LEADER 04062nam 2200661Ia 450 001 9910779848203321 005 20220929185133.0 010 $a1-280-20633-0 010 $a9786610206339 010 $a0-306-47058-6 024 7 $a10.1007/b112907 035 $a(CKB)111056485435356 035 $a(EBL)3035545 035 $a(SSID)ssj0000260175 035 $a(PQKBManifestationID)11193875 035 $a(PQKBTitleCode)TC0000260175 035 $a(PQKBWorkID)10191473 035 $a(PQKB)11274117 035 $a(DE-He213)978-0-306-47058-5 035 $a(MiAaPQ)EBC3035545 035 $a(Au-PeEL)EBL3035545 035 $a(CaPaEBR)ebr10048293 035 $a(CaONFJC)MIL20633 035 $a(OCoLC)923695963 035 $a(PPN)237931656 035 $a(EXLCZ)99111056485435356 100 $a20070330d2002 my 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aTopics in fluorescence spectroscopy$eprinciples$b[electronic resource] /$fedited by Joseph R. Lakowicz 205 $a1st ed. 2002. 210 $aNew York $cKluwer Academic$dc2002 215 $a1 online resource (449 p.) 225 1 $aTopics in fluorescence spectroscopy ;$vv.2 300 $aDescription based upon print version of record. 311 $a0-306-43875-5 320 $aIncludes bibliographical references and index. 327 $aFluorescence Anisotropy: Theory and Applications -- Fluorescence Quenching: Theory and Applications -- Resonance Energy Transfer -- Least-Squares Analysis of Fluorescence Data -- The Global Analysis of Fluorescence Intensity and Anisotropy Decay Data: Second-Generation Theory and Programs -- Fluorescence Polarization from Oriented Systems -- Fluorescence-Based Fiber-Optic Sensors -- Inhomogeneous Broadening of Electronic Spectra of Dye Molecules in Solutions. 330 $aFluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial two volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. The third volume will appear shortly. The first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3). 410 0$aTopics in fluorescence spectroscopy ;$vv.2. 606 $aDiagnostic imaging 606 $aFluorescence spectroscopy 606 $aLasers$xDiagnostic use 615 0$aDiagnostic imaging. 615 0$aFluorescence spectroscopy. 615 0$aLasers$xDiagnostic use. 676 $a543/.0858 701 $aLakowicz$b Joseph R$021246 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910779848203321 996 $aTopics in fluorescence spectroscopy$93678957 997 $aUNINA