LEADER 03291nam 2200541 a 450 001 9910741174403321 005 20200520144314.0 010 $a3-319-00336-4 024 7 $a10.1007/978-3-319-00336-8 035 $a(CKB)2670000000371125 035 $a(EBL)1316915 035 $a(SSID)ssj0000897267 035 $a(PQKBManifestationID)11488260 035 $a(PQKBTitleCode)TC0000897267 035 $a(PQKBWorkID)10902104 035 $a(PQKB)10009163 035 $a(DE-He213)978-3-319-00336-8 035 $a(MiAaPQ)EBC1316915 035 $a(PPN)170489590 035 $a(EXLCZ)992670000000371125 100 $a20130318d2013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aHow free cationic polymer chains promote gene transfection /$fYue Yanan 205 $a1st ed. 2013. 210 $aNew York $cSpringer$d2013 215 $a1 online resource (108 p.) 225 1 $aSpringer theses : recognizing outstanding Ph.D. research,$x2190-5053 300 $aDescription based upon print version of record. 311 $a3-319-00335-6 320 $aIncludes bibliographical references. 327 $aIntroduction and background -- Revisiting complexation between DNA and polyethylenimine: the effect of uncomplexed chains free in the solution mixture on gene transfection -- Revisiting complexation between DNA and polyethylenimine: the effect of length of free polycationic chains on gene transfection -- Quantitative comparison of endocytosis and intracellular trafficking of DNA/polymer complexes in the absence/presence of free polycationic chains. 330 $aIn this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted ?proton sponge? model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors. 410 0$aSpringer theses. 606 $aAddition polymerization 615 0$aAddition polymerization. 676 $a572.33 700 $aYanan$b Yue$01064485 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910741174403321 996 $aHow Free Cationic Polymer Chains Promote Gene Transfection$92538435 997 $aUNINA