LEADER 04575nam 22005655 450 001 9910569199503321 005 20180322134112.0 010 $a1-280-35898-X 010 $a9786610358984 010 $a1-59259-936-2 024 7 $a10.1385/1592599362 035 $a(CKB)1000000000239997 035 $a(EBL)3037233 035 $a(SSID)ssj0000194953 035 $a(PQKBManifestationID)11168256 035 $a(PQKBTitleCode)TC0000194953 035 $a(PQKBWorkID)10241126 035 $a(PQKB)11302591 035 $a(DE-He213)978-1-59259-936-3 035 $a(MiAaPQ)EBC3037233 035 $a(PPN)12307892X 035 $a(EXLCZ)991000000000239997 100 $a20100301d2005 u| 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aLymphoma$b[electronic resource] /$fedited by Tim Illidge, Peter W. M. Johnson 205 $a1st ed. 2005. 210 1$aTotowa, NJ :$cHumana Press :$cImprint: Humana,$d2005. 215 $a1 online resource (360 p.) 225 1 $aMethods in Molecular Medicine,$x1543-1894 ;$v115 300 $aDescription based upon print version of record. 311 $a1-58829-159-6 320 $aIncludes bibliographical references and index. 327 $aPurification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins -- Molecular Diagnosis of Lymphoma -- Demonstration of a Germinal Center Immunophenotype in Lymphomas by Immunocytochemistry and Flow Cytometry -- Karyotyping Lymph Node Biopsies in Non-Hodgkin?s Lymphoma -- Identification of Lymphoma-Associated Antigens Using SEREX -- Determining Mutational Status of Immunoglobulin V Genes in Chronic Lymphocytic Leukemia -- Idiotype Gene Rescue in Follicular Lymphoma -- Immunoglobulin Gene Mutation Patterns and Heterogeneity of Marginal Zone Lymphoma -- T-Cell Receptor Molecular Diagnosis of T-Cell Lymphoma -- Cloning of Immunoglobulin Chromosomal Translocations by Long-Distance Inverse Polymerase Chain Reaction -- Sequential Fluorescence In Situ Hybridization Analysis for Trisomy 12 in B-Cell Chronic Lymphocytic Leukemia -- Splenic Marginal Zone Lymphoma -- BCL-6 -- Antibody Techniques Used in the Study of Anaplastic Lymphoma Kinase-Positive ALCL -- Identification of Anaplastic Lymphoma Kinase Variant Translocations Using 5?RACE -- Follicular Lymphoma. 330 $aThe tremendous progress made over the last few years in our molecular understanding of lymphocyte biology and lymphoma classification promises to have a major impact on clinical practice. In Lymphoma: Methods and Protocols, leading clinical investigators from world renowned laboratories describe in step-by-step detail the latest molecular techniques being used to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question. The protocols follow the successful Methods in Molecular Medicine? series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Lymphoma: Methods and Protocols offers oncologists, hematologists, and clinical scientists a diverse collection of readily reproducible techniques for the molecular diagnosis and prognostic evaluation of lymphomas. 410 0$aMethods in Molecular Medicine,$x1543-1894 ;$v115 606 $aOncology 606 $aCancer Research$3http://scigraph.springernature.com/things/product-market-codes/B11001 615 0$aOncology. 615 14$aCancer Research. 676 $a616.99/446 702 $aIllidge$b Tim$4edt$4http://id.loc.gov/vocabulary/relators/edt 702 $aJohnson$b Peter W. M$4edt$4http://id.loc.gov/vocabulary/relators/edt 906 $aBOOK 912 $a9910569199503321 996 $aLymphoma$9874232 997 $aUNINA