LEADER 03662nam 22006255 450 001 9910631093503321 005 20251009102811.0 010 $a9783031108365$b(electronic bk.) 010 $z9783031108358 024 7 $a10.1007/978-3-031-10836-5 035 $a(MiAaPQ)EBC7141577 035 $a(Au-PeEL)EBL7141577 035 $a(CKB)25360918100041 035 $a(OCoLC)1351200448 035 $a(PPN)266355021 035 $a(DE-He213)978-3-031-10836-5 035 $a(EXLCZ)9925360918100041 100 $a20221116d2022 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aFlow Cytometry Today $eEverything You Need to Know about Flow Cytometry /$fby Claudio Ortolani 205 $a1st ed. 2022. 210 1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2022. 215 $a1 online resource (556 pages) 225 1 $aBiomedical and Life Sciences Series 311 08$aPrint version: Ortolani, Claudio Flow Cytometry Today Cham : Springer International Publishing AG,c2022 9783031108358 320 $aIncludes bibliographical references and index. 327 $aChapter 1. General Principles -- Chapter 2. Signals ? Scattering -- Chapter 3. Signals - Fluorescence, Phosphorescence, Impedance, Extinction -- Chapter 4. Fluidics -- Chapter 5. Light Sources -- Chapter 6. Optical Benches -- Chapter 7. Detectors and Electronics -- Chapter 8. Signal Analysis -- Chapter 9. The Cytometric File -- Chapter 10. Data Transformation -- Chapter 11. Data Representation -- Chapter 12. Data Analysis -- Chapter 13. Standards, Setup, Calibration, and Control Techniques -- Chapter 14. Fluorochromes ? Overview -- Chapter 15. Fluorochromes Suitable for Antibody Conjugation -- Chapter 16. Fluorochromes That Bind Nucleic Acids -- Chapter 17. Fluorochromes for the Study of the Cell Features -- Chapter 18. Fluorescent Proteins -- Chapter 19. Spillover and Compensation -- Chapter 20. Artifacts -- Chapter 21. Cell Sorting -- Chapter 22. Non-Conventional Flow Cytometry -- Chapter 23. Statistics: A Cytometric Point of View. 330 $aThis book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications. Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process. This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers. 410 0$aBiomedical and Life Sciences Series 606 $aCytology 606 $aBiology$xTechnique 606 $aMedicine$xResearch 606 $aBiology$xResearch 606 $aCell Biology 606 $aBiological Techniques 606 $aBiomedical Research 615 0$aCytology. 615 0$aBiology$xTechnique. 615 0$aMedicine$xResearch. 615 0$aBiology$xResearch. 615 14$aCell Biology. 615 24$aBiological Techniques. 615 24$aBiomedical Research. 676 $a910.5 700 $aOrtolani$b C$g(Claudio),$0292311 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 912 $a9910631093503321 996 $aFlow Cytometry Today$92965719 997 $aUNINA