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001 9910695368103321
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035   $a(CKB)5470000002369068
035   $a(OCoLC)71782070
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100   $a20060927d2006      ua             0
101 0 $aeng
135   $aurbn|---|||||
181   $ctxt$2rdacontent
182   $cc$2rdamedia
183   $acr$2rdacarrier
200 14$aThe LC/MS quantification of vardenafil (LevitraŽ) in postmortem biological specimens$b[electronic resource] $efinal report /$fRobert D. Johnson, Russell J. Lewis, Mike K. Angier
210  1$aWashington, D.C. :$cFederal Aviation Administration, Office of Aerospace Medicine ;$aFt. Belvior, VA :$cAvailable to the public through the Defense Technical Information Center ;$aSpringfield, Va. :$cAvailable to the public through the National Technical Information Service,$d2006.
215   $ai, 10 pages $cdigital, PDF file
300   $aTile from title screen (viewed on Sept. 27, 2006).
300   $a"July 2006"
300   $a"DOT/FAA/AM-06/17."
320   $aIncludes bibliographical references (page 10).
330   $aDuring the investigation of aviation accidents, postmortem specimens from accident victims are submitted to the Federal Aviation Administration's Civil Aerospace Medical Institute (CAMI) for toxicological analysis. As new medications are introduced to the market and are subsequently used by aviation accident victims, CAMI's forensic toxicology laboratory is tasked with developing analytical methods for the determination of these compounds. This report presents a rapid and reliable method for the identification and quantitation of vardenafil (LevitraŽ) in biological specimens. This procedure utilizes sildenafil-d8, which structurally is closely related to vardenafil, as an internal standard for more accurate and reliable quantitation. The method incorporates solid phase extraction and LC/MS/MS and MS/MS/MS utilizing an atmospheric pressure chemical ionization ion trap mass spectrometer in the positive chemical ionization mode. Solid-phase extraction proved to be exceptionally efficient providing recoveries that ranged from 94-97%. The limit of detection for vardenafil was determined to be 0.19 ng/mL. The linear dynamic range for this compound was 0.39-200 ng/mL. This method was successfully applied to postmortem fluid and tissue specimens obtained from an aviation accident victim. This novel analytical procedure proved to be simple, accurate, and robust for the identification and quantitation of vardenafil in postmortem specimens.
517   $aLC/MS quantification of vardenafil 
606   $aForensic toxicology$zUnited States
606   $aImpotence
606   $aAircraft accidents$xInvestigation$zUnited States
615  0$aForensic toxicology
615  0$aImpotence.
615  0$aAircraft accidents$xInvestigation
700   $aJohnson$b Robert D$g(Robert David),$f1974-$01382527
701   $aLewis$b Russell J$01382528
701   $aAngier$b Mike K$01384636
712 02$aUnited States.$bOffice of Aerospace Medicine.
712 02$aUnited States.$bFederal Aviation Administration.
712 02$aCivil Aerospace Medical Institute.
801  0$bGPO
801  1$bGPO
906   $aBOOK
912   $a9910695368103321
996   $aThe LC$93431207
997   $aUNINA