LEADER 05590nam 2200685 450 001 9910781098103321 005 20230125234323.0 010 $a1-5231-4634-6 010 $a1-59693-403-4 035 $a(CKB)2550000000001247 035 $a(EBL)946540 035 $a(OCoLC)796382983 035 $a(SSID)ssj0000343249 035 $a(PQKBManifestationID)11252686 035 $a(PQKBTitleCode)TC0000343249 035 $a(PQKBWorkID)10290971 035 $a(PQKB)10023895 035 $a(Au-PeEL)EBL946540 035 $a(CaPaEBR)ebr10359051 035 $a(CaBNVSL)mat09100720 035 $a(IEEE)9100720 035 $a(MiAaPQ)EBC946540 035 $a(EXLCZ)992550000000001247 100 $a20200729d2009 uy 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aStem cell bioengineering /$fBiju Parekkadan, Martin L. Yarmush, editors 210 1$aBoston, Massachusetts ;$cArtech House,$dİ2009. 210 2$a[Piscataqay, New Jersey] :$cIEEE Xplore,$d[2009] 215 $a1 online resource (222 p.) 225 1 $aMethods in bioengineering series 300 $aSeries title given on title page as: Methods in bioengineering. 311 $a1-59693-402-6 320 $aIncludes bibliographical references and index. 327 $aMethods in Bioengineering: Stem Cell Bioengineering; Contents; Preface; Chapter 1 Somatic Cell Nuclear Transfer and Derivation of Embryonic Stem Cells; 1.1 Introduction; 1.2 Materials for Nuclear Transfer; 1.2.1 Equipment for mouse nuclear transfer; 1.2.2 Reagents for mouse nuclear transfer; 1.3 Methods for Nuclear Transfer; 1.3.1 Preparation of enucleation and nuclear transfer pipettes; 1.3.2 Medium preparation; 1.3.3 Animal preparation; 1.3.4 Nuclear transfer; 1.3.5 Enucleation; 1.3.6 Preparation of donor cells; 1.3.7 Nuclear transfer; 1.3.8 Activation 327 $a1.3.9 Embryo culture and embryo transfer1.4 Derivation of Mouse ntES Cells; 1.5 Materials for Embryonic Stem Cell Derivation; 1.6 Methods for Embryonic Stem Cell Derivation; 1.6.1 Derivation of ntES cells; 1.6.2 In vitro characterization of ntES cells; 1.6.3 In vivo characterization of ntES cells; 1.7 Discussion and Commentary; 1.8 Summary Points; Acknowledgments; References; Chapter 2 Derivation of Mouse Parthenogenetic Embryonic Stem Cells; 2.1 Introduction; 2.2 Materials; 2.2.1 Reagents; 2.2.2 Equipment; 2.2.3 Media recipe; 2.3 Methods; 2.3.1 Generation of p(MI) embryos 327 $a2.3.2 Generation of p(MII) embryos2.3.3 Generation of p(hap) embryos; 2.3.4 Derivation of p(MI), p(MII), and p(hap) ES cells; 2.3.5 ES cell characterization; 2.3.6 Teratoma induction; 2.4 Data Acquisition, Anticipated Results, and Interpretation; 2.5 Discussion and Commentary; 2.6 Summary Points; Acknowledgments; References; Chapter 3 Generation of Mice from Embryonic Stem Cells Using Tetraploid Embryos as Hosts; 3.1 Introduction; 3.2 Experimental Design; 3.3 Materials; 3.3.1 Preparation of 4n embryos: Mice and reagents; 3.3.2 Preparation of 4n embryos: Equipment 327 $a3.3.3 ES cells and culture conditions3.3.4 Micromanipulation system; 3.4 Methods; 3.4.1 Preparation of host embryos; 3.4.2 Electrofusion of two-cell stage embryos; 3.4.3 Removal of the zona pellucida; 3.4.4 Generation of ES multiple 4n embryos by aggregation; 3.4.5 Generation of ES 4n embryos by blastocyst injection; 3.4.6 Data acquisition; 3.5 Anticipated Results; 3.6 Discussion and Commentary; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter4 Bioreactor Design and Implementation; 4.1 Introduction; 4.2 Experimental Methods and Materials 327 $a4.2.1 General system description4.2.2 Closed system; 4.2.3 Hollow-fiber bioreactor; 4.2.4 CES fluid circuit; 4.2.5 Oxygenator design; 4.2.6 Monitoring; 4.2.7 User interface; 4.3 Anticipated Results; 4.3.1 MSC source 1: Loading whole BM marrow into the CES; 4.3.2 MSC source 2 or 3: Loading preselected MSC into the CES; 4.4 Discussion and Commentary; 4.5 Application Notes; 4.5.1 Therapeutic dose of MSC grown from a whole BM sample; 4.5.2 Nonadherent cell culture: Kg1a cells grown in suspension; 4.6 Summary Points; Chapter 5 Extracellular Matrix Microarrays and Stem Cell Fate; 5.1 Introduction 330 3 $aWritten and edited by recognized experts in the field, the new Artech House Methods in Bioengineering book series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This cutting-edge volume is focused on methods to derive, manipulate, target, and/or prepare stem cells for clinical use. The book helps you master powerful stem cell bioengineering methods, enabling them to rigorously test hypotheses and compare their results to "gold standards".$cPublisher abstract. 410 0$aArtech House methods in bioengineering series. 606 $aBioengineering 606 $aStem cells$xResearch 606 $aStem cells 615 0$aBioengineering. 615 0$aStem cells$xResearch. 615 0$aStem cells. 676 $a616.02774 701 $aParekkadan$b Biju$01568873 701 $aYarmush$b Martin L$01568874 801 0$bCaBNVSL 801 1$bCaBNVSL 801 2$bCaBNVSL 906 $aBOOK 912 $a9910781098103321 996 $aStem cell bioengineering$93841309 997 $aUNINA