LEADER 05415nam 2200661Ia 450 001 9910458669403321 005 20200520144314.0 010 $a1-280-62859-6 010 $a9786610628599 010 $a0-08-045476-3 035 $a(CKB)1000000000364371 035 $a(EBL)269534 035 $a(OCoLC)469385966 035 $a(SSID)ssj0000238806 035 $a(PQKBManifestationID)11218761 035 $a(PQKBTitleCode)TC0000238806 035 $a(PQKBWorkID)10234836 035 $a(PQKB)11118488 035 $a(MiAaPQ)EBC269534 035 $a(PPN)182572285 035 $a(Au-PeEL)EBL269534 035 $a(CaPaEBR)ebr10138208 035 $a(CaONFJC)MIL62859 035 $a(EXLCZ)991000000000364371 100 $a20050107d2005 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aRNA methodologies$b[electronic resource] $ea laboratory guide for isolation and characterization /$fRobert E. Farrell, Jr 205 $a3rd ed. 210 $aAmsterdam ;$aBoston $cElsevier/Academic Press$dc2005 215 $a1 online resource (794 p.) 300 $aDescription based upon print version of record. 311 $a0-12-249696-5 320 $aIncludes bibliographical references and index. 327 $aFront cover; Title page; Copyright page; Dedication; Table of contents; Preface; 1 RNA and the Cellular Biochemistry Revisited; Why Study RNA?; What Is RNA?; Assembly of Polynucleotides; Types of RNA; Stringency: Conditions That Influence Nucleic Acid Structure; Types of Double-Stranded Molecules; References and Suggested Reading; 2 Transcription and the Organization of Eukaryotic Genes; Transcription and the Central Dogma; Gene Organization; RNA Polymerases and the Products of Transcription; References; 3 Messenger RNA; Topology of a Typical mRNA Molecule; Stability in the Cytoplasm 327 $aLevels of RegulationReferences; 4 Resilient Ribonucleases; Rationale; Elimination of Ribonuclease Activity; Types of Ribonuclease Inhibitors; Preparation of Equipment and Reagents; Other Reagents Used to Control Nuclease Activity; Protocol: Synthesis of Vanadyl Ribonucleoside Complexes; References; 5 RNA Isolation Strategies; Rationale; Goals in the Purification of RNA; Lysis Buffer Formulations; Isolation of RNA with Guanidinium Buffers; Guanidinium-Acid-Phenol Extraction Techniques; Density Gradient Centrifugation; Simultaneous Isolation of RNA and DNA; The Word on Kits; Other Methods 327 $aShort- and Long-Term Storage of Purified RNAReferences; 6 The Truth About Tissues; Rationale; Tissue Culture or Tissue?; Homogenization Methods; RNA Isolation Strategies for Various Organs and Tissues; Protocol: LiCl-Urea Method for RNA Isolation from Tissue; Protocol: RNA Isolation from Lipid-Enriched Tissue; Purification of Polysome-Engaged mRNA; Collecting Samples in the Field; RNA ""Clean-Up"" Methods; References; 7 Isolation of Polyadenylated RNA; Rationale; Polyadenylation; The Poly(A) Caveat; Selection of Polyadenylated Molecules; Magnetic Bead Technology for Poly(A)+ Purification 327 $aOligo(dT)-Cellulose Column ChromatographyRapid, Non-Column Poly(A)+ Purification; References; 8 Quality Control for RNA Preparations; Rationale; Quality Control Technique 1: Electrophoretic Profile of the RNA; Quality Control Technique 2: Ultraviolet Spectrophotometry and Absorption Ratios; Quality Control Technique 3: Sample Capacity to Support RT-PCR; Quality Control Technique 4: Northern Analysis; Quality Control Technique 5: Sample Capacity to Support In Vitro Translation; References; 9 Dot Blot Analysis; Rationale; Advantages and Disadvantages; Appropriate Positive and Negative Controls 327 $aLimitations of the DataReferences; 10 Electrophoresis of RNA; Rationale; Normalization of Nucleic Acids; RNA Denaturing Systems for Agarose Gel Electrophoresis; Molecular Weight