LEADER 04425nam 2201081z- 450 001 9910557502303321 005 20231214133036.0 035 $a(CKB)5400000000044533 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/68280 035 $a(EXLCZ)995400000000044533 100 $a20202105d2021 |y 0 101 0 $aeng 135 $aurmn|---annan 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$a3D Stem Cell Culture 210 $aBasel, Switzerland$cMDPI - Multidisciplinary Digital Publishing Institute$d2021 215 $a1 electronic resource (186 p.) 311 $a3-03943-803-4 311 $a3-03943-804-2 330 $aRecently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications. 606 $aResearch & information: general$2bicssc 606 $aBiology, life sciences$2bicssc 610 $ahematopoiesis 610 $ahematopoietic stem cells 610 $astem cell culture 610 $a2D culture 610 $a3D culture 610 $aembryonic stem cells 610 $athree-dimensional 610 $aself-assembling scaffold 610 $apluripotency 610 $aculture conditions 610 $aexpansion 610 $agrowth 610 $aniche 610 $ahuman cortical progenitors 610 $asilicon pillars 610 $acell growth 610 $ahiPSC-derived neural progenitors 610 $acerebral cortex 610 $acarcinogen 610 $aprotein phosphatase 2A (PP2A) 610 $aintestinal tumor 610 $aintestinal organoid 610 $aLgr5+ crypt stem cell 610 $amouse embryonic stem cell 610 $adifferentiation protocol 610 $aureteric bud progenitor cells 610 $a3D kidney organoids 610 $aintestinal organoids 610 $acanine intestine 610 $adifferentiation 610 $aorganoid culture 610 $ainduced pluripotent stem cells 610 $aneurospheres 610 $aneurite outgrowth 610 $aneurotoxicity 610 $ahBM-MSCs 610 $acytokines 610 $atenogenic markers 610 $acyclic strain 610 $a3D microenvironment 610 $aPLGA carriers 610 $abioreactor 610 $acardiac microtissues 610 $aiPSC-derived cardiomyocytes 610 $acardiac fibroblasts 610 $acardiac fibrosis 610 $acardiac rhythm 610 $aTGF-? signalling 610 $adrug screening 610 $ain vitro model 610 $astem cell 610 $a3D 610 $aculture condition 610 $aregenerative medicine 610 $ascaffold 610 $aorganoid 610 $aadipose tissue-derived mesenchymal stem cells 610 $astromal vascular fraction 610 $aplatelet rich plasma 610 $aplatelet concentrates 610 $aveterinary regenerative medicine 615 7$aResearch & information: general 615 7$aBiology, life sciences 700 $aYlostalo$b Joni H$4edt$01289237 702 $aYlostalo$b Joni H$4oth 906 $aBOOK 912 $a9910557502303321 996 $a3D Stem Cell Culture$93021124 997 $aUNINA