LEADER 03780nam 2200601 450 001 9910688234603321 005 20230621141035.0 035 $a(CKB)3710000000586895 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/40500 035 $a(EXLCZ)993710000000586895 100 $a20160208d2015uuuu fy| 0 101 0 $aeng 135 $aurcu#---uuuuu 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aAgrobacterium biology and its application to transgenic plant production$b[electronic resource] /$fedited by: Hau-Hsuan Hwang, Erh-Min Lai and Stanton B. Gelvin 210 $cFrontiers Media SA$d2015 210 31$a[Lausanne, Switzerland] :$cFrontiers Media SA,$d2015. 215 $a1 online resource (165 pages) $cillustrations; digital, PDF file(s) 225 0 $aFrontiers Research Topics 225 1 $aFrontiers in Plant Science 225 1 $aFrontiers in Microbiology 311 $a2-88919-574-0 320 $aIncludes bibliographical references. 330 3 $aThe broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. A recently discovered bacterial type VI secretion system may also export bacterial proteins and impact transformation. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. Agrobacterium-mediated genetic transformation is easy to use, relatively inexpensive, and generally results in a low copy number of transgene insertions when compared to other means of plant transformation, such as particle bombardment. In this research topic, we shall provide updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes. 410 0$aFrontiers in Plant Science. 410 0$aFrontiers in Microbiology. 606 $aAgrobacterium 606 $aPathogenic microorganisms 606 $aPlants$xMicrobiology 610 $aAgrobacterium 610 $acrown gall 610 $aQuorum Sensing 610 $aplant defense 610 $aT DNA 610 $aVirulence 610 $aBiofilm 610 $aAttachment 610 $agenetic transformation 610 $aMembrane lipid 615 0$aAgrobacterium. 615 0$aPathogenic microorganisms. 615 0$aPlants$xMicrobiology. 700 $aErh Min Lai$4auth$01352892 702 $aStanton B Gelvin$4auth 702 $aHau Hsuan Hwang$4auth 712 02$aFrontiers Research Foundation, 801 2$bUkMaJRU 906 $aBOOK 912 $a9910688234603321 996 $aAgrobacterium biology and its application to transgenic plant production$93205463 997 $aUNINA