LEADER 05133nam 2200637Ia 450 001 9910462073803321 005 20200520144314.0 010 $a1-283-57266-4 010 $a9786613885111 010 $a0-12-394833-9 035 $a(CKB)2670000000233489 035 $a(EBL)1001358 035 $a(OCoLC)810414726 035 $a(SSID)ssj0000737444 035 $a(PQKBManifestationID)11378476 035 $a(PQKBTitleCode)TC0000737444 035 $a(PQKBWorkID)10786598 035 $a(PQKB)10252491 035 $a(MiAaPQ)EBC1001358 035 $a(Au-PeEL)EBL1001358 035 $a(CaPaEBR)ebr10593898 035 $a(CaONFJC)MIL388511 035 $a(EXLCZ)992670000000233489 100 $a20111102h20122013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aForensic DNA biology$b[electronic resource] $ea laboratory manual /$fKelly M. Elkins 210 $aOxford $cElsevier$d2012, c2013 215 $a1 online resource (225 p.) 300 $aIncludes index. 311 $a0-12-394585-2 327 $aFront Cover; Forensic DNA Biology: A Laboratory Manual; Copyright; Contents; Acknowledgements; About the Author; Welcome; Forensic DNA Biology: An Introduction; BIOLOGY OVERVIEW; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; POLYMERASE CHAIN REACTION; SHORT TANDEM REPEATS; SINGLE NUCLEOTIDE POLYMORPHISMS; MITOCHONDRIAL DNA; KNOWN VERSUS QUESTIONED SAMPLES; WHY STUDY FORENSIC DNA BIOLOGY?; Laboratory Safety; RULES FOR A SAFE LAB ENVIRONMENT; Reference; Avoiding Contamination Issues: Standard Laboratory Practices; Reference; Chapter 1 - Pipetting; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND 327 $aPROCEDUREQUESTIONS; GRAPHING THE DATA USING MICROSOFT EXCEL (2003); EQUATIONS; References; Chapter 2 - Serology; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 3 - Sampling Biological Evidence for DNA Extraction; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE: DNA COLLECTION AND PACKAGING; QUESTIONS; References; Chapter 4 - DNA Extraction; OBJECTIVE; SAFETY; MATERIALS; RECIPES FOR BUFFER AND SOLUTION PREPARATION; BACKGROUND; PROCEDURE; QUESTION; References; Chapter 5 - Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis 327 $aOBJECTIVESAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; Reference; Chapter 6 - Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 7 - Determination of DNA Quantity by Fluorescence Spectroscopy; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 8 - Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References 327 $aChapter 9 - Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico)OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 10 - Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 11 - Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References 327 $aChapter 12 - Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex KitOBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTION; References; Chapter 13 - Computing Random Match Probability from DNA Profile Data Using Population Databases; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 14 - Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References 327 $aChapter 15 - Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit 330 $a DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. 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