LEADER 05368nam 22006614a 450 001 9910676633003321 005 20230721025925.0 010 $a1-280-72166-9 010 $a9786610721665 010 $a0-470-08795-1 010 $a0-470-08794-3 035 $a(CKB)1000000000355408 035 $a(EBL)281847 035 $a(OCoLC)476027151 035 $a(SSID)ssj0000174659 035 $a(PQKBManifestationID)11171696 035 $a(PQKBTitleCode)TC0000174659 035 $a(PQKBWorkID)10174632 035 $a(PQKB)10516282 035 $a(MiAaPQ)EBC281847 035 $a(EXLCZ)991000000000355408 100 $a20060523d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aHPLC for pharmaceutical scientists$b[electronic resource] /$fedited by Yuri Kazakevich, Rosario LoBrutto 210 $aHoboken, N.J. $cWiley-Interscience$dc2007 215 $a1 online resource (1136 p.) 300 $aDescription based upon print version of record. 311 $a0-471-68162-8 320 $aIncludes bibliographical references and indexes. 327 $aHPLC FOR PHARMACEUTICAL SCIENTISTS; CONTENTS; PREFACE; CONTRIBUTORS; PART I HPLC THEORY AND PRACTICE; 1 Introduction; 1.1 Chromatography in the Pharmaceutical World; 1.2 Chromatographic Process; 1.3 Classification; 1.4 History of Discovery and Early Development (1903-1933); 1.5 General Separation Process; 1.5.1 Modern HPLC Column; 1.5.2 HPLC System; 1.6 Types of HPLC; 1.6.1 Normal-Phase Chromatography (NP HPLC); 1.6.2 Reversed-Phase HPLC (RP HPLC or RPLC); 1.6.3 Ion-Exchange Chromatography (IEX); 1.6.4 Size-Exclusion Chromatography (SEC); 1.7 HPLC Descriptors (Vr, k, N, etc.) 327 $a1.7.1 Retention Volume1.7.2 Void Volume; 1.7.3 Retention Factor; 1.7.4 Selectivity; 1.7.5 Efficiency; 1.7.6 Resolution; References; 2 HPLC Theory; 2.1 Introduction; 2.2 Basic Chromatographic Descriptors; 2.3 Efficiency; 2.4 Resolution; 2.5 HPLC Retention; 2.6 Retention Mechanism; 2.7 General Column Mass Balance; 2.8 Partitioning Model; 2.9 Adsorption Model; 2.10 Total and Excess Adsorption; 2.11 Mass Balance in Adsorption Model; 2.12 Adsorption of the Eluent Components; 2.13 Void Volume Considerations; 2.14 Thermodynamic Relationships; 2.14.1 Effect of the Eluent Composition 327 $a2.15 Adsorption-Partitioning Retention Mechanism2.16 Secondary Equilibria; 2.16.1 Inclusion of Secondary Equilibria in the Mass Balance; 2.16.2 Salt Effect; 2.17 Gradient Elution Principles; 2.18 Types of Analyte Interactions with the Stationary Phase; 2.19 Conclusion; References; 3 Stationary Phases; 3.1 Introduction; 3.2 Type of Packing Material (Porous, Nonporous, Monolithic); 3.3 Base Material (Silica, Zirconia, Alumina, Polymers); 3.4 Geometry; 3.4.1 Shape (Spherical/Irregular); 3.4.2 Particle Size Distribution; 3.4.3 Surface Area; 3.4.4 Pore Volume; 3.4.5 Surface Geometry 327 $a3.5 Adsorbent Surface Chemistry3.5.1 Surface Chemistry of the Base Material; 3.5.2 Silica; 3.5.3 Silica Hybrid; 3.5.4 Polymeric Packings; 3.5.5 Zirconia (Metal Oxides); 3.5.6 Porous Carbon (or Carbon-Coated Phases); 3.6 Surface of Chemically Modified Material; 3.6.1 Limits of Surface Modification; 3.6.2 Chemical Modification; 3.6.3 Types of Bonded Phases; 3.6.4 Structure of the Bonded Layer; 3.6.5 Density of Bonded Ligands; 3.6.6 Residual Silanoles; 3.6.7 Surface Area of Modified Adsorbent; 3.7 Polymer-Based Adsorbents; 3.8 Stationary Phases for Chiral Separations 327 $a3.8.1 Polysaccharide-Coated Phases3.8.2 Pirkle-Type Phases; 3.8.3 Protein Phases; 3.8.4 Molecular Imprinted Polymers for Chiral Separations; 3.9 Columns; 3.9.1 Capillary/Monolithic/Packed Columns; 3.9.2 Column Cleaning; 3.9.3 Column Void Volume; 3.9.4 Mass of Adsorbent in the Column; References; 4 Reversed-Phase HPLC; 4.1 Introduction; 4.2 Retention in Reversed-Phase HPLC; 4.3 Stationary Phases for RPLC; 4.4 Mobile Phases for RPLC; 4.4.1 Eluent Composition and Solvent Strength of the Mobile Phase; 4.4.2 Type of Organic Modifier 327 $a4.4.3 Selectivity as a Function of Type and Concentration of Organic Composition 330 $aHPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Pr 606 $aHigh performance liquid chromatography 606 $aDrugs$xAnalysis 606 $aClinical chemistry 615 0$aHigh performance liquid chromatography. 615 0$aDrugs$xAnalysis. 615 0$aClinical chemistry. 676 $a615.19 676 $a615.1901 676 $a615/.1901 701 $aKazakevich$b Yuri$01341027 701 $aLoBrutto$b Rosario$01341028 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910676633003321 996 $aHPLC for pharmaceutical scientists$93063321 997 $aUNINA LEADER 01693oam 2200529 450 001 9910708669103321 005 20180809141010.0 035 $a(CKB)5470000002470536 035 $a(OCoLC)1031029656 035 $a(EXLCZ)995470000002470536 100 $a20180411d2017 ua 0 101 0 $aeng 135 $aurcn||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aColorado's forest resources, 2004-2013 /$fMichael T. Thompson [and ten others] 210 1$aFort Collins, CO :$cU.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station,$d2017. 215 $a1 online resource (iv, 136 pages) $ccolor illustrations, color maps 225 1 $aResource bulletin ;$vRMRS-RB-23 300 $a"March 2017." 320 $aIncludes bibliographical references (pages 73-80). 606 $aForests and forestry$zColorado$vStatistics 606 $aForest surveys$zColorado 606 $aForest surveys$2fast 606 $aForests and forestry$2fast 607 $aColorado$2fast 608 $aStatistics.$2fast 608 $aOnline resources. 608 $aStatistics.$2lcgft 615 0$aForests and forestry 615 0$aForest surveys 615 7$aForest surveys. 615 7$aForests and forestry. 700 $aThompson$b Michael T.$01387495 712 02$aRocky Mountain Research Station (Fort Collins, Colo.), 801 0$bGPO 801 1$bGPO 801 2$bGPO 801 2$bMERUC 801 2$bAGL 801 2$bOCLCA 801 2$bGPO 906 $aBOOK 912 $a9910708669103321 996 $aColorado's forest resources, 2004-2013$93477127 997 $aUNINA