LEADER 05130nam 2200625 450 001 9910677693003321 005 20230421045147.0 010 $a1-281-84256-7 010 $a9786611842567 010 $a3-527-61461-3 010 $a3-527-61460-5 035 $a(CKB)1000000000557222 035 $a(EBL)481892 035 $a(OCoLC)742612554 035 $a(SSID)ssj0000108456 035 $a(PQKBManifestationID)11141638 035 $a(PQKBTitleCode)TC0000108456 035 $a(PQKBWorkID)10036162 035 $a(PQKB)11393281 035 $a(MiAaPQ)EBC481892 035 $a(EXLCZ)991000000000557222 100 $a20160820h19971997 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aBacterial toxins $etools in cell biology and pharmacology /$fKlaus Aktories (Ed.) 210 1$aLondon, [England] :$cChapman & Hall,$d1997. 210 4$dİ1997 215 $a1 online resource (336 p.) 225 1 $aLaboratory Companion 300 $a"With 42 Figures"--Title page. 311 $a3-527-30881-4 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aBacterial Toxins; Contents; CHAPTER 1 . Cholera Toxin: Mechanism of Action and Potential Use in Vaccine Development; 1.1 Introduction; 1.2 Molecular Aspects of Cholera Toxin Action; 1.2.1 Structure and Relationship to Other Toxins; 1.2.2 Toxin Entry into Cells and Events Leading to Pathogenesis; 1.2.3 Enzymology of Cholera Toxin; 1.2.4 In Vitro Stimulation of Cholera Toxin Activity by ARF; 1.3 Practical Aspects of Cholera Toxin Use; 1.3.1 Vaccine and Vaccine Development; 1.3.2 Cholera Toxin as a Molecular Tool; 1.4 Summary 327 $aCHAPTER 2 . Cholera Toxin and Escherichia coli Heat-labile Enterotoxin: Biochemical Methods for Assessing Enzymatic Activities2.1 Introduction; 2.2 General Information on CT. LT. ARF and Reagents; 2.2.1 Sources, Purification, and Activation of CTA and LTA; 2.2.2 Sources and Purification of ARF; 2.2.3 Reagents and Materials; 2.2.4 Stock Solutions; 2.3 Assay 1 : The Gsa Assay; 2.3.1 Additional Reagents and Materials Required; 2.3.2 Protocol; 2.4 Assay 2: The Agmatine Assay; 2.4.1 Additional Reagents and Materials Required; 2.4.2 Protocol; 2.5 Assay 3: Auto-ADP-ribosylation Assay 327 $a2.5.1 Additional Reagents and Materials Required2.5.2 Protocol; 2.6 Assay 4: NAD Glycohydrolase Assay; 2.6.1 Additional Reagents and Materials Required; 2.6.2 Protocol; 2.7 Comments and Considerations; 2.7.1 Appropriate Controls and Analysis of Data; 2.7.1.1 Controls; 2.7.1.2 Data analysis; 2.7.2 Optimization Interfering Substances, Troubleshooting, and Assay; 2.7.2.1 Interfering substances; 2.7.2.2 Troubleshooting; 2.7.2.3 Assay optimization; 2.7.3 Consideration for the Use of ARF; 2.7.3.1 Lipid/Detergent and Nucleotide Requirements; 2.7.3.2 Development of other Assay Conditions 327 $aCHAPTER 3 . Pertussis Toxin3.1 Introduction; 3.2 Genetic Regulation of Pertussis Toxin Production; 3.3 Biogenesis of Pertussis Toxin; 3.4 Receptor-binding and Translocation; 3.5 ADP-ribosyltransferase Activity and Enzyme Mechanism; 3.6 Biological Activities and Role of Pertussis Toxin in Pathogenesis; CHAPTER 4 . Pertussis Toxin as a Cell Biology Tool; 4.1 Introduction; 4.2 Pertussis Toxin as a Tool to Modify Cellular Functions; 4.2.1 Cell Culture of Bordetella pertussis; 4.2.2 Source of Pertussis Toxin and Preparation of Solution 327 $a4.2.3 Treatment of Mammalian Cell Cultures with Pertussis Toxin4.3 Pertussis Toxin as a Tool to Study Cellular Components; 4.3.1 Activation of Pertussis Toxin for in in vitro ADP- ribosylation; 4.3.2 Preparation of Cell Homogenates and Fractions; 4.3.3 ADP-ribosylation of Membrane Proteins by Pertussis Toxin; 4.3.4 ADP-ribosylation of Proteins by Pertussis Toxin; 4.3.5 Preparation of Samples for SDS-PAGE; 4.3.6 Cleavage of ADP-ribose from Ga Subunits; 4.4 SDS-Gel Electrophoresis; 4.5 Reagents and Chemicals; CHAPTER 5 . Clostridium botulinum ADP-ribosyltransferase C3; 5.1 Introduction 327 $a5.2 The Family of C3-like Transferases 330 $aThis is a survey of well characterized and recently discovered bacterial protein toxins. Leading investigators of the respective toxins review the various molecular mechanisms of action, ranging from toxin-induced ADP-ribosylation up to membrane perforation by pore-forming toxins. Thy also describe the consequences on host physiology before focusing on potential applications as cell biological and pharmacological tools for research and medical applications. Detailed descriptions of the methodology include the engineering and use of modified and chimeric toxins for better performance.A soli 410 0$aLaboratory companion. 606 $aBacterial toxins$vLaboratory manuals 615 0$aBacterial toxins 676 $a579.3165 676 $a615.95293 702 $aAktories$b K. 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910677693003321 996 $aBacterial toxins$92087346 997 $aUNINA