LEADER 04293nam 22006615 450 001 9910483125603321 005 20251113210829.0 010 $a981-16-1425-3 024 7 $a10.1007/978-981-16-1425-5 035 $a(CKB)4100000011891294 035 $a(MiAaPQ)EBC6566468 035 $a(Au-PeEL)EBL6566468 035 $a(OCoLC)1247945417 035 $a(PPN)25529106X 035 $a(DE-He213)978-981-16-1425-5 035 $a(EXLCZ)994100000011891294 100 $a20210420d2021 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aAcoustic Levitation-Based Trace-Level Biosensing $eDesign of Detection Systems and Applications to Real Samples /$fby Akihisa Miyagawa 205 $a1st ed. 2021. 210 1$aSingapore :$cSpringer Nature Singapore :$cImprint: Springer,$d2021. 215 $a1 online resource (101 pages) 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5061 311 08$a981-16-1424-5 327 $a1. Introduction -- 2. Theory of Combined Acoustic-Gravitational Field -- 3. Principle of Detection Using Particle Levitation in a Coupled Acoustic-Gravitational Field -- 4. Detection of the Avidin-Biotin Reaction -- 5. Label-Free Detection for the DNA/RNA molecules -- 6. Aptamer-Based Sensing of Small Organic Molecules. 330 $aThis book shows the availability and potential of the coupled acoustic-gravitational (CAG) field for trace-level biosensing. The proposed detection scheme also allows the evaluation of the kinetics and thermodynamics of the reaction occurring on a single microparticle (MP). This method has wide applicability in important fields, involving not only chemistry but also life, environmental, and medical sciences. The author proposes novel trace-level biosensing based on measurements of the levitation coordinate shift of an MP in the CAG field. The levitation coordinate of the MP in the CAG field is determined by its density and compressibility. The levitation coordinate shift is induced by the binding of gold nanoparticles (AuNPs) to the MP through interparticle reactions. Therefore, the quantity of molecules involved in the reaction can be determined from the levitation coordinate shift. The author demonstrates the zmol level detection for biotin, DNA/RNA, and organic molecules. In addition, the kinetics and thermodynamics are evaluated for various reactions occurring between the MP and AuNP, such as the avidin-biotin reaction, direct hybridization, sandwich hybridization, and aptamer-target complexation. This book provides a new concept based on the CAG field, in which the extent of a reaction is converted into the levitation coordinate shift, that is, ?length.? The proposed method has many advantages over other methods, e.g., high biocompatibility, high applicability, and short analysis time. In addition, because the apparatus used in this study is inexpensive and easy to miniaturize, this method is useful in important practical fields, such as forensic and environmental science and diagnosis. Thus, this book inspires many researchers to apply the present method to their own fields of interest. 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5061 606 $aAnalytical chemistry 606 $aChemistry, Physical and theoretical 606 $aMolecular biology 606 $aMeasurement 606 $aMeasuring instruments 606 $aAnalytical Chemistry 606 $aPhysical Chemistry 606 $aMolecular Biology 606 $aMeasurement Science and Instrumentation 615 0$aAnalytical chemistry. 615 0$aChemistry, Physical and theoretical. 615 0$aMolecular biology. 615 0$aMeasurement. 615 0$aMeasuring instruments. 615 14$aAnalytical Chemistry. 615 24$aPhysical Chemistry. 615 24$aMolecular Biology. 615 24$aMeasurement Science and Instrumentation. 676 $a610.28 700 $aMiyagawa$b Akihisa$0849322 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910483125603321 996 $aAcoustic Levitation-Based Trace-Level Biosensing$91896818 997 $aUNINA