LEADER 03882nam 2200697 a 450 001 9910465529303321 005 20200520144314.0 010 $a3-11-048150-2 010 $a3-11-025236-8 024 7 $a10.1515/9783110252361 035 $a(CKB)2560000000079400 035 $a(EBL)835445 035 $a(OCoLC)772845183 035 $a(SSID)ssj0000591898 035 $a(PQKBManifestationID)11391282 035 $a(PQKBTitleCode)TC0000591898 035 $a(PQKBWorkID)10716490 035 $a(PQKB)10072094 035 $a(MiAaPQ)EBC835445 035 $a(DE-B1597)123278 035 $a(OCoLC)979912175 035 $a(OCoLC)984676958 035 $a(OCoLC)987942241 035 $a(OCoLC)992455323 035 $a(OCoLC)999360695 035 $a(DE-B1597)9783110252361 035 $a(Au-PeEL)EBL835445 035 $a(CaPaEBR)ebr10527860 035 $a(CaONFJC)MIL628114 035 $a(EXLCZ)992560000000079400 100 $a20110712d2012 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMethods in protein biochemistry$b[electronic resource] /$fedited by Harald Tschesche 210 $aBerlin $cDe Gruyter$dc2012 215 $a1 online resource (378 p.) 300 $aDescription based upon print version of record. 311 $a1-306-96863-1 311 $a3-11-025233-3 320 $aIncludes bibliographical references and index. 327 $aThree-phase partitioning -- Folding and degradation functions of molecular chaperones -- Membrane protein folding in detergents -- Glycoprotein folding quality control in the endoplasmic reticulum -- Protein import into the intermembrane space of mitochondria -- On-membrane identification of gel-resolved proteins by MALDI-MS -- Analysis of protein complexes using chemical cross-linking and mass spectrometry -- Single-crystal spectroscopy correlated with X-ray crystallography provides complementary perspectives on macromolecular function -- Wide-angle X-ray solution scattering (WAXS) -- Where purity matters : recombinant versus synthetic peptides in beta amyloid formation -- Chemical modification of proteins in living cells -- Proteomics of human bronchoalveolar lavage fluid : discovery of biomarkers of chronic obstructive pulmonary disease with difference gel electrophoresis (DIGE) and mass spectrometry -- Proteomic of Duchenne muscular dystrophy -- Target-oriented peptide arrays in a palliative approach to cystic fibrosis -- Probing protein dynamics in vivo using backbone cyclization : bacterial acyl carrier protein as a case study -- The protein epitope mimetic approach to protein-protein interaction inhibitors -- The structural biology of alpha-1 antitrypsin deficiency and the serpinopathies. 330 $aThis book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in s 606 $aProteins$xAnalysis 606 $aProteins$xSynthesis 606 $aProteomics 608 $aElectronic books. 615 0$aProteins$xAnalysis. 615 0$aProteins$xSynthesis. 615 0$aProteomics. 676 $a572/.67 701 $aTschesche$b Harald$063600 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910465529303321 996 $aMethods in protein biochemistry$92448695 997 $aUNINA