LEADER 05582nam 2200661 a 450 001 9910778959603321 005 20230725054410.0 010 $a1-283-43385-0 010 $a9786613433855 010 $a981-4354-86-4 035 $a(CKB)2550000000079258 035 $a(EBL)840651 035 $a(SSID)ssj0000573494 035 $a(PQKBManifestationID)12207160 035 $a(PQKBTitleCode)TC0000573494 035 $a(PQKBWorkID)10543458 035 $a(PQKB)11427884 035 $a(MiAaPQ)EBC840651 035 $a(WSP)00008166 035 $a(Au-PeEL)EBL840651 035 $a(CaPaEBR)ebr10524650 035 $a(CaONFJC)MIL343385 035 $a(OCoLC)775586481 035 $a(EXLCZ)992550000000079258 100 $a20110928d2011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aScience and technology against microbial pathogens$b[electronic resource] $eresearch, development and evaluation : proceedings of the International Conference on Antimicrobial Research (ICAR2010), Valladolid, Spain, 3-5 November 2010 /$feditor, A. Mendez-Vilas 210 $aSingapore $cWorld Scientific$dc2011 215 $a1 online resource (447 p.) 300 $aDescription based upon print version of record. 311 $a981-4354-85-6 320 $aIncludes bibliographical references. 327 $aIntroduction; Contents; Antimicrobial Peptides; A new class of Scots pine antimicrobial proteins, which act by binding b-glucan Sanjeewani Sooriyaarachchi, Adrian Sua?rez Covarrubias, Wimal Ubhayasekera, Frederick O. Asiegbu and Sherry L. Mowbray; 1. Introduction; 2. Material and methods; 2.1 Cloning, expression and purification of Sp-AMP3; 2.2 Binding studies; 2.3 Antifungal activity; 2.4 Scanning electron microscopic studies; 2.5 Homology modeling; 3. Results and discussion; 4. Conclusions; References 327 $aAntimicrobial aza- -peptides: Structure-activity relationship? B. Legrand, M. Laurencin, C. Zatylny-Gaudin, J. Henry, A. Bondon and M. Baudy Floc'h1. Introduction; 2. Results and Discussion; 3. Conclusion; References; Differential antimicrobial activities of Human Beta-Defensins against Methicillin Resistant (MRSA) and Methicillin sensitive (MSSA) Staphylococcus aureus N. D. S. Herathge, J. T. George and D. A. Rowley; Introduction; Materials and Methods; Results; Discussion; References 327 $aIsolation of antimicrobial peptides from New Zealand spinach Tetragonia tetragonioides T. Neubauerova?, M. Mackova?, T. Macek, M. S?anda, M. Kra?lova?, I. Dolez?i?lkova? and P. N. Holmsgaard1. General remarks; 2. Materials and methods; 2.1 Extraction of peptides and proteins from spinach leaves; 2.2 Purification of crude extract; 2.3 Reverse-phase chromatography purification (RP-HPLC); 2.4 Microorganisms and antimicrobial assay; 2.5 Antimicrobial activity; 3. Results; 3.1 Isolation of antimicrobial peptides from spinach; 3.2 Antimicrobial assay; 3.3 Characterization by MS; 4. Discussion; References 327 $aLysostaphin: molecular changes that preserve staphylolytic activity S. Becker, J. Foster-Frey, A. Powell, H. Mohammadi, D. E. Kerr and D. M. Donovan1. Introduction; 2. Results and Discussion; 2.1 Effect of N- vs. C-terminal 6 x His tag on mLyso lytic activity; 2.2 Activity changes resulting from N125Q and N232Q mLyso mutations; 2.3 N-terminal deletions of mLyso between residue 1 and 31; Conclusions; References; Purification and characterization of antimicrobial peptides from fleshfly larvae haemolymph T. Neubauerova?, M. Mackova?, T. Macek, M. S?anda and Z. Voburka; 1. General remarks 327 $a2. Materials and methods2.1 Isolation of larval haemolymph; 2.2 Purification of antimicrobial peptides; 2.3 Ion-exchange and reversed-phase liquid chromatography (Ionex-FPLC, RP-HPLC); 2.4 Microorganisms and antimicrobial assay; 2.5 Tricine gel electrophoresis and electroblotting; 2.6 Mass spectrometry analysis; 3. Results and discussion; References; Structural and functional diversity of natural antimicrobial oligopeptides Aexander A. Zamyatnin; 1. Introduction; 2. Structure; 3. Functions; 4. Potential applications; References 327 $aThe role of Gram-negative envelope LPS on the bactericidal properties of proteins and peptides: the case of eosinophil cationic protein D. Pulido, M. Torrent, M. V. Nogue?s and E. Boix 330 $aThe aim of this book is to disseminate the most recent research in science and technology against microbial pathogens presented at the first edition of the ICAR Conference Series (ICAR2010) held in Valladolid, Spain, in November 2010. This volume is a compilation of 86 chapters written by active researchers that offer information and experiences and afford critical insights into anti-microbe strategies in a general context marked by the threat posed by the increasing antimicrobial resistance of pathogenic microorganisms. "Anti" is here taken in a wide sense as "against cell cycle, adhesion, or 606 $aDrug resistance in microorganisms$vCongresses 606 $aPathogenic bacteria$vCongresses 615 0$aDrug resistance in microorganisms 615 0$aPathogenic bacteria 676 $a579.3165 676 $a616.9/041 701 $aMe?ndez-Vilas$b A$01489100 712 12$aInternational Conference on Antimicrobial Research 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910778959603321 996 $aScience and technology against microbial pathogens$93709640 997 $aUNINA