LEADER 04995nam 22006612 450 001 9910828651903321 005 20151005020623.0 010 $a1-107-23287-2 010 $a1-139-85343-0 010 $a1-139-01817-5 010 $a1-139-84549-7 010 $a1-139-83960-8 010 $a1-139-84198-X 010 $a1-139-84434-2 010 $a1-283-81251-7 010 $a1-139-84079-7 035 $a(CKB)2550000000708673 035 $a(EBL)1057453 035 $a(OCoLC)818658315 035 $a(SSID)ssj0000756859 035 $a(PQKBManifestationID)11390429 035 $a(PQKBTitleCode)TC0000756859 035 $a(PQKBWorkID)10753395 035 $a(PQKB)10793637 035 $a(UkCbUP)CR9781139018173 035 $a(MiAaPQ)EBC1057453 035 $a(Au-PeEL)EBL1057453 035 $a(CaPaEBR)ebr10623117 035 $a(CaONFJC)MIL412501 035 $a(PPN)26133168X 035 $a(EXLCZ)992550000000708673 100 $a20110216d2013|||| uy| 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aScanning electron microscopy for the life sciences /$fHeide Schatten$b[electronic resource] 210 1$aCambridge :$cCambridge University Press,$d2013. 215 $a1 online resource (xi, 261 pages) $cdigital, PDF file(s) 225 1 $aAdvances in microscopy and microanalysis 300 $aTitle from publisher's bibliographic system (viewed on 05 Oct 2015). 311 $a0-521-19599-3 327 $a1. The role of scanning electron microscopy in cell and molecular biology: SEM basics, past accomplishments and new frontiers / Heide Schatten -- 2. Corrosion casting technique / Jerzy Walocha, Jan A. Litwin and Adam J. Miodon?ski -- 3. Revealing the internal structure of cells in three dimensions with scanning electron microscopy / Sol Sepsenwol -- 4. Mitochondria form continuous intracellular network-structures visualized with high-resolution field-emission scanning electron microscopy / T. Naguro, H. Nakane and S. Inaga -- 5. Is the scanning mode the future of electron microscopy in cell biology? / Paul Walther, Christopher Schmid, Michaela Sailer, and Katherine Ho?hn -- 6. High resolution labeling for correlative microscopy / Ralph Albrecht, Daryl A. Meyer and O.E. Olorundare -- 7. The use of SEM to explore virus structure and trafficking / Jens M. Holl and Elizabeth R. Wright -- 8. High resolution scanning electron microscopy of the nuclear surface in Herpes Simplex Virus 1 infected cells / Peter Wild, Andres Kaech and Miriam S. Lucas -- 9. Scanning electron microscopy of chromosomes: structural and analytical investigations / Elizabeth Schroeder-Reiter and Gerhard Wanner -- 10. A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy / Giuseppe Familiari, Rosemarie Heyn, Luciano Petruzziello and Michela Relucenti -- 11. Scanning electron microscopy of cerebellar intrinsic circuits / Orlando J. Castejo?n -- 12. Application of in vivo cryotechnique to living animal organs examined by scanning electron microscopy / Shinichi Ohno, Nobuo Terada, Nobuhiko Ohno and Yasuhisa Fujii -- 13. SEM in dental research / Vladimir Dusevich, Jennifer R. Melander and J. David Eick -- 14. SEM, teeth and palaeoanthropology: the secret of ancient human diets / Alejandro Romero and Joaqui?n De Juan. 330 $aRecent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. 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