LEADER 03608nam 22004935 450 001 9910693978403321 005 20240322090622.0 010 $a9786610820726 010 $a9781280820724 010 $a1280820721 010 $a9781592590827 010 $a1592590829 024 7 $a10.1385/1592590829 035 $a(CKB)1000000000239855 035 $a(VLeBooks)9781592590827 035 $a(MiAaPQ)EBC3036763 035 $a(DE-He213)978-1-59259-082-7 035 $a(EXLCZ)991000000000239855 100 $a20100301d2000 u| 0 101 0 $aeng 135 $aurnn|008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aCeliac Disease $eMethods and Protocols /$fedited by Michael N. Marsh 205 $a1st ed. 2000. 210 1$aTotowa, NJ :$cHumana Press :$cImprint: Humana,$d2000. 215 $a1 online resource (288 p.) 225 1 $aMethods in Molecular Medicine,$x1940-6037 ;$v41 311 08$a9780896036505 311 08$a0896036502 327 $aCeliac Disease -- Genotyping Methodologies -- From Linkage to Genes -- Linkage and the Transmission Disequilibrium Test in Complex Traits -- Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats -- Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry -- Synthetic Peptide Libraries for T-Cell Epitope Identification -- Characterization of HLA-DQ-Specific Peptide-Binding Motifs -- Studies of Gliadin-Specific T-Cells in Celiac Disease -- Morphometric Analysis of Intestinal Mucosa -- Morphometry of Rectal Mucosa -- Organ Culture of Rectal Mucosa -- In Situ Hybridization -- Measurements of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease -- Immunohistochemistry in Research and Diagnosis of Celiac Disease -- Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease -- Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease -- Whole Gut Lavage Fluid Analysis. 330 $aWith the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today. 410 0$aMethods in Molecular Medicine,$x1940-6037 ;$v41 606 $aMicrobiology 606 $aMicrobiology 615 0$aMicrobiology. 615 14$aMicrobiology. 676 $a616.34 702 $aMarsh$b Michael N$4edt$4http://id.loc.gov/vocabulary/relators/edt 906 $aBOOK 912 $a9910693978403321 996 $aCeliac disease$9879765 997 $aUNINA