LEADER 01302nam--2200397---450- 001 990002084120203316 005 20151009134004.0 035 $a000208412 035 $aUSA01000208412 035 $a(ALEPH)000208412USA01 035 $a000208412 100 $a20041014d1973----km-y0itay0103----ba 101 0 $aita 102 $aIT 105 $a||||||||001yy 200 1 $aEducazione e Politica nel Mezzogiorno d'Italia$e1767-1860$fdi Angelo Broccoli 210 $aFirenze$cLa Nuova Italia$d1973 215 $a250 p.$d25 cm 225 2 $aStorici antichi e moderni$v18 410 0$12001$aStorici antichi e moderni$v18 606 0 $aIstruzione$yItalia meridionale$zSec. 18.-19. 676 $a370.9457 700 1$aBROCCOLI,$bAngelo$0160738 801 0$aIT$bsalbc$gISBD 912 $a990002084120203316 951 $aXXX.A. Coll. 56/ 7 (Coll. DQ 18)$b37311 G.$cXXX.A. Coll. 56/ 7 (Coll. DQ)$d00253227 951 $aXV.9.M. 1489$b3149 MAR$cXV.9.M.$d00347214 959 $aBK 969 $aGIU 969 $aMAR 979 $aSIAV2$b10$c20041014$lUSA01$h1452 979 $aSIAV2$b10$c20041014$lUSA01$h1500 979 $aRSIAV4$b90$c20091210$lUSA01$h1407 979 $aIANNONE$b90$c20151009$lUSA01$h1340 996 $aEducazione e politica nel Mezzogiorno d'Italia$9551419 997 $aUNISA LEADER 04301nam 22004335 450 001 9910563091503321 005 20190412143002.0 010 $a1-4939-7021-6 024 7 $a10.1007/978-1-4939-7021-6 035 $a(CKB)4340000000062474 035 $a(DE-He213)978-1-4939-7021-6 035 $a(OCoLC)1028534965 035 $a(PPN)202988481 035 $a(EXLCZ)994340000000062474 100 $a20170620d2017 u| 0 101 0 $aeng 135 $aurnn#008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$a3D Cell Culture$b[electronic resource] $eMethods and Protocols /$fedited by Zuzana Koledova 205 $a1st ed. 2017. 210 1$aNew York, NY :$cSpringer New York :$cImprint: Humana,$d2017. 215 $a1 online resource (XVI, 452 p. 114 illus., 99 illus. in color.) 225 1 $aMethods in Molecular Biology,$x1064-3745 ;$v1612 311 $a1-4939-7019-4 327 $a3D Cell Culture: An Introduction -- Preparation of Decellularized Biological Scaffolds for 3D Cell Culture -- 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix -- Hydrogel-Based In Vitro Models of Tumor Angiogenesis -- Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels -- Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions.oids -- 3d="" fluid-dynamic="" models="" multiple="" myeloma -- preparation="" three-dimensional="" full="" thickness="" skin="" equivalent -- analysis="" breast="" cell="" invasion="" using="" an="" system -- 3d="" the="" brain="" parenchyma="" metastasis="" interface="" metastasis -- 3d="" neural="" in="" dual="" hydrogel="" systems -- 3d="" micro-patterned="" hydrogels="" prepared="" by="" photomask,="" micro-needle,="" or="" soft="" lithography="" techniques -- 3d="" niche="" engineering="" via="" two-photon="" laser="" polymerization -- microfluidic-based="" generation="" collagen="" spheres="" investigate="" multicellular="" spheroid="" invasion -- high-throughput="" sphere="" formation="" culture -- high-throughput="" tumor="" recyclable="" microfluidic="" platform -- high-throughput="" platform="" mesenchymal="" anastomosed="" microvascular="" network="" living="" capillary="" networks="" and="" endothelial="" cell-lined="" channels -- human="" small="" airway-on-a-chip="" protocol -- microfluidic="" bioprinting="" heterogeneous="" tissue="" constructs -- bioprinting="" decellularized="" extracellular="" matrix="" bioink -- bioprinting="" cartilage="" peg="" hydrogel -- real-time="" cycle="" imaging="" melanoma -- revealing="" ultrastructure="" morphology="" spheroids="" electron="" microscopy -- quantitative="" phenotypic="" image="" analysis="" cultures. 330 $aThis book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. Chapter 15 of this book is available open access under a CC BY 4.0 license. 410 0$aMethods in Molecular Biology,$x1064-3745 ;$v1612 606 $aCytology 606 $aCell Biology$3http://scigraph.springernature.com/things/product-market-codes/L16008 615 0$aCytology. 615 14$aCell Biology. 676 $a571.6 702 $aKoledova$b Zuzana$4edt$4http://id.loc.gov/vocabulary/relators/edt 906 $aBOOK 912 $a9910563091503321 996 $a3D Cell Culture$92835991 997 $aUNINA