LEADER 01661nam 2200421 450 001 9910703643203321 005 20150401090209.0 035 $a(CKB)5470000002433280 035 $a(OCoLC)905969257 035 $a(EXLCZ)995470000002433280 100 $a20150401d2014 ua 0 101 0 $aeng 135 $aurmn||||||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 10$aMissile defense, DOD's report provides limited insight on improvements to homeland missile defense and acquisitions plans /$fCristina Chaplain 210 1$aWashington, DC :$cU.S. Government Accountability Office,$d2014. 215 $a1 online resource (7 pages) 300 $aTitle from title screen (viewed Mar. 26, 2015). 300 $a"July 17, 2014." 300 $a"GAO-14-626R." 320 $aIncludes bibliographical references. 517 3 $aMissile defense, Department of Defense's report provides limited insight on improvements to homeland missile defense and acquisitions plans 517 1 $aHomeland missile defense 606 $aBallistic missile defenses$zUnited States$xPlanning 606 $aNational security$zUnited States 607 $aUnited States$xDefenses 615 0$aBallistic missile defenses$xPlanning. 615 0$aNational security 700 $aChaplain$b Cristina T.$01381995 712 02$aUnited States.$bGovernment Accountability Office, 801 0$bGPO 801 1$bGPO 906 $aBOOK 912 $a9910703643203321 996 $aMissile defense, DOD's report provides limited insight on improvements to homeland missile defense and acquisitions plans$93493287 997 $aUNINA LEADER 05488nam 22006494a 450 001 9910829878103321 005 20230617040820.0 010 $a1-282-30744-4 010 $a9786612307447 010 $a0-470-31712-4 010 $a0-470-31796-5 035 $a(CKB)1000000000687577 035 $a(EBL)468845 035 $a(OCoLC)264621192 035 $a(SSID)ssj0000337507 035 $a(PQKBManifestationID)11297314 035 $a(PQKBTitleCode)TC0000337507 035 $a(PQKBWorkID)10294934 035 $a(PQKB)10546657 035 $a(MiAaPQ)EBC468845 035 $a(EXLCZ)991000000000687577 100 $a20030404d2004 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aExploration and analysis of DNA microarray and protein array data$b[electronic resource] /$fDhammika Amaratunga, Javier Cabrera 210 $aHoboken, NJ $cJohn Wiley$dc2004 215 $a1 online resource (270 p.) 225 1 $aWiley series in probability and statistics 300 $aDescription based upon print version of record. 311 $a0-471-27398-8 320 $aIncludes bibliographical references (p. 222-236) and indexes. 327 $aExploration and Analysis of DNA Microarray and Protein A rray Data; Contents; Preface; 1. A Brief Introduction; 1.1. A Note on Exploratory Data Analysis; 1.2. Computing Considerations and Software; 1.3. A Brief Outline of the Book; 2. Genomics Basics; 2.1. Genes; 2.2. DNA; 2.3. Gene Expression; 2.4. Hybridization Assays and Other Laboratory Techniques; 2.5. The Human Genome; 2.6. Genome Variations and Their Consequences; 2.7. Genomics; 2.8. The Role of Genomics in Pharmaceutical Research; 2.9. Proteins; 2.10. Bioinformatics; Supplementary Reading; Exercises; 3. Microarrays 327 $a3.1. Types of Microarray Experiments3.1.1. Experiment Type 1: Tissue-Specific Gene Expression; 3.1.2. Experiment Type 2: Developmental Genetics; 3.1.3. Experiment Type 3: Genetic Diseases; 3.1.4. Experiment Type 4: Complex Diseases; 3.1.5. Experiment Type 5: Pharmacological Agents; 3.1.6. Experiment Type 6: Plant Breeding; 3.1.7. Experiment Type 7: Environmental Monitoring; 3.2. A Very Simple Hypothetical Microarray Experiment; 3.3. A Typical Microarray Experiment; 3.3.1. Microarray Preparation; 3.3.2. Sample Preparation; 3.3.3. The Hybridization Step; 3.3.4. Scanning the Microarray 327 $a3.3.5. Interpreting the Scanned Image3.4. Multichannel cDNA Microarrays; 3.5. Oligonucleotide Arrays; 3.6. Bead-Based Arrays; 3.7. Confirmation of Microarray Results; Supplementary Reading and Electronic References; Exercises; 4. Processing the Scanned Image; 4.1. Converting the Scanned Image to the Spotted Image; 4.1.1. Gridding; 4.1.2. Segmentation; 4.1.3. Quantification; 4.2. Quality Assessment; 4.2.1. Visualizing the Spotted Image; 4.2.2. Numerical Evaluation of Array Quality; 4.2.3. Spatial Problems; 4.2.4. Spatial Randomness; 4.2.5. Quality Control of Arrays 327 $a4.2.6. Assessment of Spot Quality4.3. Adjusting for Background; 4.3.1. Estimating the Background; 4.3.2. Adjusting for the Estimated Background; 4.4. Expression Level Calculation for Two-Channel cDNA Microarrays; 4.5. Expression Level Calculation for Oligonucleotide Arrays; 4.5.1. The Average Difference; 4.5.2. A Weighted Average Difference; 4.5.3. Perfect Matches Only; 4.5.4. Background Adjustment Approach; 4.5.5. Model-Based Approach; 4.5.6. Absent-Present Calls; Supplementary Reading; Exercises; 5. Preprocessing Microarray Data; 5.1. Logarithmic Transformation 327 $a5.2. Variance Stabilizing Transformations5.3. Sources of Bias; 5.4. Normalization; 5.5. Intensity-Dependent Normalization; 5.5.1. Smooth Function Normalization; 5.5.2. Quantile Normalization; 5.5.3. Normalization of Oligonucleotide Arrays; 5.5.4. Normalization of Two-Channel Arrays; 5.5.5. Spatial Normalization; 5.5.6. Stagewise Normalization; 5.6. Judging the Success of a Normalization; 5.7. Outlier Identification; 5.7.1 Nonresistant Rules for Outlier Identification; 5.7.2. Resistant Rules for Outlier Identification; 5.8. Assessing Replicate Array Quality; Exercises; 6. Summarization 327 $a6.1. Replication 330 $aA cutting-edge guide to the analysis of DNA microarray data Genomics is one of the major scientific revolutions of this century, and the use of microarrays to rapidly analyze numerous DNA samples has enabled scientists to make sense of mountains of genomic data through statistical analysis. Today, microarrays are being used in biomedical research to study such vital areas as a drug's therapeutic value-or toxicity-and cancer-spreading patterns of gene activity. Exploration and Analysis of DNA Microarray and Protein Array Data answers the need for a comprehensive, cutting-edge overview of th 410 0$aWiley series in probability and statistics. 606 $aDNA microarrays$xStatistical methods 606 $aProtein microarrays$xStatistical methods 615 0$aDNA microarrays$xStatistical methods. 615 0$aProtein microarrays$xStatistical methods. 676 $a572.8 676 $a572.8636 700 $aAmaratunga$b Dhammika$f1956-$01667367 701 $aCabrera$b Javier$0614089 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910829878103321 996 $aExploration and analysis of DNA microarray and protein array data$94106558 997 $aUNINA