LEADER 04464nam 2201117z- 450 001 9910557141203321 005 20210501 035 $a(CKB)5400000000040647 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/68554 035 $a(oapen)doab68554 035 $a(EXLCZ)995400000000040647 100 $a20202105d2021 |y 0 101 0 $aeng 135 $aurmn|---annan 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aSample Preparation in Metabolomics 210 $aBasel, Switzerland$cMDPI - Multidisciplinary Digital Publishing Institute$d2021 215 $a1 online resource (208 p.) 311 08$a3-03943-813-1 311 08$a3-03943-814-X 330 $aMetabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution ? magic-angle spinning nuclear magnetic resonance (HR-?MAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees. 606 $aMedicine and Nursing$2bicssc 610 $a20% FCS 610 $aAMOPLS 610 $aAsPC-1 610 $abiomarkers 610 $acapillary electrophoresis-mass spectrometry 610 $acapillary electrophoresis-mass spectrometry (CE-MS) 610 $acardiovascular disease 610 $acell line 610 $adatabases 610 $adesign of experiments 610 $adiagnostics 610 $adrug discovery 610 $aelectromembrane extraction 610 $aexosomes 610 $aextracellular vesicles 610 $aextraction 610 $aforestry 610 $agas chromatography 610 $agas chromatography-mass spectrometry (GC-MS) 610 $aGC-MS 610 $aharvesting 610 $ahigh resolution mass spectrometry 610 $ahigh-resolution magic angle spinning 610 $ahuman cell line 610 $ahuman milk 610 $ahuman plasma 610 $ahydrophilic interaction liquid chromatography 610 $aion mobility spectrometry 610 $aIT-139 610 $aKP1339 610 $aLC-MS 610 $aLC-MS/MS 610 $alipidomics 610 $aliquid chromatography 610 $aliquid chromatography-mass spectrometry 610 $aliquid chromatography-mass spectrometry (LC-MS) 610 $amass spectrometry 610 $ametabolic pathways 610 $ametabolic profiling 610 $ametabolite extraction 610 $ametabolites 610 $ametabolome 610 $ametabolomics 610 $ametabonomics 610 $ametadata standardization 610 $ametallodrugs 610 $amethod development 610 $aMiaPaCa-2 610 $amicroscopic samples 610 $amicrovesicles 610 $amulti-segment injection 610 $amulticellular tumor spheroids 610 $aNMR 610 $anormalization 610 $anuclear magnetic resonance (NMR) 610 $anuclear magnetic resonance spectroscopy 610 $aoxaliplatin 610 $aPanc-1 610 $aplant metabolomics 610 $aplant natural products 610 $asample preparation 610 $asampling 610 $atrees 615 7$aMedicine and Nursing 700 $aKuligowski$b Julia$4edt$01302968 702 $aQuintás$b Guillermo$4edt 702 $aKuligowski$b Julia$4oth 702 $aQuintás$b Guillermo$4oth 906 $aBOOK 912 $a9910557141203321 996 $aSample Preparation in Metabolomics$93026786 997 $aUNINA LEADER 04273nam 2200985z- 450 001 9910557463103321 005 20210501 035 $a(CKB)5400000000043168 035 $a(oapen)https://directory.doabooks.org/handle/20.500.12854/68337 035 $a(oapen)doab68337 035 $a(EXLCZ)995400000000043168 100 $a20202105d2021 |y 0 101 0 $aeng 135 $aurmn|---annan 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aMathematical Modelling in Biomedicine 210 $aBasel, Switzerland$cMDPI - Multidisciplinary Digital Publishing Institute$d2021 215 $a1 online resource (224 p.) 311 08$a3-03943-493-4 311 08$a3-03943-494-2 330 $aMathematical modelling in biomedicine is a rapidly developing scientific discipline at the intersection of medicine, biology, mathematics, physics, and computer science. Its progress is stimulated by fundamental scientific questions and by the applications to public health. This book represents a collection of papers devoted to mathematical modelling of various physiological problems in normal and pathological conditions. It covers a broad range of topics including cardiovascular system and diseases, heart and brain modelling, tumor growth, viral infections, and immune response. Computational models of blood circulation are used to study the influence of heart arrhythmias on coronary blood flow and on operating modes for left-ventricle-assisted devices. Wave propagation in the cardiac tissue is investigated in order to show the influence of tissue heterogeneity and fibrosis. The models of tumor growth are used to determine optimal protocols of antiangiogenic and radiotherapy. The models of viral hepatitis kinetics are considered for the parameter identification, and the evolution of viral quasi-species is investigated. The book presents the state-of-the-art in mathematical modelling in biomedicine and opens new perspectives in this passionate field of research. 606 $aMathematics & science$2bicssc 606 $aResearch & information: general$2bicssc 610 $a1D haemodynamics 610 $abradycardia 610 $abrain stimulation 610 $acardiac fibrosis 610 $acardiac mechanics 610 $acardiac pacing 610 $acardiomyopathies 610 $acomputer experiment 610 $aconstrained optimization 610 $acoronary circulation 610 $aderivative free optimization 610 $adifferential equations 610 $aelongated obstacle 610 $aexcitable media 610 $agenotype 610 $agradient descent 610 $aheart modeling 610 $aheterogeneity 610 $aimmune response 610 $aintegro-differential equation 610 $ainterventricular asynchrony 610 $aleft ventricle 610 $aleft ventricle remodeling 610 $along QT syndrome 610 $alumped heart model 610 $alymph flow 610 $alymph nodes 610 $alymphatic vessels 610 $amathematical modeling 610 $amathematical modelling 610 $amathematical oncology 610 $amultiscale models 610 $amultiscale simulation 610 $amyocardium 610 $aneural field model 610 $anonlocal interaction 610 $aparameter estimation 610 $apremature ventricular contraction 610 $aquasi-species diversification 610 $areaction-diffusion-convection equations 610 $aresistance to treatment 610 $arotary blood pump 610 $aspatially distributed modeling 610 $aspatially-distributed modeling 610 $aspiral wave 610 $asystole variations 610 $atachycardia 610 $aviral hepatitis 610 $avirus density distribution 610 $avirus infection 610 $awave break 610 $awaves 615 7$aMathematics & science 615 7$aResearch & information: general 700 $aVolpert$b Vitaly$4edt$060607 702 $aVolpert$b Vitaly$4oth 906 $aBOOK 912 $a9910557463103321 996 $aMathematical Modelling in Biomedicine$93026549 997 $aUNINA