electronic resource] $ea classroom laboratory manual /$fSusan Carson, Heather Miller, D. Scott Witherow 205 $a3rd ed. 210 $aAmsterdam ;$aBoston $cElsevier /Academic Press$dc2012 215 $a1 online resource (xxvi, 200 pages) $ccolor illustrations 300 $aIncludes index. 300 $aPrevious edition: 2006. 311 $a0-12-385544-6 320 $aIncludes bibliographical references and index. 327 $aLab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions. 330 $aThis manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, 606 $aMolecular biology$vLaboratory manuals 606 $aMolecular biology$xTechnique$vHandmooks, manuals, etc 615 0$aMolecular biology 615 0$aMolecular biology$xTechnique 676 $a572.8/078 676 $a572.8078 700 $aCarson$b Sue$cDr.$01527848 701 $aMiller$b Heather B$01527849 701 $aWitherow$b D. Scott$g(Dennis Scott)$01527850 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910781937603321 996 $aMolecular biology techniques$93771109 997 $aUNINA