LEADER 02825nam 2200529 450 001 9910483440003321 005 20230717143732.0 010 $a3-030-62490-0 024 7 $a10.1007/978-3-030-62490-3 035 $a(CKB)4100000011918836 035 $a(DE-He213)978-3-030-62490-3 035 $a(MiAaPQ)EBC6606062 035 $a(Au-PeEL)EBL6606062 035 $a(OCoLC)1250011614 035 $a(PPN)255884982 035 $a(EXLCZ)994100000011918836 100 $a20220114d2021 uy 0 101 0 $aeng 135 $aurnn#008mamaa 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aNext generation sequencing and data analysis /$fMelanie Kappelmann-Fenzl, editor 205 $a1st ed. 2021. 210 1$aCham, Switzerland :$cSpringer,$d[2021] 210 4$d©2021 215 $a1 online resource (XII, 218 p. 54 illus., 51 illus. in color.) 225 1 $aLearning materials in biosciences 311 $a3-030-62489-7 320 $aIncludes bibliographical references and index. 327 $aChapter 1. Next Generation Sequencing (NGS)- What can be sequenced? -- Opportunities and Perspectives of NGS Applications in Cancer Research -- Chapter 3. Library Construction for NGS -- Chapter 4. NGS Technologies -- Chapter 5. Computer Setup -- Chapter 6. Introduction to Command Line -- Chapter 7. NGS Data -- Chapter 8. Reference Genome -- Chapter 9. Alignment -- Chapter 10. Identification of Genetic Variants and de novo Mutations Based on NGS -- Chapter 11. Design and Analysis of RNA Sequencing Data -- Chapter 12. Design and Analysis of Epigenetics and ChIP-Sequencing Data -- APPENDIX. 330 $aThis textbook provides step-by-step protocols and detailed explanations for RNA Sequencing, ChIP-Sequencing and Epigenetic Sequencing applications. The reader learns how to perform Next Generation Sequencing data analysis, how to interpret and visualize the data, and acquires knowledge on the statistical background of the used software tools. Written for biomedical scientists and medical students, this textbook enables the end user to perform and comprehend various Next Generation Sequencing applications and their analytics without prior understanding in bioinformatics or computer sciences. 410 0$aLearning materials in biosciences. 606 $aSequence alignment (Bioinformatics) 606 $aBiologia computacional$2thub 608 $aLlibres electrònics$2thub 615 0$aSequence alignment (Bioinformatics) 615 7$aBiologia computacional 676 $a572.8 702 $aKappelmann-Fenzl$b Melanie 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910483440003321 996 $aNext Generation Sequencing and Data Analysis$92184191 997 $aUNINA