LEADER 05316nam 22006494a 450 001 9910780374003321 005 20230617011832.0 010 $a1-280-36794-6 010 $a9786610367948 010 $a0-470-23229-3 010 $a0-471-62386-5 010 $a0-471-62362-8 035 $a(CKB)111087027142166 035 $a(EBL)468856 035 $a(OCoLC)54711493 035 $a(SSID)ssj0000111573 035 $a(PQKBManifestationID)11145479 035 $a(PQKBTitleCode)TC0000111573 035 $a(PQKBWorkID)10080713 035 $a(PQKB)11551225 035 $a(DNLM)101185860 035 $a(MiAaPQ)EBC468856 035 $a(Au-PeEL)EBL468856 035 $a(CaPaEBR)ebr10114042 035 $a(CaONFJC)MIL36794 035 $a(EXLCZ)99111087027142166 100 $a20030723d2004 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBioanalytical chemistry$b[electronic resource] /$fSusan R. Mikkelsen, Eduardo Corto?n 210 $aHoboken, N.J. $cJohn Wiley & Sons$dc2004 215 $a1 online resource (381 p.) 300 $aDescription based upon print version of record. 311 $a0-471-54447-7 320 $aIncludes bibliographical references and index. 327 $aBIOANALYTICAL CHEMISTRY; CONTENTS; Preface; Acknowledgments; 1. Spectroscopic Methods for Matrix Characterization; 1.1 Introduction; 1.2 Total Protein; 1.2.1 Lowry Method; 1.2.2 Smith (BCA) Method; 1.2.3 Bradford Method; 1.2.4 Ninhydrin-Based Assay; 1.2.5 Other Protein Quantitation Methods; 1.3 Total DNA; 1.3.1 Diaminobenzoic Acid Method; 1.3.2 Diphenylamine Method; 1.3.3 Other Fluorometric Methods; 1.4 Total RNA; 1.5 Total Carbohydrate; 1.5.1 Ferricyanide Method; 1.5.2 Phenol-Sulfuric Acid Method; 1.5.3 2-Aminothiophenol Method; 1.5.4 Purpald Assay for Bacterial Polysaccharides 327 $a1.6 Free Fatty AcidsReferences; Problems; 2. Enzymes; 2.1 Introduction; 2.2 Enzyme Nomenclature; 2.3 Enzyme Commission Numbers; 2.4 Enzymes in Bioanalytical Chemistry; 2.5 Enzyme Kinetics; 2.5.1 Simple One-Substrate Enzyme Kinetics; 2.5.2 Experimental Determination of Michaelis-Menten Parameters; 2.5.2.1 Eadie-Hofstee Method; 2.5.2.2 Hanes Method; 2.5.2.3 Lineweaver-Burk Method; 2.5.2.4 Cornish-Bowden-Eisenthal Method; 2.5.3 Comparison of Methods for the Determination of K(m) Values; 2.5.4 One-Substrate, Two-Product Enzyme Kinetics; 2.5.5 Two-Substrate Enzyme Kinetics 327 $a2.5.6 Examples of Enzyme-Catalyzed Reactions and Their Treatment2.6 Enzyme Activators; 2.7 Enzyme Inhibitors; 2.7.1 Competitive Inhibition; 2.7.2 Noncompetitive Inhibition; 2.7.3 Uncompetitive Inhibition; 2.8 Enzyme Units and Concentrations; Suggested References; References; Problems; 3. Quantitation of Enzymes and Their Substrates; 3.1 Introduction; 3.2 Substrate Depletion or Product Accumulation; 3.3 Direct and Coupled Measurements; 3.4 Classification of Methods; 3.5 Instrumental Methods; 3.5.1 Optical Detection; 3.5.1.1 Absorbance; 3.5.1.2 Fluorescence; 3.5.1.3 Luminescence 327 $a3.5.1.4 Nephelometry3.5.2 Electrochemical Detection; 3.5.2.1 Amperometry; 3.5.2.2 Potentiometry; 3.5.2.3 Conductimetry; 3.5.3 Other Detection Methods; 3.5.3.1 Radiochemical; 3.5.3.2 Manometry; 3.5.3.3 Calorimetry; 3.6 Ultra-High-Throughput Assays (HTA); 3.7 Practical Considerations for Enzymatic Assays; Suggested References; References; Problems; 4. Immobilized Enzymes; 4.1 Introduction; 4.2 Immobilization Methods; 4.2.1 Nonpolymerizing Covalent Immobilization; 4.2.1.1 Controlled-Pore Glass; 4.2.1.2 Polysaccharides; 4.2.1.3 Polyacrylamide; 4.2.1.4 Acidic Supports; 4.2.1.5 Anhydride Groups 327 $a4.2.1.6 Thiol Groups4.2.2 Cross-Linking with Bifunctional Reagents; 4.2.3 Adsorption; 4.2.4 Entrapment; 4.2.5 Microencapsulation; 4.3 Properties of Immobilized Enzymes; 4.4 Immobilized Enzyme Reactors; 4.5 Theoretical Treatment of Packed-Bed Enzyme Reactors; Suggested References; References; Problems; 5. Antibodies; 5.1 Introduction; 5.2 Structural and Functional Properties of Antibodies; 5.3 Polyclonal and Monoclonal Antibodies; 5.4 Antibody-Antigen Interactions; 5.5 Analytical Applications of Secondary Antibody-Antigen Interactions; 5.5.1 Agglutination Reactions 327 $a5.5.2 Precipitation Reactions 330 $aBioanalytical Chemistry provides a thorough introduction for students and practitioners with a broad range of backgrounds from chemistry to medicine. In so doing, it brings together many of the techniques commonly used by biochemists and molecular biologists. The text includes entire chapters on design and implementation of enzyme assays; mass spectrometry; and validation of new methods. Each chapter progresses from basic concepts to applications involving real samples, and ends with a set of problems, while an appendix contains selected answers. The authors have limited mathematical derivatio 606 $aAnalytical biochemistry 615 0$aAnalytical biochemistry. 