LEADER 01684nam 2200349 450 001 9910678590103321 005 20230502082201.0 010 $a1-83768-027-2 035 $a(CKB)5840000000237825 035 $a(NjHacI)995840000000237825 035 $a(EXLCZ)995840000000237825 100 $a20230502d2023 uy 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aCryopreservation $eApplications and Challenges /$fMarian Quain, editor 210 1$aLondon :$cIntechOpen,$d2023. 215 $a1 online resource (168 pages) 311 $a1-83768-026-4 330 $aCryopreservation is the preservation of cells at sub-zero temperatures. Although useful, the process is not without its challenges. One of the major challenges is the formation of ice crystals in the cells to be preserved. However, vitrification, where glass is formed instead of crystals, can be achieved using cryoprotection agents (CPAs). This book provides a comprehensive overview of cryopreservation and its applications. It discusses advancements in the field, challenges, guidelines, and recommendations for successful germplasm conservation. Chapters discuss the use of CPAs, cryopreservation of fish sperm, cryopreservation of oocytes and sperms, female fertility preservation, cryopreservation of large structures and tissues, and much more. 606 $aCryobiology 615 0$aCryobiology. 676 $a570.752 702 $aQuain$b Marian 801 0$bNjHacI 801 1$bNjHacl 906 $aBOOK 912 $a9910678590103321 996 $aCryopreservation$92951916 997 $aUNINA