LEADER 03741nam 22006495 450 001 9910743349803321 005 20240426145507.0 010 $a981-16-4549-3 010 $a981-16-4550-7 010 $a981-16-4550-7 024 7 $a10.1007/978-981-16-4550-1 035 $a(MiAaPQ)EBC6897047 035 $a(Au-PeEL)EBL6897047 035 $a(CKB)21325621300041 035 $a(DE-He213)978-981-16-4550-1 035 $a(PPN)260830542 035 $a(EXLCZ)9921325621300041 100 $a20220225d2022 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aOptical Spectroscopic and Microscopic Techniques $eAnalysis of Biological Molecules /$fedited by Harekrushna Sahoo 205 $a1st ed. 2022. 210 1$aSingapore :$cSpringer Nature Singapore :$cImprint: Springer,$d2022. 215 $a1 online resource (260 pages) 311 08$aPrint version: Sahoo, Harekrushna Optical Spectroscopic and Microscopic Techniques Singapore : Springer Singapore Pte. Limited,c2022 9789811645495 320 $aIncludes bibliographical references and index. 327 $a1 Absorption Spectroscopy: What can we learn on conformational changes of Biomolecules? -- 2 Circular Dichroism Spectroscopy: Principle and Application -- 3 Protein/Ligand Interaction using Steady-State Fluorescence Spectroscopy -- 4 Fluorescence Anisotropy: Probing Rotational Dynamics of Biomolecules -- 5 Fluorescence Lifetime: A Multifaceted Tool for Exploring Biological Systems -- 6 From Ensemble FRET to Single-molecule Imaging: Monitoring individual cellular machinery in action -- 7 Nanosecond Time- Resolved Fluorescence Assays -- 8 Fluorescence Correlation Spectroscopy: A highly sensitive tool for probing intracellular molecular dynamics and disease diagnosis -- 9 Principles and applications of Fluorescence microscopy -- 10 Analysis of biomolecular dynamics under Fourier-transform infrared spectroscopy -- 11 Raman Spectroscopy in Biology: Perspectives and Emerging Frontiers. 330 $aThis book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques. 606 $aBiology$xTechnique 606 $aProteins 606 $aMolecular spectroscopy 606 $aBiological Techniques 606 $aProteins 606 $aMolecular Spectroscopy 606 $aBiomolècules$2thub 606 $aEspectroscòpia$2thub 608 $aLlibres electrònics$2thub 615 0$aBiology$xTechnique. 615 0$aProteins. 615 0$aMolecular spectroscopy. 615 14$aBiological Techniques. 615 24$aProteins. 615 24$aMolecular Spectroscopy. 615 7$aBiomolècules 615 7$aEspectroscòpia 676 $a572.36 702 $aSahoo$b Harekrushna 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910743349803321 996 $aOptical spectroscopic and microscopic techniques$93559267 997 $aUNINA