LEADER 03845nam 22005055 450 001 9910676687303321 005 20251017110131.0 010 $a9781071627648 010 $a1071627643 024 7 $a10.1007/978-1-0716-2764-8 035 $a(CKB)5860000000298373 035 $a(NjHacI)995860000000298373 035 $a(PPN)268205760 035 $a(DE-He213)978-1-0716-2764-8 035 $a(DNLM)9918751770206676 035 $a(EXLCZ)995860000000298373 100 $a20230220d2023 u| 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aAll-Optical Methods to Study Neuronal Function /$fedited by Eirini Papagiakoumou 205 $a1st ed. 2023. 210 1$aNew York, NY :$cSpringer US :$cImprint: Humana,$d2023. 215 $a1 online resource (xiii, 418 pages) $cillustrations 225 1 $aNeuromethods,$x1940-6045 ;$v191 311 08$a9781071627631 311 08$a1071627635 320 $aIncludes bibliographical references and index. 327 $aOptical Manipulation and Recording of Neural Activity with Wavefront Engineering -- Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments -- Light-Based Neuronal Circuit Probing in Living Brains at High-Resolution: Constraints and Layouts for Integrating Neuronal Activity Recording and Modulation in Three Dimensions -- High Speed All-Optical Neural Interfaces with 3D Temporally Focused Holography -- An All-Optical Physiology Pipeline towards Highly Specific and Artefact-Free Circuit Mapping -- Spatial and Temporal Considerations of Optogenetic Tools in an All-Optical Single-Beam Experiment -- Miniature Multiphoton Microscopes for Recording Neural Activity in Freely Moving Animals -- Optogenetics and Light Sheet Microscopy -- Widefield Multiphoton Imaging at Depth with Temporal Focusing -- High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectioning Widefield Microscopy -- Optical and Analytical Methods to Visualize and Manipulate Contrical Ensembles and Behavior -- Illuminating Neural Computation using Precision Optogenetics-Controlled Synthetic Perception -- Spectrally-Focused Stimulated Raman Scattering (sf-SRS) Microscopy for Label-Free Investigations of Molecular Mechanisms in Living Organisms. 330 $aThis open access volume provides an overview of the latest methods used to study neuronal function with all-optical experimental approaches, where light is used for both stimulation and monitoring of neuronal activity. The chapters in this book cover topics over a broad range, from fundamental background information in both physiology and optics in the context of all-optical neurophysiology experiments, to the design principles and hardware implementation of optical methods used for photoactivation and imaging. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, All-Optical Methods to Study Neuronal Function is a valuable resource for researchers in various disciplines such as physics, engineering, and neuroscience. This book will serve as a guide to establish useful references for groups starting out in this field, and provide insight on the optical systems, actuators, and sensors. . 410 0$aNeuromethods,$x1940-6045 ;$v191 606 $aNeurosciences 606 $aNeuroscience 615 0$aNeurosciences. 615 14$aNeuroscience. 676 $a574.1 702 $aPapagiakoumou$b Eirini 801 0$bNjHacI 801 1$bNjHacl 906 $aBOOK 912 $a9910676687303321 996 $aAll-Optical Methods to Study Neuronal Function$93066764 997 $aUNINA