LEADER 01684nam 2200349 450 001 9910678590103321 005 20230502082201.0 010 $a1-83768-027-2 035 $a(CKB)5840000000237825 035 $a(NjHacI)995840000000237825 035 $a(EXLCZ)995840000000237825 100 $a20230502d2023 uy 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aCryopreservation $eApplications and Challenges /$fMarian Quain, editor 210 1$aLondon :$cIntechOpen,$d2023. 215 $a1 online resource (168 pages) 311 $a1-83768-026-4 330 $aCryopreservation is the preservation of cells at sub-zero temperatures. Although useful, the process is not without its challenges. One of the major challenges is the formation of ice crystals in the cells to be preserved. However, vitrification, where glass is formed instead of crystals, can be achieved using cryoprotection agents (CPAs). This book provides a comprehensive overview of cryopreservation and its applications. It discusses advancements in the field, challenges, guidelines, and recommendations for successful germplasm conservation. Chapters discuss the use of CPAs, cryopreservation of fish sperm, cryopreservation of oocytes and sperms, female fertility preservation, cryopreservation of large structures and tissues, and much more. 606 $aCryobiology 615 0$aCryobiology. 676 $a570.752 702 $aQuain$b Marian 801 0$bNjHacI 801 1$bNjHacl 906 $aBOOK 912 $a9910678590103321 996 $aCryopreservation$92951916 997 $aUNINA LEADER 01759oam 2200481 a 450 001 9910698657103321 005 20091029070234.0 035 $a(CKB)3460000000106397 035 $a(OCoLC)312162804 035 $a(EXLCZ)993460000000106397 100 $a20090304d2009 ua 0 101 0 $aeng 135 $aurbn||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aComprehensive immigration reform$b[electronic resource] $ehearing before the Committee on the Judiciary, United States Senate, One Hundred Ninth Congress, first session, July 26, 2005 210 1$aWashington :$cU.S. G.P.O.,$d2009. 215 $av, 243 pages $cdigital, PDF file 225 1 $aS. hrg. ;$v109-1031 300 $aTitle from title screen (viewed on Mar. 4, 2009). 300 $a"Serial no. J-109-34." 320 $aIncludes bibliographical references. 517 $aComprehensive immigration reform 606 $aNoncitizens$xGovernment policy$zUnited States 606 $aIllegal immigration$xGovernment policy$zUnited States 606 $aForeign workers$xGovernment policy$zUnited States 606 $aEmigration and immigration law$zUnited States 606 $aIllegal immigration 607 $aUnited States$xEmigration and immigration$xGovernment policy 615 0$aNoncitizens$xGovernment policy 615 0$aIllegal immigration$xGovernment policy 615 0$aForeign workers$xGovernment policy 615 0$aEmigration and immigration law 615 0$aIllegal immigration. 801 0$bGPO 801 1$bGPO 801 2$bOCLCA 801 2$bGPO 906 $aBOOK 912 $a9910698657103321 996 $aComprehensive immigration reform$93091449 997 $aUNINA