676 $a572/.36 700 $aMikkelsen$b Susan R.$f1960-$01360558 701 $aCorto?n$b Eduardo$f1962-$01360559 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910780374003321 996 $aBioanalytical chemistry$93378058 997 $aUNINA LEADER 05224nam 2200805 a 450 001 9910961435403321 005 20251116180142.0 010 $a0-252-09371-2 010 $a1-283-99452-6 035 $a(CKB)2550000000996664 035 $a(EBL)3414198 035 $a(SSID)ssj0000821523 035 $a(PQKBManifestationID)12308592 035 $a(PQKBTitleCode)TC0000821523 035 $a(PQKBWorkID)10879663 035 $a(PQKB)10336435 035 $a(StDuBDS)EDZ0000340914 035 $a(OCoLC)1097104384$z(OCoLC)826684900$z(OCoLC)842264811$z(OCoLC)923496862$z(OCoLC)961602652$z(OCoLC)962609373$z(OCoLC)966210405$z(OCoLC)988431298$z(OCoLC)991964400$z(OCoLC)1037911180$z(OCoLC)1038649059$z(OCoLC)1045505501$z(OCoLC)1055317942$z(OCoLC)1066660542$z(OCoLC)1081272525$z(OCoLC)1153550754 035 $a(OCoLC)on1097104384 035 $a(MdBmJHUP)muse25145 035 $a(Au-PeEL)EBL3414198 035 $a(CaPaEBR)ebr10651017 035 $a(CaONFJC)MIL430702 035 $a(OCoLC)923496862 035 $a(Perlego)2554158 035 $a(MiAaPQ)EBC3414198 035 $a(EXLCZ)992550000000996664 100 $a20111115d2012 ub 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAfricans to Spanish America $eexpanding the diaspora /$fedited by Sherwin K. Bryant, Rachel Sarah O'Toole, Ben Vinson, III 205 $a1st ed. 210 $aUrbana $cUniversity of Illinois Press$d2012 215 $a1 online resource (290 p.) 225 1 $aNew Black studies series 300 $aDescription based upon print version of record. 311 08$a0-252-08001-7 311 08$a0-252-03663-8 320 $aIncludes bibliographical references (p. [229]-262) and index. 327 $aThe Shape of a Diaspora : The Movement of Afro-Iberians to Colonial Spanish America / Leo Garofalo -- African Diasporic Ethnicity in Mexico City to 1650 / Frank "Trey" Proctor -- To Be Free and Lucumi? : Ana de la Calle and Making African Diaspora Identities in Colonial Peru / Rachel Sarah O'Toole -- Between the Cross and the Sword : Religious Conquest and Maroon Legitimacy in Colonial Esmeraldas / Charles Beatty-Medina -- Finding Saints in an Alley : Afro-Mexicans in Early Eighteenth-Century Mexico City / Joan Cameron Bristol -- The Religious Servants of Lima, 1600-1700 / Nancy E. van Deusen -- Whitening Revisited : Nineteenth-Century Cuban Counterpoints / Karen Y. Morrison -- Tensions of Race, Gender, and Midwifery in Colonial Cuba / Michele B. Reid --The African American Experience in Comparative Perspective : The Current Question of the Debate / Herbert S. Klein. 330 $a"Exploring the connections between colonial Latin American historiography and the scholarship on the African Diaspora in the Spanish empires, Africans to Spanish America points to the continuities as well as disjunctures between the two fields of study. While a majority of the research on the colonial diaspora focuses on the Caribbean and Brazil, analysis of the regions of Mexico and the Andes open up new questions of community formation that incorporated Spanish legal strategies in secular and ecclesiastical institutions as well as articulations of multiple African identities. Therefore, it is critically important to expand the lens of the Diaspora framework that has come to shape so much of the recent scholarship on Africans in the Americas. Comprised of nine original essays, this volume is organized into three sections. Starting with voluntary and forced migrations across the Atlantic, Part I explores four distinct cases of identity construction that intersect with ongoing debates in African Diaspora scholarship regarding the models of continuity and creolization in the Americas. Part II interrogates how enslaved and free people employed their rights as Catholics to present themselves as civilized subjects, loyal Christians, and resisters to slavery. 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