LEADER 04897nam 22006375 450 001 9910741142503321 005 20200703081801.0 010 $a3-030-33055-9 024 7 $a10.1007/978-3-030-33055-2 035 $a(CKB)4100000009939765 035 $a(MiAaPQ)EBC5986783 035 $a(DE-He213)978-3-030-33055-2 035 $a(PPN)242824293 035 $a(EXLCZ)994100000009939765 100 $a20191127d2019 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 14$aThe Optical Clearing Method $eA New Tool for Clinical Practice and Biomedical Engineering /$fby Luís Manuel Couto Oliveira, Valery Victorovich Tuchin 205 $a1st ed. 2019. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2019. 215 $a1 online resource (183 pages) 225 1 $aSpringerBriefs in Physics,$x2191-5423 311 $a3-030-33054-0 320 $aIncludes bibliographical references and index. 327 $aChapter1. Tissue Optics -- Chapter2. Controlling the optical properties of biological materials -- Chapter3. Typical optical clearing agents -- Chapter4. Major optical clearing mechanisms -- Chapter5. Measurements during optical clearing -- Chapter6. Data that can be acquired from optical clearing studies -- Chapter7. Optical clearing and tissue imaging -- Chapter8. Other applications of OCAs -- Chapter9. Future perspectives of the optical clearing method. 330 $aThis book describes the Optical Immersion Clearing method and its application to acquire information with importance for clinical practice and various fields of biomedical engineering. The method has proved to be a reliable means of increasing tissue transparency, allowing the investigator or surgeon to reach deeper tissue layers for improved imaging and laser surgery. This result is obtained by partial replacement of tissue water with an active optical clearing agent (OCA) that has a higher refractive index and is a better match for the refractive index of other tissue components. Natural tissue scattering is thereby reduced. An exponential increase in research using this method has occurred in recent years, and new applications have emerged, both in clinical practice and in some areas of biomedical engineering. Recent research has revealed that treating ex vivo tissues with solutions containing active OCAs in different concentrations produces experimental data to characterize drug delivery or to discriminate between normal and pathological tissues. The obtained drug diffusion properties are of interest for the pharmaceutical and organ preservation industry. Similar data can be estimated with particular interest for food preservation. The free water content evaluation is also of great interest since it facilitates the characterization of tissues to discriminate pathologies. An interesting new application that is presented in the book regards the creation of two optical windows in the ultraviolet spectral range through the application of the immersion method. These induced transparency windows open the possibility to diagnose and treat pathologies with ultraviolet light. This book presents photographs from the tissues we have studied and figures that represent the experimental setups used. Graphs and tables are also included to show the numerical results obtained in the sequential calculations performed. 410 0$aSpringerBriefs in Physics,$x2191-5423 606 $aBiophysics 606 $aBiological physics 606 $aBiomedical engineering 606 $aMicroscopy 606 $aBiological and Medical Physics, Biophysics$3https://scigraph.springernature.com/ontologies/product-market-codes/P27008 606 $aBiomedical Engineering and Bioengineering$3https://scigraph.springernature.com/ontologies/product-market-codes/T2700X 606 $aBiological Microscopy$3https://scigraph.springernature.com/ontologies/product-market-codes/L26000 606 $aBiomedical Engineering/Biotechnology$3https://scigraph.springernature.com/ontologies/product-market-codes/B24000 615 0$aBiophysics. 615 0$aBiological physics. 615 0$aBiomedical engineering. 615 0$aMicroscopy. 615 14$aBiological and Medical Physics, Biophysics. 615 24$aBiomedical Engineering and Bioengineering. 615 24$aBiological Microscopy. 615 24$aBiomedical Engineering/Biotechnology. 676 $a612.84 700 $aOliveira$b Luís Manuel Couto$4aut$4http://id.loc.gov/vocabulary/relators/aut$0838591 702 $aTuchin$b Valery Victorovich$4aut$4http://id.loc.gov/vocabulary/relators/aut 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910741142503321 996 $aThe Optical Clearing Method$93553622 997 $aUNINA