Standards; Gel Staining Techniques; Safety Considerations in Electrophoresis; Maintenance of Electrophoresis Equipment; Running Agarose Gels for the First Time: A Few Tips; References; 11 Photodocumentation and Image Analysis; Rationale; Photodocumentation; Digital Image Analysis; Suggested Reading; 12 Northern Analysis; Rationale; Choice of Filter Membrane; Handling and Filter Preparation 327 $aNorthern Transfer Techniques 330 $aThis laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on 606 $aRNA$xAnalysis$vLaboratory manuals 606 $aNucleic acids$xAnalysis$vLaboratory manuals 608 $aElectronic books. 615 0$aRNA$xAnalysis 615 0$aNucleic acids$xAnalysis 676 $a572.8/8 700 $aFarrell$b Robert E$0150036 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910458669403321 996 $aRNA methodologies$9898256 997 $aUNINA LEADER 03252nam 22006851 450 001 9910777500903321 005 20200514202323.0 010 $a1-4725-4587-7 010 $a1-281-29199-4 010 $a9786611291990 010 $a1-84714-439-X 024 7 $a10.5040/9781472545879 035 $a(CKB)1000000000412115 035 $a(EBL)437048 035 $a(OCoLC)609838776 035 $a(SSID)ssj0000278449 035 $a(PQKBManifestationID)11255527 035 $a(PQKBTitleCode)TC0000278449 035 $a(PQKBWorkID)10246209 035 $a(PQKB)10371983 035 $a(MiAaPQ)EBC437048 035 $a(Au-PeEL)EBL437048 035 $a(CaPaEBR)ebr10224801 035 $a(CaONFJC)MIL129199 035 $a(OCoLC)614500206 035 $a(UtOrBLW)bpp09255620 035 $a(EXLCZ)991000000000412115 100 $a20140929d2002 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 04$aThe Cyborg experiments $ethe extensions of the body in the media age /$fedited by Joanna Zylinska 210 1$aLondon ;$aNew York :$cContinuum,$d2002. 215 $a1 online resource (252 p.) 225 1 $aTechnologies 300 $aDescription based upon print version of record. 311 $a0-8264-5902-1 311 $a0-8264-5903-X 320 $aIncludes bibliographical references and index. 327 $aContributors: -- John Appleby, Rachel Armstrong, Fred Botting, Julie Clarke, Gary Hall, Chris Hables Gray, Meredith Jones, Orlan, Mark Poster, Jay Prosser, E. A. Scheer, Zoe? Sofia, Stelarc, Scott Wilson, Joanna Zylinska 330 8 $aThe Cyborg Experiments analyzes the challenges posed to corporeality by techology. Taking as their starting point the work of the highly influential performance artists Orlan and Stelarc, the essays in this timely and important collection raise a number of questions in relation to new conceptions of embodiment, identity and otherness in the age of new technologies: Has the body become obsolete? Does transgender challenge traditional ideas of agency? Have we always been cyborgs?In addition to highlighting the playful character of digital aesthetics, the contributors investigate ethical issues concerning the ownership of our bodies and the experiments we perform on them. In this way the book explores how humanism, and ideas of "the human", have been placed under increasing scrutiny as a result of new developments in science, media and communications.Contributors:John Appleby, Rachel Armstrong, Fred Botting, Julie Clarke, Gary Hall, Chris Hables Gray, Meredith Jones, Orlan, Mark Poster, Jay Prosser, E. A. Scheer, Zod Sofia, Stelarc, Scott Wilson, Joanna Zylinska 410 0$aTechnologies (London, England) 606 $aCyborgs 606 $aHuman-machine systems 606 $aRobotics$xSocial aspects 606 $2Philosophy 615 0$aCyborgs. 615 0$aHuman-machine systems. 615 0$aRobotics$xSocial aspects. 676 $a303.48/3 702 $aZylinska$b Joanna$f1971- 801 0$bUtOrBLW 801 1$bUtOrBLW 801 2$bUkLoBP 906 $aBOOK 912 $a9910777500903321 996 $aThe Cyborg experiments$93765561 997 $aUNINA