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100   $a20190322d2019      uy             0
101 0 $aeng
135   $aurcnu||||||||
181   $ctxt$2rdacontent
182   $cc$2rdamedia
183   $acr$2rdacarrier
200 10$aUnderstanding light microscopy /$fby Jeremy Sanderson
210  1$aHoboken, NJ :$cWiley,$d2019.
215   $a1 online resource (850 pages)
225 1 $aRoyal Microscopical Society/John Wiley Series
311   $a0-470-97375-7 
320   $aIncludes bibliographical references and index.
327   $aOur eyes and the microscope -- Light -- Basic microscope optics -- Microscope design -- Ergonomics -- Optical aberrations of the microscope -- The microscope objective -- Condensers & eyepieces -- Illumination in the microscope -- Diffraction and image formation in microscopy -- Contrast generation & enhancement -- Reflected-light microscopy -- Polarised-light microscopy : part 1, theory -- Polarised-light microscopy : part 2, applied -- Fluorescence microscopy -- Fluorophores & fluorescent proteins -- Optical sectioning & confocal microscopy -- Operating the confocal microscope -- Light-sheet microscopy -- Bleed-through & spectral unmixing -- Deconvolution -- Multi-photon microscopy -- Total internal reflection fluorescence microscopy -- Frap and fret -- Colocalisation -- Super-resolution fluorescence microscopy -- Choosing a microscope platform and core imaging facilities -- Biological specimen preparation -- Materials specimen preparation -- Recording the image : part 1, theory -- Recording the image : part 2, applied.
410  0$aRMS-Wiley series.
606   $aMicroscopy
615  0$aMicroscopy.
676   $a570.28/2
700   $aSanderson$b J. B$g(Jeremy B.),$01218598
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910555273503321
996   $aUnderstanding light microscopy$92818093
997   $aUNINA